Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform. [PDF]
PLoS ONE, 2017 Illumina's MiSeq has become the dominant platform for gene amplicon sequencing in microbial ecology studies; however, various technical concerns, such as reproducibility, still exist.
Chongqing Wen +10 more
doaj +6 more sources
Comparison of SARS-CoV-2 sequencing using the ONT GridION and the Illumina MiSeq [PDF]
BMC Genomics, 2022 Background Over 4 million SARS-CoV-2 genomes have been sequenced globally in the past 2 years. This has been crucial in elucidating transmission chains within communities, the development of new diagnostic methods, vaccines, and antivirals.
Derek Tshiabuila +12 more
doaj +3 more sources
Characterization of Microsatellite Markers for Pinedrops, Pterospora andromedea (Ericaceae), from Illumina MiSeq sequencing [PDF]
Applications in Plant Sciences, 2014 Premise of the study: Pterospora andromedea (Ericaceae) is a mycoheterotrophic plant endemic to North America with a disjunct distribution. Eastern populations are in decline compared to western populations.
Lisa C. Grubisha +4 more
doaj +3 more sources
A tale of three next generation sequencing platforms: comparison of Ion Torrent, Pacific Biosciences and Illumina MiSeq sequencers [PDF]
BMC Genomics, 2012 Background Next generation sequencing (NGS) technology has revolutionized genomic and genetic research. The pace of change in this area is rapid with three major new sequencing platforms having been released in 2011: Ion Torrent’s PGM, Pacific ...
Quail Michael A +8 more
doaj +4 more sources
Illumina MiSeq sequencing disfavours a sequence motif in the GFP reporter gene. [PDF]
Sci Rep, 2016 AbstractGreen fluorescent protein (GFP) is one of the most used reporter genes. We have used next-generation sequencing (NGS) to analyse the genetic diversity of a recombinant influenza A virus that expresses GFP and found a remarkable coverage dip in the GFP coding sequence.
Van den Hoecke S, Verhelst J, Saelens X.
europepmc +5 more sources
MethodsX, 2023 During the last years, the application of next-generation sequencing (NGS) technologies to search for specific genetic markers has become a crucial method for the characterization of microbial communities.
Alexandra Coello-Camba +2 more
doaj +1 more source
Analysis, optimization and verification of Illumina-generated 16S rRNA gene amplicon surveys. [PDF]
PLoS ONE, 2014 The exploration of microbial communities by sequencing 16S rRNA genes has expanded with low-cost, high-throughput sequencing instruments. Illumina-based 16S rRNA gene sequencing has recently gained popularity over 454 pyrosequencing due to its lower ...
Michael C Nelson +4 more
doaj +1 more source
Development of microsatellite markers using Illumina MiSeq sequencing to characterize Ephedra gerardiana (Ephedraceae). [PDF]
Appl Plant Sci, 2017 Premise of the study:Ephedra gerardiana (Ephedraceae), occurring in the Himalayan ranges, is an important plant species used in Tibetan medicine. Due to the lack of molecular markers to characterize genetic diversity, knowledge for conservation and uses of E. gerardiana resources is limited; we therefore developed microsatellite markers for use in this
De J, Zhu W, Liu T, Wang Z, Zhong Y.
europepmc +3 more sources
Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]
, 2020 BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer +3 more
core +1 more source
Choosing a benchtop sequencing machine to characterise Helicobacter pylori genomes. [PDF]
PLoS ONE, 2013 The fully annotated genome sequence of the European strain, 26695 was first published in 1997 and, in 1999, it was directly compared to the USA isolate J99, promoting two standard laboratory isolates for Helicobacter pylori (H. pylori) research. With the
Timothy T Perkins +3 more
doaj +1 more source