Results 41 to 50 of about 24,574 (211)

Genetic characterization of fluoroquinolone-resistant Escherichia coli associated with bovine mastitis in India [PDF]

Veterinary World, 2016
Aim: The present study was undertaken to characterize the mutation in gyrA (DNA gyrase) and parC (topoisomerase IV) genes responsible for fluoroquinolone resistance in Escherichia coli isolates associated with the bovine mastitis. Materials and Methods:
Sangeetha Balakrishnan, Prabhakar Xavier Antony, Hirak Kumar Mukhopadhyay, Raghavan Madhusoodanan Pillai, Jacob Thanislass, Vijayalakshmi Padmanaban, Mouttou Vivek Srinivas   +6 more
doaj   +1 more source

A novel parC mutation potentiating fluoroquinolone resistance in Klebsiella pneumoniae and Escherichia coli clinical isolates

Journal of Infection in Developing Countries, 2022
Introduction: Resistance to fluoroquinolones is mainly due to point mutations that gave rise to amino acid substitutions in the quinolone resistance-determining regions of either gyrA or parC genes, which may be augmented by plasmid mediated resistance.
Marwa Atef Yakout, Ghada Hani Ali
doaj   +1 more source

The role of gyrA, gyrB, and dnaA functions in bacterial conjugation

Annals of Microbiology, 2015
The role of DNA gyrase in F’lac plasmid conjugation was studied using Escherichia coli gyrA43 (Ts), gyrB41(Ts), and dnaA46(Ts) thermosensitive mutants as donor or recipient organisms, and a rifampicin or nalidixic acid-resistant J-53 strain in the presence or absence of nalidixic acid. Mating experiments were also performed employing Hfr derivatives of
Anna Marchese, Eugenio A. Debbia
openaire   +2 more sources

Whole genome sequencing of Mycoplasma hominis strains resistant to ciprofloxacin [PDF]

Клиническая микробиология и антимикробная химиотерапия, 2018
In order to determine molecular mechanisms of resistance to ciprofloxacin, clinical isolates of Mycoplasma hominis (2 strains) obtained from women with pelvic inflammatory disease were investigated.
Kolesnikova E.A., Brusnigina N.F., Makhova M.A., Alekseeva A.E.   +3 more
doaj  

Association of mutation patterns in gyrA/B genes and ofloxacin resistance levels in Mycobacterium tuberculosis isolates from East China in 2009

BMC Infectious Diseases, 2011
Background This study aimed to analyze the association of mutation patterns in gyrA and gyrB genes and the ofloxacin resistance levels in clinical Mycobacterium tuberculosis isolates sampled in 2009 from East China.
Huang Xiaochen, Lu Junmei, Wang Jie, Cui Zhenling, Hu Zhongyi   +4 more
doaj   +1 more source

MYCOBACTERIUM TUBERCULOSIS AND gyrA VARIATION IN ZAMBIA

Tropical Medicine and Health, 2005
M. tuberculosis strains were isolated from clinically and bacteriologically confirmed patients to evaluate the susceptibility of clinical M. tuberculosis isolates to fluoroquinolone and to obtain molecular epidemiological information in Zambia,.
MITARAI, SATOSHI, KAFWABULULA, LYNDON M, HABEENZU, CHARITY, TERUNUMA, HIROSHI, LUBASI, DAVID, KASOLO, FRANCIS C, SHISHIDO, HARUMI, NUMAZAKI, YOSHIO   +7 more
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Moxifloxacin Retains Antimycobacterial Activity in the Presence of gyrA Mutations [PDF]

Antimicrobial Agents and Chemotherapy, 2014
ABSTRACT Moxifloxacin-resistant Mycobacterium tuberculosis mutants were selected in vitro using different concentrations of moxifloxacin. gyrA mutations at codons 88 and 94 were associated with resistance (defined as an MIC of ≥2 μg/ml) ( P
Marieta, McGrath, Nico C, Gey van Pittius, Frederick A, Sirgel, Paul D, Van Helden, Robin M, Warren   +4 more
openaire   +2 more sources

Molecular characteristics of fluoroquinolone-resistant Mycobacterium tuberculosis strains from newly diagnosed tuberculosis patients in the Northwest of Russia

Журнал микробиологии, эпидемиологии и иммунобиологии
Introduction. Fluoroquinolones remain the key second-line anti-tuberculosis drugs. The aim of the study was the molecular characterization of fluoroquinolone-resistant Mycobacterium tuberculosis strains from newly diagnosed tuberculosis patients in ...
Anna A. Vyazovaya, Natalia S. Solovieva, Alena A. Gerasimova, Viacheslav Yu. Zhuravlev, Igor V. Mokrousov   +4 more
doaj   +1 more source

An Evolved Mxe GyrA Intein for Enhanced Production of Fusion Proteins [PDF]

ACS Chemical Biology, 2014
Expressing antibodies as fusions to the non-self-cleaving Mxe GyrA intein enables site-specific, carboxy-terminal chemical modification of the antibodies by expressed protein ligation (EPL). Bacterial antibody-intein fusion protein expression platforms typically yield insoluble inclusion bodies that require refolding to obtain active antibody-intein ...
Marshall, Carrie J., Grosskopf, Vanessa A., Moehling, Taylor J., Tillotson, Benjamin J., Wiepz, Gregory J., Abbott, Nicholas L., Raines, Ronald T., Shusta, Eric V.   +7 more
openaire   +2 more sources

gyrA and gyrB mutations in quinolone-resistant strains of Escherichia coli [PDF]

Antimicrobial Agents and Chemotherapy, 1989
The proportion of gyrA and gyrB mutations in quinolone-resistant Escherichia coli strains was examined by introducing cloned wild-type gyrA and gyrB genes. In 25 spontaneous mutants of strain KL16, 13 had gyrA and 12 had gyrB mutations. In eight clinical isolates, five had gyrA mutations and one had a gyrB mutation; mutations in two isolates remained ...
S, Nakamura, M, Nakamura, T, Kojima, H, Yoshida   +3 more
openaire   +2 more sources