Results 241 to 250 of about 130,672 (286)

Mitotic Spindle Assembly In Vitro

Current Protocols in Cell Biology, 2001
AbstractThe protocols in this unit describe the preparation of materials for an in vitro assay of mitotic spindle assembly in Xenopus egg extracts. Fluorochrome‐labeled tubulin is used to visualize microtubule asters and spindles.
J, Merlie, R, Heald
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ATPases in Mitotic Spindles

1984
Publisher Summary This chapter focuses on one aspect of the biochemistry of mitotic spindles—the presence of adenosine triphosphate (ATP) hydrolyzing enzymes. ATPase in the mitotic spindle is discussed. There is a physiological evidence for dynein ATPase in mitotic spindles.
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Mitotic Spindle Dynamics in Drosophila

2007
Mitosis, the process by which the replicated chromosomes are segregated equally into daughter cells, has been studied for over a century. Drosophila melanogaster is an ideal organism for this research. Drosophila embryos are well suited to image mitosis, because during cycles 10-13 nuclei divide rapidly at the surface of the embryo, but mitotic cells ...
Ingrid, Brust-Mascher, Jonathan M, Scholey   +1 more
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Actin in the Mitotic Spindle

Nature, 1971
I PREVIOUSLY suggested that the shape of the metaphase spindle cannot be maintained if there is tension in the component microtubules1. Spindle tension has been detected2,3 and could be due to some other component pulling the chromosomes over the rigid microtubular framework.
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Sliding filaments and mitotic spindle organization

Nature Cell Biology, 2014
Mitosis depends upon the action of the mitotic spindle, a subcellular machine that uses microtubules (MTs) and motors to assemble itself and to coordinate chromosome segregation. Recent work illuminates how the motor-driven poleward sliding of MTs - nucleated at centrosomes, chromosomes and on pre-existing MTs - contributes to spindle assembly and ...
Haifeng, Wang, Ingrid, Brust-Mascher, Jonathan M, Scholey   +2 more
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GENETIC ANALYSIS OF THE MITOTIC SPINDLE

Annual Review of Genetics, 1996
▪ Abstract  Much of our understanding of the molecular basis of mitotic spindle function has been achieved within the past decade. Studies utilizing genetically tractable organisms have made important contributions to this field and these studies form the basis of this review.
M A, Hoyt, J R, Geiser
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Mescaline as a mitotic spindle inhibitor

Nature, 1976
COLCHICINE and its methyl derivative colcemid not only inhibit microtubule self-assembly1 but also affect fast transport in nerve2. A report that mescaline likewise inhibits the rapid component of axoplasmic transport in nerve3, led us to investigate the effects of this drug on mitosis, and on purified microtubule protein (tubulin4).
C M, Harrisson, B M, Page, H M, Keir
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C-microtubules in isolated mitotic spindles

Journal of Cell Science, 1971
ABSTRACT Microtubules with incomplete cylindrical structure are present in isolated mitotic spindles of the sea urchin, Arbacia punctulata. In cross-section they appear C-shaped, and are thus similar to the ‘C-microtubules ‘or ‘C-filaments’ observed previously in other systems.
W D, Cohen, T, Gottlieb
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Mitotic Spindle Assembly Mechanisms

2008
The mitotic spindle consists of dynamic microtubules and many associated factors that form an antiparallel, bipolar array. Duplicated chromosomes are attached to microtubules of the spindle and then are physically separated by the spindle to opposite ends of the dividing cell. Spindles vary in their morphology and assembly pathway depending on the cell
Rebecca Heald, Claire E. Walczak
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The Mitotic Spindle

Scientific American, 1989
J R, McIntosh, K L, McDonald
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