De novo assembly and single-molecule study of kinetochore-microtubule interactions [PDF]
Frontiers in Cell and Developmental BiologyKinetochores are essential molecular machines composed of dozens of protein subcomplexes that assemble onto specialized centromeric nucleosomes during every cell cycle prior to mitosis.
Joshua D. Larson +4 more
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Monopolin subunit Csm1 associates with MIND complex to establish monopolar attachment of sister kinetochores at meiosis I. [PDF]
PLoS Genetics, 2013 Sexually reproducing organisms halve their cellular ploidy during gametogenesis by undergoing a specialized form of cell division known as meiosis. During meiosis, a single round of DNA replication is followed by two rounds of nuclear divisions (referred
Sourav Sarkar +6 more
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The Art of Chromosome Capture: Kinetochore Structures Across Evolution. [PDF]
Structural DynamicsKinetochores are essential macromolecular machines that anchor chromosomes to the mitotic spindle, ensuring accurate chromosome segregation during cell division.
Stanislau Yatskevich
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Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint. [PDF]
PLoS ONE, 2009 BackgroundKinetochores attach sister chromatids to microtubules of the mitotic spindle and orchestrate chromosome disjunction at anaphase. Although S. cerevisiae has the simplest known kinetochores, they nonetheless contain approximately 70 subunits that
Cinzia Pagliuca +4 more
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MPS1 promotes timely spindle bipolarization to prevent kinetochore-microtubule attachment errors in oocytes [PDF]
The EMBO JournalIncorrect kinetochore–microtubule attachment leads to chromosome segregation errors. The risk of incorrect attachment is high in acentrosomal oocytes, where kinetochores are surrounded by randomly oriented microtubules until spindle bipolarization ...
Shuhei Yoshida +3 more
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Chromosome segregation in plant meiosis [PDF]
Frontiers in Plant Science, 2014 Faithful chromosome segregation in meiosis is essential for ploidy stability over sexual life cycles. In plants, defective chromosome segregation caused by gene mutations or other factors leads to the formation of unbalanced or unreduced gametes creating
Linda eZamariola +4 more
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Interplay of kinetochores and catalysts drives rapid assembly of the mitotic checkpoint complex [PDF]
Nature CommunicationsThe spindle assembly checkpoint (SAC) ensures mitotic exit occurs only after sister chromatid biorientation, but how this coordination is mechanistically achieved remains unclear.
Suruchi Sethi +6 more
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Functional characterization of the Csm1-like protein TITAN 9 in Arabidopsis thaliana [PDF]
iScienceSummary: Kinetochores are essential for chromosome segregation in eukaryotes. An important component of kinetochores in opisthokonta is Csm1. However, its function appears to be diversified and, while Csm1 in budding yeast is a component of the monopolin
Zhijian Zhang +8 more
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Effects of malleable kinetochore morphology on measurements of intrakinetochore tension [PDF]
Open Biology, 2020 The distance between fluorescent spots formed by various kinetochore proteins (delta) is commonly interpreted as a manifestation of intrakinetochore tension (IKT) caused by microtubule-mediated forces.
Fioranna Renda +6 more
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Cells, 2023 Nucleolar and Spindle-Associated Protein 1 (NuSAP1) is an important mitotic regulator, implicated in control of mitotic microtubule stability and chromosome segregation. NuSAP1 regulates these processes by interacting with several protein partners.
Michela Damizia +3 more
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