Results 1 to 10 of about 4,169 (171)

Dimethyl Itaconate Is Not Metabolized into Itaconate Intracellularly [PDF]

Journal of Biological Chemistry, 2017
Itaconic acid is an important metabolite produced by macrophages after stimulation with LPS. The role of itaconate in the inflammatory cascade is unclear. Here we used [13C]itaconate and dimethyl [13C]itaconate (DMI) to probe itaconate metabolism, and find that [13C]DMI is not metabolized to itaconate. [13C]Itaconate in the cell culture medium leads to
Charles Evans, Charles Burant, Brent Martin   +2 more
exaly   +3 more sources

Dimethyl itaconate induces long-term innate immune responses and confers protection against infection

Cell Reports, 2023
Summary: Itaconate is an immunomodulatory metabolite produced by immune cells under microbial stimulation and certain pro-inflammatory conditions and triggers antioxidant and anti-inflammatory responses.
László Groh
exaly   +3 more sources

4-octyl itaconate as a metabolite derivative inhibits inflammation via alkylation of STING

Cell Reports, 2023
Summary: The Krebs cycle-derived metabolite itaconate, whose production is catalyzed by immune response gene 1 (IRG1), has potential to link immunity and metabolism in activated macrophages through alkylation or competitive inhibition of target proteins.
Jiaqi Kang, Weizhen Li
exaly   +3 more sources

The signaling pathways and therapeutic potential of itaconate to alleviate inflammation and oxidative stress in inflammatory diseases

Redox Biology, 2022
Endogenous small molecules are metabolic regulators of cell function. Itaconate is a key molecule that accumulates in cells when the Krebs cycle is disrupted.
Juan Wei, Fu Li, Xin Lv
exaly   +3 more sources

Itaconate transport across the plasma membrane and Salmonella-containing vacuole via MCT1/4 modulates macrophage antibacterial activity [PDF]

Nature Communications
Itaconate accumulates in macrophages upon bacterial infection, and manifests antibacterial activity. Convincing evidence substantiates that itaconate is transported across the plasma membrane and vacuolar membrane, but the molecular bases underlying ...
Qingcai Meng, Chengxi Li, Yuping Cai, Ying Chen, Xiaoqing Chen, Xin Wang, Biling Zhang, Yue Zhang, Feng Liu, Meixin Chen   +9 more
doaj   +2 more sources

The cardiovascular risk marker itaconate is sex-dependently associated with legume intake and immune-inflammatory competence in subjects with high BMI [PDF]

Frontiers in Immunology
BackgroundItaconate has received attention as a key immunometabolic mediator produced by activated macrophages, linking cellular metabolism to inflammatory responses.
Amanda Cuevas-Sierra, Andrea Higuera-Gómez, Andrea Higuera-Gómez, Begoña de Cuevillas, Gabriela Paula-Buestan, María Martínez-Urbistondo, Raquel Castejón, J. Antonio Vargas, José Moisés Laparra, José Moisés Laparra, J. Alfredo Martínez, J. Alfredo Martínez, J. Alfredo Martínez   +12 more
doaj   +2 more sources

Protocol to study the role of endogenously produced itaconate using CRISPR-Cas9 technology in THP-1 cells [PDF]

STAR Protocols
Summary: Itaconate mimetics inadequately represent endogenous itaconate, a negative regulator of innate immune-driven pro-inflammatory cytokines. We present a CRISPR-Cas9 protocol to delete a 4-nucleotide region in the immunoresponsive gene 1 (IRG1 ...
Luke A. Bourner, Katie A. Acken, Haiyan Long, Linda A. Chung, Kenneth D. Roth, Frank C. Dorsey   +5 more
doaj   +2 more sources

Itaconate confers tolerance to late NLRP3 inflammasome activation

Cell Reports, 2021
Summary: Itaconate is a unique regulatory metabolite that is induced upon Toll-like receptor (TLR) stimulation in myeloid cells. Here, we demonstrate major inflammatory tolerance and cell death phenotypes associated with itaconate production in activated
Martina Kerndl, Denis Mogilenko, Gaya Amarasinghe   +2 more
exaly   +3 more sources

Itaconate and its derivatives ameliorate autoimmunity by suppressing Th17 cells via regulating mitophagy [PDF]

Cell Communication and Signaling
Itaconate (ITA) originates by decarboxylation of cis-aconitate catalyzed by the enzyme aconitate decarboxylase 1. It has immunomodulatory properties, but its role in T cells, especially CD4+ T cells, is mostly unexplored. Here, we examined the effects of
Fuli Li, Qing Huang, Naisheng Zheng, Wenyu Zhang, Shan Luo, Ruowen Wang, Thulasiram Bathini, Pengkai Sun, Xinjian Li, Xian Huang, Tomasz Maj   +10 more
doaj   +2 more sources

Endogenously produced itaconate negatively regulates innate-driven cytokine production and drives global ubiquitination in human macrophages

Cell Reports
Summary: A wide variety of electrophilic derivatives of itaconate, the Kreb’s cycle-derived metabolite, are immunomodulatory, yet these derivatives have overlapping and sometimes contradictory activities.
Frank Dorsey
exaly   +3 more sources