MLKL D144K mutation activates the necroptotic activity of the N-terminal MLKL domain [PDF]
, 2021 AbstractMixed-lineage kinase domain-like protein (MLKL) is an essential effector protein of necroptotic cell death. The four-helix bundle domain (4HB) presented by the first 125 amino acids of the N-terminal domain is sufficient for its necroptotic activity. However, it has been proposed that the subsequent helix H6 of the brace region has a regulatory
Katja Hrovat-Schaale +4 more
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Autophosphorylation at serine 166 regulates RIP kinase 1-mediated cell death and inflammation [PDF]
, 2020 Receptor interacting protein kinase 1 (RIPK1) regulates cell death and inflammatory responses downstream of TNFR1 and other receptors, and has been implicated in the pathogenesis of inflammatory and degenerative diseases.
Bertrand, Mathieu +11 more
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Sensing of endogenous nucleic acids by ZBP1 induces keratinocyte necroptosis and skin inflammation [PDF]
, 2020 Aberrant detection of endogenous nucleic acids by the immune system can cause inflammatory disease. The scaffold function of the signaling kinase RIPK1 limits spontaneous activation of the nucleic acid sensor ZBP1.
De Munck, Jolien +12 more
core +2 more sources
MLKL regulates necrotic plasma membrane permeabilization [PDF]
Cell Research, 2014 Recent data from two independent laboratories have shed new light on the molecular mechanisms by which mixed lineage kinase domain-like (MLKL) promotes a peculiar form of regulated necrosis known as necroptosis. Upon phosphorylation by receptor-interacting protein kinase 3 (RIPK3), MLKL appears indeed to form oligomers that localize to the plasma ...
Lorenzo, Galluzzi +2 more
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Staurosporine induces necroptotic cell death under caspase-compromised conditions in U937 cells [PDF]
, 2012 For a long time necrosis was thought to be an uncontrolled process but evidences recently have revealed that necrosis can also occur in a regulated manner.
Barna, Gabor +6 more
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Phosphorylated MLKL causes plasma membrane rupture [PDF]
Molecular & Cellular Oncology, 2014 Necroptosis, cell death caused by uncontrolled swelling (oncosis) and rapid plasma membrane rupture, exerts detrimental pathogenic effects.1,2 According to recent reports by Wang et al.3 in Molecul...
Linkermann, Andreas +2 more
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OncoImmunology, 2020 Vaccinia viruses (VACV) are a novel class of immune-oncolytic therapeutics and their mechanism of action is based both on their capacity to replicate selectively in cancer cells and to elicit danger signals that can boost anti-tumor immunity. We recently
Lien Van Hoecke +4 more
doaj +1 more source
Acylation of MLKL impacts its function in necroptosis
ACS Chemical Biology, 2023 ABSTRACTMixed lineage kinase domain-like (MLKL) is a key signaling protein of necroptosis. Upon activation by phosphorylation, MLKL translocates to the plasma membrane and induces membrane permeabilization which contributes to the necroptosis-associated inflammation.
Apoorva J. Pradhan +5 more
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MLKL-mediated necroptosis is a target for cardiac protection in mouse models of type-1 diabetes
Cardiovascular Diabetology, 2022 Background Cardiomyocyte death contributes to cardiac pathology of diabetes. Studies have shown that the RIPK3/MLKL necroptosis signaling is activated in diabetic hearts. Deletion of RIPK3 was reported to attenuate myocardial injury and heart dysfunction
Ting Cao +9 more
doaj +1 more source
Delivery of mixed-lineage kinase domain-like protein by vapor nanobubble photoporation induces necroptotic-like cell death in tumor cells [PDF]
, 2019 Modern molecular medicine demands techniques to efficiently deliver molecules directly into mammalian cells. As proteins are the final mediators of most cellular pathways, efficient intracellular protein delivery techniques are highly desired.
Braeckmans, Kevin +10 more
core +2 more sources