Results 21 to 30 of about 5,753 (209)

Growth behavior and mRNA expression profiling during growth of IPEC-J2 cells. [PDF]

BMC Res Notes
Abstract Objective The IPEC-J2 cell line is used as an in vitro small intestine model for swine, but it is also used as a model for the human intestine, presenting a relatively unique setting. By combining electric cell-substrate impedance sensing, with next-generation-sequencing technology, we showed that mRNA gene ...
Binder ARD, Mussack V, Kirchner B, Pfaffl MW.   +3 more
europepmc   +6 more sources

STIM1 promotes IPEC-J2 porcine epithelial cell restitution by TRPC1 signaling

Animal Biotechnology, 2021
Intestinal epithelial restitution is partly dependent on cell migration, which reseals superficial wounding after injury. Here, we tested the hypothesis that stromal interaction molecule 1(STIM1) regulates porcine intestinal epithelial cell migration by activating transient receptor potential canonical 1 (TRPC1) signaling.
Weiwei Mo, Guangmang Liu, Caimei Wu, Gang Jia, Hua Zhao, Xiaoling Chen, Jing Wang   +6 more
openaire   +2 more sources

Trolox and ascorbic acid reduce direct and indirect oxidative stress in the IPEC-J2 cells, an in vitro model for the porcine gastrointestinal tract [PDF]

, 2015
Oxidative stress in the small intestinal epithelium is a major cause of barrier malfunction and failure to regenerate. This study presents a functional in vitro model using the porcine small intestinal epithelial cell line IPEC-J2 to examine the effects ...
De Smet, Stefaan, Degroote, Jeroen, Jennes, Karen, Michiels, Joris, Tembuyzer, Bart, Van Ginneken, Chris, Vergauwen, Hans, Wang, Wei   +7 more
core   +11 more sources

Lycopene Maintains Mitochondrial Homeostasis to Counteract the Enterotoxicity of Deoxynivalenol

Antioxidants, 2023
The intestinal tract is a target organ for Deoxynivalenol (DON) absorption and toxicity. Mitochondrial homeostasis imbalance is the gut toxicity mechanism of DON. Lycopene (LYC) has intestinal protective effects and can maintain mitochondrial homeostasis
Zihui Cai, Fengjuan Chen, Youshuang Wang, Xuebing Wang, Xu Yang, Cong Zhang   +5 more
doaj   +1 more source

In vitro characterization of TMPRSS2 inhibition in IPEC-J2 cells [PDF]

Journal of Enzyme Inhibition and Medicinal Chemistry, 2016
The transmembrane serine protease, TMPRSS2 is an important target in the treatment of seasonal influenza infections and contributes to prostate carcinogenesis and metastasis. In this study, the effect of the synthetic TMPRSS2 inhibitor I-432 on jejunal IPEC-J2 cell monolayers cultured on membrane inserts was characterized. Using a fluorogenic substrate,
Pászti-Gere, Erzsébet, Czimmermann, Eszter, Ujhelyi, Gabriella, Balla, Péter, Maiwald, Alexander, Steinmetzer, Torsten   +5 more
openaire   +3 more sources

Comparing Two Intestinal Porcine Epithelial Cell Lines (IPECs): Morphological Differentiation, Function and Metabolism. [PDF]

PLoS ONE, 2015
The pig shows genetical and physiological resemblance to human, which predestines it as an experimental animal model especially for mucosal physiology. Therefore, the intestinal epithelial cell lines 1 and J2 (IPEC-1, IPEC-J2)--spontaneously immortalised
Constanze Nossol, Anicò Barta-Böszörményi, Stefan Kahlert, Werner Zuschratter, Heidi Faber-Zuschratter, Nicole Reinhardt, Siriluk Ponsuksili, Klaus Wimmers, Anne-Kathrin Diesing, Hermann-Josef Rothkötter   +9 more
doaj   +1 more source

11S Glycinin Up-Regulated NLRP-3-Induced Pyroptosis by Triggering Reactive Oxygen Species in Porcine Intestinal Epithelial Cells

Frontiers in Veterinary Science, 2022
11S glycinin is a major soybean antigenic protein, which induces human and animal allergies. It has been reported to induce intestinal porcine epithelial (IPEC-J2) cell apoptosis, but the role of pyroptosis in 11S glycinin allergies remains unknown.
Lei Wang, Zhifeng Sun, Weina Xie, Chenglu Peng, Hongyan Ding, Yu Li, Shibin Feng, Xichun Wang, Chang Zhao, Jinjie Wu   +9 more
doaj   +1 more source

The tissue expression level of BPI gene in piglets from newborn to weaning and its relationship with Gram-negative bacterial infection

Journal of Integrative Agriculture, 2020
The bactericidal/permeability increasing protein (BPI) has an important function of nonspecific killing of Gram-negative bacteria. In this study, qPCR was used to detect the expression of the BPI gene in twelve tissues of Meishan piglets from birth to ...
Chao-hui DAI, Yue CAO, Zhong-cheng GAO, Guo-qiang ZHU, Sheng-long WU, Wen-bin BAO   +5 more
doaj   +1 more source

All-Trans Retinoic Acid Attenuates Transmissible Gastroenteritis Virus-Induced Apoptosis in IPEC-J2 Cells via Inhibiting ROS-Mediated P₃₈MAPK Signaling Pathway

Antioxidants, 2022
Transmissible gastroenteritis virus (TGEV) can cause diarrhea, dehydration, and high mortality in piglets, which is closely related to intestinal epithelial cell apoptosis caused by TGEV infection.
Junning Pu, Daiwen Chen, Gang Tian, Jun He, Zhiqing Huang, Ping Zheng, Xiangbing Mao, Jie Yu, Junqiu Luo, Yuheng Luo, Hui Yan, Bing Yu   +11 more
doaj   +1 more source

TGF-β from the Porcine Intestinal Cell Line IPEC-J2 Induced by Porcine Circovirus 2 Increases the Frequency of Treg Cells via the Activation of ERK (in CD4⁺ T Cells) and NF-κB (in IPEC-J2)

Viruses, 2022
Porcine circovirus 2 (PCV2) causes immunosuppression. Piglets infected with PCV2 can develop enteritis. Given that the gut is the largest immune organ, however, the response of the gut’s immune system to PCV2 is still unclear.
Xuewei Liu, Yang Wang, Cheng Han, Qiuming Li, Xiaolin Hou, Qinye Song, Shuanghai Zhou, Huanrong Li   +7 more
doaj   +1 more source