Results 21 to 30 of about 2,541,766 (304)

Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes

Nature Communications, 2017
The BioID approaches takes advantage of the promiscuous biotinylation enzyme (BirA*) to identify proteins that closely interact. Here the authors improve the resolution of BioID using a protein fragment complementation approach that allows the assignment
Isabel Myriam Schopp, Cinthia Claudia Amaya Ramirez, Jerneja Debeljak, Elisa Kreibich, Merle Skribbe, Klemens Wild, Julien Béthune   +6 more
doaj   +1 more source

Polyester materials for mRNA delivery

Exploration of Targeted Anti-tumor Therapy, 2022
Messenger RNA (mRNA) has recently made important progress in clinical implementation, offering a promising therapeutic option for infectious disease and cancer. However, the nature of mRNA molecules rendered them poorly bioavailable and unstable in vivo,
Wang Chen, Yonghui Ma, Xiaoxuan Liu, Dandan Zhu   +3 more
doaj   +1 more source

Integrative Analysis Of LncRNAs In Kidney Cancer To Discover A New LncRNA (LINC00847) As A Therapeutic Target For Staphylococcal Enterotoxin Tst Gene [PDF]

Cell Journal, 2020
Objective Bacterial toxin can cause cell death through induction of apoptosis in cancer cell lines as well as changes in the expression patterns of long non-coding RNAs (lncRNAs) and genes. In the present study, the effect of tst gene on ACHN cell lines
Maryam Safarpour-Dehkordi, Abbas Doosti, Mohammad-Saied Jami   +2 more
doaj   +1 more source

Active role of elongation factor G in maintaining the mRNA reading frame during translation. [PDF]

, 2019
During translation, the ribosome moves along the mRNA one codon at a time with the help of elongation factor G (EF-G). Spontaneous changes in the translational reading frame are extremely rare, yet how the precise triplet-wise step is maintained is not ...
Belardinelli, R., Bock, L., Grubmüller, H., Peng, B., Peske, F., Rodnina, M.   +5 more
core   +2 more sources

Gene Expression and the Physiological Role of Transforming Growth Factor-α in the Mouse Pituitary [PDF]

, 2003
Transforming growth factor-alpha (TGF-alpha), a member of the epidermal growth factor (EGF) family, is produced within the mouse anterior pituitaries. However, the cell types of TGF-alpha-expressing cells and the physiological roles of TGF-a within mouse
Kakeya, Tomoshi, Masui, Tohru, Oomizu, Souichi, Sharma, Seema, Takahashi, Sumio, Takeuchi, Sakae   +5 more
core   +1 more source

Ornithine Decarboxylase mRNA is Stabilized in an mTORC1-dependent Manner in Ras-transformed Cells [PDF]

, 2012
Upon Ras activation, ODC (ornithine decarboxylase) is markedly induced, and numerous studies suggest that ODC expression is controlled by Ras effector pathways. ODC is therefore a potential target in the treatment and prevention of Ras-driven tumours. In
Albig, Barreau, Bhattacharyya, Carracedo, Eulalio, Feith, Feith, Foat, Garneau, Gerner, Grens, Ito, Jacinto, Jian-Ying Wang, Johannes, Kashiwagi, Katsanou, Kawai, Kawai, Kedersha, Kertesz, Lai, Lal, Lan Xiao, Liao, Lisa M. Shantz, Liu, Lorenzini, Manzella, Marderosian, Mazan-Mamczarz, Nowotarski, Origanti, Proud, Rao, Repasky, Rousseau, Sarbassov, Shannon L. Nowotarski, Shantz, Shantz, Sofia Origanti, Song, Suswam, Suzanne Sass-Kuhn, Svitkin, Theresa D. Carr, Tong, Topisirovic, Zhang, Zou, Zou   +51 more
core   +2 more sources

Investigation of the anti-influenza activity of siRNA complexes against the cellular genes FLT4, Nup98, and Nup205 in vitro

Тонкие химические технологии, 2022
Objectives. Evaluation of changes in the viral activity of influenza A/WSN/33 after complex knockdown of combinations of cellular genes FLT4, Nup98 and Nup205 in human lung cell culture A549. Methods.
E. A. Pashkov, M. O. Korotysheva, A. V. Pak, E. B. Faizuloev, A. V. Sidorov, A. V. Poddubikov, E. P. Bystritskaya, Yu. E. Dronina, V. K. Solntseva, T. A. Zaiceva, E. P. Pashkov, A. S. Bykov, O. A. Svitich, V. V. Zverev   +13 more
doaj   +1 more source

Oxygen-dependent expression of the erythropoietin gene in rat hepatocytes in vitro [PDF]

, 1993
Since in juvenile rats the liver is the predominant site of erythropoietin (EPO) gene expression, we have used primary cultures of juvenile rat hepatocytes to establish and in vitro system for investigation of oxygen-dependent EPO formation.
Eckardt, Kai-Uwe, Kurtz, Armin, Pugh, C. W., Ratcliffe, P. J.   +3 more
core   +1 more source

Not so cold! Improving the thermostability of mRNA vaccines

Expert Review of Vaccines
Introduction One of the biggest challenges in the mRNA-LNP vaccine field is product stabilization to overcome the logistical hurdles linked to the ultra-cold distribution chain associated with first-generation mRNA SARS-CoV-2 vaccines.
Jean Haensler, Luc Even, Pierre Wils, Fethi Bensaid, Anusha Dias, Hongfeng Deng, Shrirang Karve, Frank DeRosa   +7 more
doaj   +1 more source

Association of clinical and epidemiological characteristics with COVID-19 BNT162b2 mRNA vaccine short-term adverse reactions in healthcare workers

Human Vaccines & Immunotherapeutics, 2021
Introduction The aim of the study was to investigate the prevalence and severity of adverse reactions (ARs) after immunization of healthcare workers (HCWs) with BNT162b2 vaccine and to associate them with clinical and epidemiological characteristics ...
Filippos Filippatos, Elizabeth-Barbara Tatsi, Charilaos Dellis, Nick Dessypris, Vassiliki Syriopoulou, Athanasios Michos   +5 more
doaj   +1 more source