Results 11 to 20 of about 2,854 (146)

Hmg1 Gene Mutation Prevalence in Triazole-Resistant Aspergillus fumigatus Clinical Isolates

Journal of Fungi, 2020
Recently, mutations in the 3-hydroxy-3-methylglutaryl-coenzyme-A-reductase-encoding gene (hmg1), a gene involved in ergosterol production, were associated with triazole-resistance in Aspergillus fumigatus.
Agustin Resendiz-Sharpe, Margriet W.J. Hokken, Toine Mercier, Rita Merckx, Kamiel Verhagen, Lisa Dewitte, Willem J.G. Melchers, Paul E. Verweij, Johan Maertens, Katrien Lagrou   +9 more
doaj   +2 more sources

Recombinant HMG1 Protein Produced in Pichia pastoris: A Nonviral Gene Delivery Agent

BioTechniques, 1997
This paper describes the production of a recombinant protein from the expression system based on the methylotrophic yeast Pichia pastoris. Efficient production of rat high-mobility-group 1 (HMG1) protein was obtained using the system.
A.R. Mistry, L. Falciola, L. Monaco, R. Tagliabue, G. Acerbis, A. Knight, R.P. Harbottle, M. Soria, M.E. Bianchi, C. Coutelle, S.L. Hart   +10 more
doaj   +2 more sources

Mutations in hmg1, Challenging the Paradigm of Clinical Triazole Resistance in Aspergillus fumigatus [PDF]

mBio, 2019
Aspergillus fumigatus is the predominant pathogen of invasive aspergillosis, a disease state credited with over 200,000 life-threatening infections each year.
Jeffrey M. Rybak, Wenbo Ge, Nathan P. Wiederhold, Josie E. Parker, Steven L. Kelly, P. David Rogers, Jarrod R. Fortwendel   +6 more
doaj   +2 more sources

Unveiling the genetic basis of floral scent formation in roses using weighted gene co-expression and protein-protein interaction network analyses [PDF]

Scientific Reports
Rosa species hold considerable economic and medicinal importance, used in traditional medicine, essential oils, and landscaping. However, the mechanisms of floral scent formation in roses are not well understood, hindering genetic improvement.
Chan Xu, Yuan Chen, Qiaoli Xie, Zongli Hu, Hang Guo, Guoping Chen, Shibing Tian   +6 more
doaj   +2 more sources

hapE and hmg1 Mutations Are Drivers of cyp51A-Independent Pan-Triazole Resistance in an Aspergillus fumigatus Clinical Isolate

Microbiology Spectrum, 2023
Aspergillus fumigatus is a ubiquitous environmental mold that can cause severe disease in immunocompromised patients and chronic disease in individuals with underlying lung conditions. Triazoles are the most widely used class of antifungal drugs to treat
Ana C. O. Souza, Wenbo Ge, Nathan P. Wiederhold, Jeffrey M. Rybak, Jarrod R. Fortwendel, P. David Rogers   +5 more
doaj   +1 more source

Identification and Analysis of Reference and Tissue-Specific Genes in Bitter Gourd Based on Transcriptome Data

Horticulturae, 2023
Accurate and standardized quantification of reverse transcription PCR (qRT-PCR) results relies on the use of a dependable reference gene. The precise control of transgene expression in terms of both spatial and temporal aspects necessitates the ...
Yangyi Zheng, Yao Ma, Jianning Luo, Junxing Li, Xiaoming Zheng, Hao Gong, Liting Deng, Gangjun Zhao, Caixia Luo, Xiaoxi Liu, Haibin Wu   +10 more
doaj   +1 more source

Efficient Generation of Multiple Seamless Point Mutations Conferring Triazole Resistance in Aspergillus fumigatus

Journal of Fungi, 2023
Aspergillus fumigatus is a common human fungal pathogen that can cause a range of diseases. Triazoles are used to treat A. fumigatus infections, but resistance is increasing due to mutations in genes such as cyp51A, hmg1 and overexpression of efflux ...
Mariana Handelman, Nir Osherov
doaj   +1 more source

A Whole Genome Sequencing-Based Approach to Track down Genomic Variants in Itraconazole-Resistant Species of Aspergillus from Iran

Journal of Fungi, 2022
The antifungal resistance in non-fumigatus Aspergillus spp., as well as Aspergillus fumigatus, poses a major therapeutic challenge which affects the entire healthcare community.
Sanaz Nargesi, Reza Valadan, Mahdi Abastabar, Saeed Kaboli, Jose Thekkiniath, Mohammad Taghi Hedayati   +5 more
doaj   +1 more source

A genetic and pharmacological analysis of isoprenoid pathway by LC-MS/MS in fission yeast. [PDF]

PLoS ONE, 2012
Currently, statins are the only drugs acting on the mammalian isoprenoid pathway. The mammalian genes in this pathway are not easily amenable to genetic manipulation.
Tomonori Takami, Yue Fang, Xin Zhou, Wurentuya Jaiseng, Yan Ma, Takayoshi Kuno   +5 more
doaj   +1 more source

Are Point Mutations in HMG-CoA Reductases (Hmg1 and Hmg2) a Step towards Azole Resistance in Aspergillus fumigatus?

Molecules, 2021
Invasive aspergillosis, mainly caused by Aspergillus fumigatus, can lead to severe clinical outcomes in immunocompromised individuals. Antifungal treatment, based on the use of azoles, is crucial to increase survival rates.
Irene Gonzalez-Jimenez, Jose Lucio, Alejandra Roldan, Laura Alcazar-Fuoli, Emilia Mellado   +4 more
doaj   +1 more source