Results 21 to 30 of about 3,159 (153)

Bub3p facilitates spindle checkpoint silencing in fission yeast [PDF]

, 2009
Although critical for spindle checkpoint signaling, the role kinetochores play in anaphase promoting complex (APC) inhibition remains unclear. Here we show that spindle checkpoint proteins are severely depleted from unattached kinetochores in fission ...
Hardwick, Kevin G., Millar, Jonathan B.A., van der Sar, Sjaak J. A., van der Sar, Sjaak J A, van Der Sar, Sjaak J.A., Millar, Jonathan B. A., Vanoosthuyse, Vincent, Millar, Jonathan B A, Meadows, John C, Vanoosthuyse, V., Hardwick, Kevin, Meadows, John, Meadows, John C., Hardwick, Kevin G   +13 more
core   +1 more source

Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex [PDF]

, 2012
Kinetochores are multi-protein megadalton assemblies that are required for attachment of microtubules to centromeres and, in turn, the segregation of chromosomes in mitosis. Kinetochore assembly is a cell cycle regulated multi-step process.
Tobias Ulbricht, Deng, Wen, Hellwig Daniela, Hofmeister, Antje, Hellwig, Daniela, Ulbricht Tobias, Döring, Volker, Leonhardt, Heinrich, McAinsh, Andrew D., Wen Deng, Eskat, Anja, James M Bancroft, Emmerth Stephan, Bancroft, James M, Diekmann, Stephan, Bancroft, James M., Volker Döring, Döring Volker, Bancroft James M., Cardoso, M. Cristina, Anja Eskat, Leonhardt Heinrich, Antje Hofmeister, Stephan Emmerth, Christian Hoischen, Rudolphi, Sven, Emmerth, Stephan, Hoischen, Christian, Stephan Diekmann, Rudolphi Sven, Cardoso, M Cristina, Eskat Anja, Heinrich Leonhardt, Hoischen Christian, Sven Rudolphi, Cardoso M. Cristina, Diekmann Stephan, Deng Wen, M Cristina Cardoso, Ulbricht, Tobias, Hofmeister Antje, Meraldi Patrick, McAinsh, Andrew D, Patrick Meraldi, McAinsh Andrew D., Meraldi, Patrick, Daniela Hellwig, Andrew D McAinsh   +47 more
core   +1 more source

Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation [PDF]

, 2015
During mitosis, Bub1 kinase phosphorylates histone H2A-T120 to promote centromere sister chromatid cohesion through recruitment of shugoshin (Sgo) proteins. The regulation and dynamics of H2A-T120 phosphorylation are poorly understood. Using quantitative
Lajeunesse, Audrey, Asghar, Adeel, Combes, Guillaume, Thebault, Philippe, Dulla, Kalyan, Elowe, Sabine, Nigg, Erich A.   +6 more
core   +1 more source

Exotic mitotic mechanisms [PDF]

, 2012
The emergence of eukaryotes around two billion years ago provided new challenges for the chromosome segregation machineries: the physical separation of multiple large and linear chromosomes from the microtubule-organizing centres by the nuclear envelope.
Drechsler, Hauke, McAinsh, Andrew D.
core   +1 more source

A tripartite mechanism catalyzes Mad2-Cdc20 assembly at unattached kinetochores [PDF]

, 2021
During cell division, kinetochores couple chromosomes to spindle microtubules. To protect against chromosome gain or loss, kinetochores lacking microtubule attachment locally catalyze association of the checkpoint proteins Cdc20 and Mad2, which is the ...
Corbett, Kevin, Lara-Gonzalez, Pablo, Kim, Taekyung, Oegema, Karen, Desai, Arshad   +4 more
core   +1 more source

The ultrastructure of the kinetochore and kinetochore fiber in Drosophila somatic cells [PDF]

Chromosoma, 2006
Drosophila melanogaster is a widely used model organism for the molecular dissection of mitosis in animals. However, despite the popularity of this system, no studies have been published on the ultrastructure of Drosophila kinetochores and kinetochore fibers (K-fibers) in somatic cells.
Maiato, H, Hergert, PJ, Moutinho-Pereira, S, Dong, Y, VandenBeldt, KJ, Rieder, CL, McEwen, BF   +6 more
openaire   +3 more sources

Evidence of Zip1 Promoting Sister Kinetochore Mono-orientation During Meiosis in Budding Yeast

G3: Genes, Genomes, Genetics, 2018
Halving of the genome during meiosis I is achieved as the homologous chromosomes move to the opposite spindle poles whereas the sister chromatids stay together and move to the same pole.
Hemant Kumar Prajapati, Meenakshi Agarwal, Priyanka Mittal, Santanu K. Ghosh   +3 more
doaj   +1 more source

The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function [PDF]

, 2005
During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby preventing chromosome missegregation. A genetic screen
Vanoosthuyse, V., Hardwick, Kevin, Kadura, Sheila, Sazer, Shelley, He, Xiangwei, Vanoosthuyse, Vincent, Hardwick, Kevin G   +6 more
core   +1 more source

Checkpoint Proteins Bub1 and Bub3 Delay Anaphase Onset in Response to Low Tension Independent of Microtubule-Kinetochore Detachment

Cell Reports, 2019
Summary: The spindle assembly checkpoint (SAC) delays anaphase onset until sister chromosomes are bound to microtubules from opposite spindle poles. Only then can dynamic microtubules produce tension across sister kinetochores.
Kathleen G. Proudfoot, Samuel J. Anderson, Sandeep Dave, Angela R. Bunning, Pallavi Sinha Roy, Abesh Bera, Mohan L. Gupta, Jr.   +6 more
doaj   +1 more source

Evidence of Non-microtubule Spindle Forces in Mesostoma ehrenbergii Spermatocytes

Frontiers in Molecular Biosciences, 2020
We tested conclusions reached in previous experiments in which Mesostoma spermatocyte chromosomes moved rapidly to a pole in the absence of microtubules: after 10 μM nocodazole (NOC) depolymerized metaphase spindle microtubules, kinetochores from each of
Eleni Fegaras-Arch, Michael Berns, Michael Berns, Arthur Forer   +3 more
doaj   +1 more source