Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis [PDF]
, 2003 We have investigated the function of mitotic kinesin-like protein (MKlp) 2, a kinesin localized to the central spindle, and demonstrate that its depletion results in a failure of cleavage furrow ingression and cytokinesis, and disrupts localization of ...
Mayer, Thomas U. +13 more
core +1 more source
Fission yeast cells undergo nuclear division in the absence of spindle microtubules. [PDF]
PLoS Biology, 2010 Mitosis in eukaryotic cells employs spindle microtubules to drive accurate chromosome segregation at cell division. Cells lacking spindle microtubules arrest in mitosis due to a spindle checkpoint that delays mitotic progression until all chromosomes ...
Stefania Castagnetti +2 more
doaj +1 more source
Relocation of Aurora B from centromeres to the central spindle at the metaphase to anaphase transition requires MKlp2 [PDF]
, 2004 Mitotic kinases of the Polo and Aurora families are key regulators of chromosome segregation and cytokinesis. Here, we have investigated the role of MKlp1 and MKlp2, two vertebrate mitotic kinesins essential for cytokinesis, in the spatial regulation of ...
Erich A. Nigg +9 more
core +1 more source
Cell Division, 2019 Background At the onset of mitosis, the centrosome expands and matures, acquiring enhanced activities for microtubule nucleation and assembly of a functional bipolar mitotic spindle.
Chieh-Ting Fang +3 more
doaj +1 more source
Mechanisms and Molecules of the Mitotic Spindle [PDF]
Current Biology, 2004 In all eukaryotes, morphogenesis of the microtubule cytoskeleton into a bipolar spindle is required for the faithful transmission of the genome to the two daughter cells during division. This process is facilitated by the intrinsic polarity and dynamic properties of microtubules and involves many proteins that modulate microtubule organization and ...
Gadde, Sharat, Heald, Rebecca
openaire +2 more sources
Mitotic spindle proteomics in Chinese hamster ovary cells.
PLoS ONE, 2011 Mitosis is a fundamental process in the development of all organisms. The mitotic spindle guides the cell through mitosis as it mediates the segregation of chromosomes, the orientation of the cleavage furrow, and the progression of cell division.
Mary Kate Bonner +5 more
doaj +1 more source
Bub1 is a fission yeast kinetochore scaffold protein, and is sufficient to recruit other spindle checkpoint proteins to ectopic sites on chromosomes [PDF]
, 2007 The spindle checkpoint delays anaphase onset until all chromosomes have attached in a bi-polar manner to the mitotic spindle. Mad and Bub proteins are recruited to unattached kinetochores, and generate diffusible anaphase inhibitors.
Rischitor, Patricia E +8 more
core +2 more sources
Smurf2 as a novel mitotic regulator: From the spindle assembly checkpoint to tumorigenesis
Cell Division, 2009 The execution of the mitotic program with high fidelity is dependent upon precise spatiotemporal regulation of posttranslational protein modifications.
Moore Finola E +3 more
doaj +1 more source
Life Science Alliance, 2023 Using microinjection approaches in Drosophila embryos, Araújo et al reveal that acute disruption of the ER at centrosome-proximal sites perturbs the architecture of the mitotic spindle, with a direct impact on nuclear positioning.
Margarida Araújo +4 more
doaj +1 more source
Mitotic centromere-associated kinesin (MCAK) : a potential cancer drug target [PDF]
, 2011 The inability to faithfully segregate chromosomes in mitosis results in chromosome instability, a hallmark of solid tumors. Disruption of microtubule dynamics contributes highly to mitotic chromosome instability.
Yuan, Juping +4 more
core +1 more source