LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival. [PDF]
, 2018 Epithelial dysfunction and crypt destruction are defining features of inflammatory bowel disease (IBD). However, current IBD therapies targeting epithelial dysfunction are lacking.
Bayrer, James R +8 more
core +2 more sources
Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation. [PDF]
, 2016 Adult somatic tissues have proven difficult to expand in vitro, largely because of the complexity of recreating appropriate environmental signals in culture.
AM Ingalls +43 more
core +11 more sources
Challenges for the Applications of Human Pluripotent Stem Cell-Derived Liver Organoids
Frontiers in Cell and Developmental Biology, 2021 The current organoid culture systems allow pluripotent and adult stem cells to self-organize to form three-dimensional (3D) structures that provide a faithful recapitulation of the architecture and function of in vivo organs.
Mingyang Chang +2 more
doaj +1 more source
Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo [PDF]
, 2017 While the use of bioluminescent proteins for molecular imaging is a powerful technology to further our understanding of complex processes, fluorescent labeling with visible light fluorescent proteins such as GFP and RFP suffers from poor tissue ...
Blyth, Karen +11 more
core +1 more source
Biliary Atresia – emerging diagnostic and therapy opportunities
EBioMedicine, 2021 Biliary Atresia is a devastating pediatric cholangiopathy affecting the bile ducts of the liver. In this review, we describe recent progress in the understanding of liver development with a focus on cholangiocyte differentiation and how use of technical ...
Urban Lendahl +3 more
doaj +1 more source
Organoids for the Study of Liver Cancer
Seminars in Liver Disease, 2021 AbstractLiver cancer is the second most lethal malignancy worldwide. Cell lines and murine models are the most common tools for modeling human liver carcinogenesis. Most recently, organoids with a three-dimensional structure derived from primary tissues or cells have been applied to liver cancer research.
Wang, Haichuan +2 more
openaire +5 more sources
Construction of 3D in vitro models by bioprinting human pluripotent stem cells: Challenges and opportunities [PDF]
, 2019 Three-dimensional (3D) printing of biological material, or 3D bioprinting, is a rapidly expanding field with interesting applications in tissue engineering and regenerative medicine. Bioprinters use cells and biocompatible materials as an ink (bioink) to
Rosa, Alessandro, Salaris, Federico
core +1 more source
Modeling inflammation and oxidative stress in gastrointestinal disease development using novel organotypic culture systems. [PDF]
, 2013 Gastroesophageal reflux disease (GERD), Barrett's esophagus (BE), graft-versus-host disease (GVHD), and inflammatory bowel diseases such as ulcerative colitis and Crohn's disease are common human gastrointestinal diseases that share inflammation as a key
Bortner, James D +6 more
core +2 more sources
Opportunities for organoids as new models of aging. [PDF]
, 2018 The biology of aging is challenging to study, particularly in humans. As a result, model organisms are used to approximate the physiological context of aging in humans. However, the best model organisms remain expensive and time-consuming to use.
Antonica +145 more
core +2 more sources
Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells
Cell Reports, 2017 Summary: Organoid technology provides a revolutionary paradigm toward therapy but has yet to be applied in humans, mainly because of reproducibility and scalability challenges.
Takanori Takebe +24 more
doaj +1 more source