Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices
BMC Genomics, 2018 Background Prosthetic joint infections are clinically difficult to diagnose and treat. Previously, we demonstrated metagenomic sequencing on an Illumina MiSeq replicates the findings of current gold standard microbiological diagnostic techniques ...
Nicholas D Sanderson +11 more
doaj +1 more source
Frontiers in Microbiology, 2020 We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities and companies) from Germany and Austria ...
Laura Uelze +12 more
doaj +1 more source
The unseen world: environmental microbial sequencing and identification methods for ecologists [PDF]
, 2014 Archaea, bacteria, microeukaryotes, and the viruses that infect them (collectively “microorganisms”) are foundational components of all ecosystems, inhabiting almost every imaginable environment and comprising the majority of the planet’s organismal and ...
Aronson, Emma +4 more
core +1 more source
Phylogenomic analysis of lactobacillus curvatus reveals two lineages distinguished by genes for fermenting plant-derived carbohydrates [PDF]
, 2018 Lactobacillus curvatus is a lactic acid bacterium encountered in many different types of fermented food (meat, seafood, vegetables, and cereals). Although this species plays an important role in the preservation of these foods, few attempts have been ...
Chaillou, Stéphane +5 more
core +3 more sources
16S Sequencing on Illumina MiSeq Platform v1
The 16S protocol detailed here is designed to amplify bacteria using V4-V5 hyper-variable regions of the 16S rRNA gene on the Illumina MiSeq DNA sequencer.
Angela Schmoldt, Melissa Schussman
openaire +1 more source
Nanopore duplex sequencing as an alternative to Illumina MiSeq sequencing for eDNA-based biomonitoring of coastal aquaculture impacts [PDF]
Metabarcoding and MetagenomicsOxford Nanopore Technologies has recently launched a duplex sequencing strategy and announced an improved error rate which is in a similar order of magnitude as Illumina sequencing.
Thorsten Stoeck +3 more
doaj +3 more sources
Complete genome sequence of bovine polyomavirus type 1 from aborted cattle, isolated in Belgium in 2014 [PDF]
, 2016 <p>The complete and fully annotated genome sequence of a bovine polyomavirus type 1 (BPyV/BEL/1/2014) from aborted cattle was assembled from a metagenomics data set. The 4,697-bp circular dsDNA genome contains 6 protein-coding genes.
Behaeghel, Isabelle +7 more
core +2 more sources
Rabies Virus Sequencing using Illumina- MiSeq v1
This Rabies whole genome sequencing protocol has been derived and modified from the Illumina COVIDSeq RUO sequencing pipeline. The protocol has been modified and optimised for sequencing Rabies virus (RABV). The methodology uses RABV-specific primers that have been designed in-house.
Chakrakodi N Varun +3 more
openaire +1 more source
BMC Research Notes, 2022 Objectives These data were collected to generate a novel reference metagenome for the sponge Halichondria panicea and its microbiome for subsequent differential expression analyses.
Brian W. Strehlow +3 more
doaj +1 more source
A simple novel device for air sampling by electrokinetic capture. [PDF]
, 2015 BackgroundA variety of different sampling devices are currently available to acquire air samples for the study of the microbiome of the air. All have a degree of technical complexity that limits deployment.
Frazier, Angel +5 more
core +3 more sources