Structural basis of complement membrane attack complex formation [PDF]
Nature Communications, 2016 The membrane attack complex (MAC) is an immune effector that kills pathogens by forming pores in their membrane. Here the authors use cryo-electron microscopy to reveal that the full MAC is an asymmetric pore with a split-washer configuration and ...
Marina Serna +3 more
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Soluble Membrane Attack Complex: Biochemistry and Immunobiology [PDF]
Frontiers in Immunology, 2020 The soluble membrane attack complex (sMAC, a.k.a., sC5b-9 or TCC) is generated on activation of complement and contains the complement proteins C5b, C6, C7, C8, C9 together with the regulatory proteins clusterin and/or vitronectin.
Scott R. Barnum +2 more
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Structural basis for membrane attack complex inhibition by CD59 [PDF]
Nature Communications, 2023 CD59 protects human cells from damage by the MAC immune pore. The authors show how CD59 inhibits MAC, by deflecting pore-forming β-hairpins of complement proteins.
Emma C. Couves +6 more
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In vitro Measurement of Membrane Attack Complex in RPE Cells [PDF]
Bio-Protocol, 2021 Initiation of the complement system results in the formation of a multiprotein pore termed the membrane attack complex (MAC, C5b-C9). MAC pores accumulate on a cell surface and can result in cell lysis.
Kelly Mulfaul, Sarah Doyle
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Structural basis of soluble membrane attack complex packaging for clearance [PDF]
Nature Communications, 2021 To prevent unregulated complement activation, extracellular chaperones capture soluble precursors to the membrane attack complex (sMAC). Here, structural analysis of sMAC reveals how clusterin recognizes heterogeneous sMAC complexes and inhibits ...
Anaïs Menny +5 more
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Structure of the poly-C9 component of the complement membrane attack complex [PDF]
Nature Communications, 2016 The membrane attack complex is a heteromeric assembly of complement proteins where multiple copies of C9 are recruited by the C5b678 complex to form lytic pores in pathogen membranes.
Natalya V. Dudkina +13 more
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Deposition of the Membrane Attack Complex in Healthy and Diseased Human Kidneys [PDF]
Frontiers in Immunology, 2021 The membrane attack complex—also known as C5b-9—is the end-product of the classical, lectin, and alternative complement pathways. It is thought to play an important role in the pathogenesis of various kidney diseases by causing cellular injury and tissue
Jacob J. E. Koopman +5 more
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Membrane Attack Complex Mediates Retinal Pigment Epithelium Cell Death in Stargardt Macular Degeneration [PDF]
Cells, 2022 Recessive Stargardt disease (STGD1) is an inherited retinopathy caused by mutations in the ABCA4 gene. The ABCA4 protein is a phospholipid-retinoid flippase in the outer segments of photoreceptors and the internal membranes of retinal pigment epithelial (
Eunice Sze Yin Ng +8 more
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The neoepitope of the complement C5b-9 Membrane Attack Complex is formed by proximity of adjacent ancillary regions of C9 [PDF]
Communications Biology, 2023 The cryo-EM structure of monoclonal antibody aE11 bound to polyC9 reveals that the binding site within the Membrane Attack Complex is a quaternary discontinuous epitope formed by two separate surfaces of the oligomeric C9 periphery.
Charles Bayly-Jones +11 more
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Quantitative Analysis of Complement Membrane Attack Complex Proteins Associated with Extracellular Vesicles [PDF]
ProteomesExtracellular vesicles (EVs) represent a universal mechanism of intercellular communication in normal and pathological conditions. There are reports showing the presence of complement proteins in EV preparations, specifically those that can form a ...
Illarion V. Turko
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