Results 41 to 50 of about 73,480 (161)

RPA is an initiation factor for human chromosomal DNA replication [PDF]

Nucleic Acids Research, 2003
The initiation of chromosomal DNA replication in human cell nuclei is not well understood because of its complexity. To allow investigation of this process on a molecular level, we have recently established a cell-free system that initiates chromosomal DNA replication in an origin-specific manner under cell cycle control in isolated human cell nuclei ...
Dávid, Szüts, Lisa, Kitching, Christo, Christov, Aidan, Budd, Sew, Peak-Chew, Torsten, Krude   +5 more
openaire   +2 more sources

Real-time analysis of initiation regulation systems during the progression of the reconstituted chromosomal replication cycle

Scientific Reports
Chromosome replication in Escherichia coli is primarily regulated at the initiation stage, where the DnaA protein activates replication at the chromosomal origin, oriC.
Koki Kanoh, Masayuki Su’etsugu
doaj   +1 more source

Multiple regulatory mechanisms to inhibit untimely initiation of DNA replication are important for stable genome maintenance. [PDF]

PLoS Genetics, 2011
Genomic instability is a hallmark of human cancer cells. To prevent genomic instability, chromosomal DNA is faithfully duplicated in every cell division cycle, and eukaryotic cells have complex regulatory mechanisms to achieve this goal.
Seiji Tanaka, Hiroyuki Araki
doaj   +1 more source

DnaC inactivation in Escherichia coli K-12 induces the SOS response and expression of nucleotide biosynthesis genes.

PLoS ONE, 2008
BackgroundInitiation of chromosome replication in E. coli requires the DnaA and DnaC proteins and conditionally-lethal dnaA and dnaC mutants are often used to synchronize cell populations.Methodology/principal findingsDNA microarrays were used to measure
Anders Løbner-Olesen, Monika Slominska-Wojewodzka, Flemming G Hansen, Martin G Marinus   +3 more
doaj   +1 more source

Molecular Dissection of the Essential Features of the Origin of Replication of the Second Vibrio cholerae Chromosome

mBio, 2015
Vibrionaceae family members are interesting models for studying DNA replication initiation, as they contain two circular chromosomes. Chromosome II (chrII) replication is governed by two evolutionarily unique yet highly conserved elements, the origin DNA
Matthew A. Gerding, Michael C. Chao, Brigid M. Davis, Matthew K. Waldor   +3 more
doaj   +1 more source

The DnaK Chaperone Uses Different Mechanisms To Promote and Inhibit Replication of Vibrio cholerae Chromosome 2

mBio, 2017
Replication of Vibrio cholerae chromosome 2 (Chr2) depends on molecular chaperone DnaK to facilitate binding of the initiator (RctB) to the replication origin.
Jyoti K. Jha, Mi Li, Rodolfo Ghirlando, Lisa M. Miller Jenkins, Alexander Wlodawer, Dhruba Chattoraj   +5 more
doaj   +1 more source

Chromosome 1 licenses chromosome 2 replication in Vibrio cholerae by doubling the crtS gene dosage.

PLoS Genetics, 2018
Initiation of chromosome replication in bacteria is precisely timed in the cell cycle. Bacteria that harbor multiple chromosomes face the additional challenge of orchestrating replication initiation of different chromosomes.
Revathy Ramachandran, Peter N Ciaccia, Tara A Filsuf, Jyoti K Jha, Dhruba K Chattoraj   +4 more
doaj   +1 more source

End of the beginning: elongation and termination features of alternative modes of chromosomal replication initiation in bacteria. [PDF]

PLoS Genetics, 2015
In bacterial cells, bidirectional replication of the circular chromosome is initiated from a single origin (oriC) and terminates in an antipodal terminus region such that movement of the pair of replication forks is largely codirectional with ...
Jayaraman Gowrishankar
doaj   +1 more source

Unique and universal features of Epsilonproteobacterial origins of chromosome replication and DnaA-DnaA box interactions

Frontiers in Microbiology, 2016
In bacteria, chromosome replication is initiated by the interaction of the initiator protein DnaA with a defined region of a chromosome at which DNA replication starts (oriC).
Pawel Jaworski, Rafal Donczew, Thorsten Mielke, Marcel Thiel, Stanislaw Oldziej, Christoph Weigel, Anna Magdalena Pawlik   +6 more
doaj   +1 more source

A new class of disordered elements controls DNA replication through initiator self-assembly

eLife, 2019
The initiation of DNA replication in metazoans occurs at thousands of chromosomal sites known as origins. At each origin, the Origin Recognition Complex (ORC), Cdc6, and Cdt1 co-assemble to load the Mcm2-7 replicative helicase onto chromatin.
Matthew W Parker, Maren Bell, Mustafa Mir, Jonchee A Kao, Xavier Darzacq, Michael R Botchan, James M Berger   +6 more
doaj   +1 more source