Results 1 to 10 of about 2,427 (116)

Directed evolution of the pathogenic mold Aspergillus fumigatus reveals novel genes contributing to triazole resistance [PDF]

Antimicrobial Agents and Chemotherapy
Aspergillus fumigatus is the leading cause of invasive mold infections in immunocompromised patients. Current antifungal treatment primarily depends on the triazole antifungals, which act by inhibiting Erg11/Cyp51, a key enzyme in the ergosterol ...
Mariana Handelman, Argha Sarkar, Avital Varshavsky, Yona Shadkchan, Xuefei Chen, Gerard D. Wright, Endrews Delbaje, Bradley Laflamme, Sara Fallah, Evelyne Côté, Nicole Robbins, Thaila Fernanda dos Reis, Gustavo H. Goldman, Leah E. Cowen, Nir Osherov   +14 more
doaj   +2 more sources

Understanding multi-azole resistance in Aspergillus fumigatus: the impact of non-cyp51A mutation and efflux pump overexpression [PDF]

Frontiers in Fungal Biology
IntroductionAzole resistance in Aspergillus fumigatus is an emerging global concern, significantly limiting treatment options for aspergillosis. The present study aimed to investigate azole resistance mechanisms, focusing on non-cyp51A mutations and ...
Pooja Sen, Lokesh Kumar, Jata Shankar, Pooja Vijayaraghavan   +3 more
doaj   +2 more sources

Environmental Circulation of Aspergillus fumigatus With Reduced Susceptibility to Agricultural Triazole in Brazil: Clonal Dissemination of Potentially Resistant Genotypes. [PDF]

Mycoses
ABSTRACT Background Aspergillus fumigatus resistance to triazole antifungals poses an increasing global health concern. Moreover, the cross‐resistance between azole antifungal agents used in clinical settings and those applied in agriculture has become an important emerging issue.
de Barros Rodrigues DK, Leeuwerik M, Brankovics B, Fava WS, Venturini J, Meyer W, Arai T, Majima H, Watanabe A, de Souza Carvalho Melhem M.   +9 more
europepmc   +2 more sources

Molecular and Phenotypic Characterization of Multidrug-Resistant Aspergillus fumigatus Clinical Isolates in Republic of Korea [PDF]

Journal of Fungi
Genetic diversity and antifungal susceptibility profiles of Aspergillus fumigatus are critical for understanding the evolution of resistance in clinical and environmental settings. We performed comprehensive genomic characterization of A.
Yun Ha Lee, Yewon An, Yu Jin Lee, Jihee Lee, Su Yeon Kim, Byung Hak Kang   +5 more
doaj   +2 more sources

Epidemiology, antifungal susceptibility and biological characteristics of clinical Aspergillus fumigatus in a tertiary hospital [PDF]

Scientific Reports
Azole resistance in Aspergillus fumigatus poses a significant clinical challenge globally. In this study, we analyzed 307 clinical A. fumigatus isolates from General Hospital of Ningxia Medical University in China, collected between July 2023 and July ...
Yuting Kang, Wanting Ma, Qiujie Li, Pengtao Wang, Wei Jia   +4 more
doaj   +2 more sources

Integrative Transcriptomic and Target Metabolite Analysis as a New Tool for Designing Metabolic Engineering in Yeast [PDF]

Biomolecules
Precision fermentation processes, especially when using edited microorganisms, demand accuracy in the bioengineering process to maximize the desired outcome and to avoid adverse effects.
Alejandro Lopez-Barbera, Nerea Abasolo, Helena Torrell, Nuria Canela, Salvador Fernández-Arroyo   +4 more
doaj   +2 more sources

Lanosterol synthase inhibition as a potential approach for re-sensitizing triazole-resistant Aspergillus fumigatus [PDF]

mBio
The increasing prevalence of triazole resistance among Aspergillus fumigatus clinical isolates threatens the efficacy of this front-line treatment, highlighting the urgent need for novel therapeutic targets. In this study, we characterized three putative
Jinhong Xie, Xabier Guruceaga, Adela Martin-Vicente, Harrison I. Thorn, Devi Bale, Uxue Perez Cuesta, Asmita Nandi, Chendo Dieleman, Josie E. Parker, Steven L. Kelly, Christoph Müller, Fabio Gsaller, Jarrod R. Fortwendel   +12 more
doaj   +2 more sources

A secondary mechanism of action for triazole antifungals in Aspergillus fumigatus mediated by hmg1 [PDF]

Nature Communications
Triazole antifungals function as ergosterol biosynthesis inhibitors and are frontline therapy for invasive fungal infections, such as invasive aspergillosis.
Jeffrey M. Rybak, Jinhong Xie, Adela Martin-Vicente, Xabier Guruceaga, Harrison I. Thorn, Ashley V. Nywening, Wenbo Ge, Ana C. O. Souza, Amol C. Shetty, Carrie McCracken, Vincent M. Bruno, Josie E. Parker, Steven L. Kelly, Hannah M. Snell, Christina A. Cuomo, P. David Rogers, Jarrod R. Fortwendel   +16 more
doaj   +2 more sources

High prevalence of azole resistance among environmental Aspergillus fumigatus isolates from outdoor air in Madrid, Spain [PDF]

Frontiers in Microbiology
IntroductionAspergillus fumigatus has been designated by the World Health Organization (WHO) as a critical fungal pathogen. Its spores are commonly present in the air and are inhaled daily.
Juan Carlos Soto-Debrán, Francisco Javier Sánchez-Íñigo, Alejandro B. Calvo-López, Laura Alguacil-Cuéllar, Anastasiia A. Hrynzovska, Anastasiia A. Hrynzovska, Emilia Mellado, Emilia Mellado, Saul García Dos Santos, Laura Alcazar-Fuoli, Laura Alcazar-Fuoli, Ana Alastruey-Izquierdo, Ana Alastruey-Izquierdo   +12 more
doaj   +2 more sources

Genome-wide CRISPRi screen and proteomic profiling identify key genes related to ferulic acid’s antifungal activity [PDF]

mBio
Fungal pathogens of plants must overcome host-imposed stressors, including antimicrobial small molecules. Ferulic acid (FA), a plant-derived phenolic compound, induces fungal stress and cell death.
Ofri Levi, Rina Zuchman, Nour Sleman, Roni Koren, Hazem Khamaisi, Benjamin A. Horwitz   +5 more
doaj   +2 more sources