Elucidation of the disulfide folding pathway of hirudin by a topology-based approach [PDF]
, 2003 A theoretical model for the folding of proteins containing disulfide bonds is introduced. The model exploits the knowledge of the native state to favour the progressive establishment of native interactions.
De Filippis, V. +3 more
core +2 more sources
Lipolysis generates platelet dysfunction after in vivo heparin administration [PDF]
, 2002 Heparin, when administered to patients undergoing operations using cardiopulmonary bypass, induces plasma changes that gradually impair platelet macroaggregation, but heparinization of whole blood in vitro does not have this effect.
Belcher, P.R. +5 more
core +1 more source
Folding of small disulfide-rich proteins : clarifying the puzzle [PDF]
, 2006 Premi a l'excel·lència investigadora. Àmbit de les Ciències Experimentals. 2008The process by which small proteins fold to their native conformations has been intensively studied over the last few decades.
Apuy +73 more
core +2 more sources
Cell Transplantation, 2006 Human islets induce an injurious clotting reaction at the time of transplantation. A potential strategy to counteract this reaction would be to allow the islets to express hirudin, a protein with direct anticoagulative activity.
Sanja Cabric +4 more
doaj +1 more source
Effect of the administration of recombinant hirudin and/or tissue-plasminogen activator (t-PA) on endotoxin-induced disseminated intravascular coagulation model in rabbits [PDF]
, 1999 We evaluated the effect of r-hirudin and/or tissue-plasminogen activator (t-PA) in a model of DIC in rabbits induced by i.v. infusion of 100 micrograms/kg/h/6 h endotoxin. Rabbits were treated with saline (endotoxin control group), r-hirudin at 0.3 mg/kg/
Hermida, J. (José) +5 more
core +1 more source
Pharmacokinetics study of recombinant hirudin in the plasma of rats using chromogenic substrate, ELISA, and radioisotope assays. [PDF]
PLoS ONE, 2013 AIM: To compare the analytical methods used to study the pharmacokinetics of recombinant hirudin in the plasma of rats that had been injected with (125)I-recombinant hirudin.
Su-Yun Jiang +3 more
doaj +1 more source
Laryngeal Leech as a Live Foreign Body: Case Reports and a Safe Removal Technique [PDF]
Case Reports in OtolaryngologyLeech infestation in the larynx is a rare condition with serious implications due to airway obstruction and persistent bleeding. This report presents two cases of dysphonia and hemoptysis caused by laryngeal leeches after consuming contaminated water.
Mohebbi M +6 more
europepmc +2 more sources
Non-canonical proteolytic activation of human prothrombin by subtilisin from Bacillus subtilis may shift the procoagulant\ue2\u80\u93anticoagulant equilibrium toward thrombosis [PDF]
, 2017 Blood coagulation is a finely regulated physiological process culminating with the factor Xa (FXa)-mediated conversion of the prothrombin (ProT) zymogen to active -thrombin (T).
Acquasaliente, Laura +5 more
core +1 more source
Microchip electrophoresis bioanalytical applications [PDF]
, 2008 Microchip electrophoresis (MCE) is a novel analytical technique resulting from miniaturization of capillary electrophoresis (CE) to a planar microfabricated separation device.
Vlcková, Markéta
core +1 more source
The effect of recombinant hirudin on rabbit ear flaps with venous insufficiency
Indian Journal of Plastic Surgery, 2014 The effect of recombinant hirudin, which is the most powerful antithrombotic agent, on flaps with venous insufficiency was investigated. Oedema and congestion are frequent on flaps, causing necrosis unpredictably.
Serdar Duzgun +2 more
doaj +1 more source