Liver organoid culture methods [PDF]
Cell & Bioscience, 2023 Organoids, three-dimensional structures cultured in vitro, can recapitulate the microenvironment, complex architecture, and cellular functions of in vivo organs or tissues.
Yiqing Hu +10 more
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Human liver organoid: modeling liver steatosis and beyond [PDF]
Cell Regeneration, 2023 Steatosis, as the early stage of nonalcoholic fatty acid disease (NAFLD), would progress into nonalcoholic steatohepatitis (NASH) and liver failure without intervention.
Jinsong Wei, Wen Zhang, Bing Zhao
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Emerging liver organoid platforms and technologies [PDF]
Cell Regeneration, 2021 Building human organs in a dish has been a long term goal of researchers in pursue of physiologically relevant models of human disease and for replacement of worn out and diseased organs.
Do Thuy Uyen Ha Lam +3 more
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Placenta-derived factors contribute to human iPSC-liver organoid growth [PDF]
Nature CommunicationsOrganoids derived from human induced pluripotent stem cells (hiPSC) are potentially applicable for regenerative medicine. However, the applications have been hampered by limited organoid size and function as a consequence of a lack of progenitor ...
Yoshiki Kuse +13 more
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Adult and iPS-derived non-parenchymal cells regulate liver organoid development through differential modulation of Wnt and TGF-β [PDF]
Stem Cell Research & Therapy, 2019 Background Liver organoid technology holds great promises to be used in large-scale population-based drug screening and in future regenerative medicine strategies.
Ernesto Goulart +12 more
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Long-Term Adult Feline Liver Organoid Cultures for Disease Modeling of Hepatic Steatosis [PDF]
Stem Cell Reports, 2017 Summary: Hepatic steatosis is a highly prevalent liver disease, yet research is hampered by the lack of tractable cellular and animal models. Steatosis also occurs in cats, where it can cause severe hepatic failure.
Hedwig S. Kruitwagen +20 more
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Integrated multi-omics, spatial profiling and organoid modeling drive transformative advances in chronic liver disease and hepatocellular carcinoma immunomicroenvironment research [PDF]
Frontiers in ImmunologyChronic liver disease (CLD) represents a major global public health challenge, necessitating a systematic understanding of its complex immunopathological mechanisms.
Qi Liu +9 more
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Construction of mouse liver aging model by hepatocyte organoid [PDF]
Jichu yixue yu linchuang, 2023 Objective Construction of mouse liver senescence model induced rapidly in vitro by 3D organoid culture. Methods Mouse hepatocytes were collected by two-step perfusion method and cultured in 3D.
LU Yan, ZHOU Yabo, CHEN Jie
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Bioengineering Liver Organoids for Diseases Modelling and Transplantation
Bioengineering, 2022 Organoids as three-dimension (3D) cellular organizations partially mimic the physiological functions and micro-architecture of native tissues and organs, holding great potential for clinical applications.
Junzhi Li +5 more
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Organoids to model liver diseaseKey points
JHEP Reports, 2021 Summary: The organoid model represents a major breakthrough in cell biology that has revolutionised biomedical research. Organoids are 3D physiological in vitro structures that recapitulate morphological and functional features of in vivo tissues and ...
Sandro Nuciforo, Markus H. Heim
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