Codon deoptimization of multispecific biologics reduces mispairing during transient mammalian protein expression [PDF]
Frontiers in Bioengineering and BiotechnologyIntroductionCodon optimization is utilized in biologics design to maximize protein expression. Selecting the host organism’s most frequently used codons for each amino acid can significantly enhance recombinant protein expression yields.
Timothy Z. Chang +10 more
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Toward Drug-Like Multispecific Antibodies by Design [PDF]
International Journal of Molecular Sciences, 2020 The success of antibody therapeutics is strongly influenced by their multifunctional nature that couples antigen recognition mediated by their variable regions with effector functions and half-life extension mediated by a subset of their constant regions.
Sawant, Manali S. +3 more
openaire +3 more sources
Antineutrophil Cytoplasmic Autoantibodies Specific to Bactericidal/Permeability-Increasing Protein: A Cross-Road Between Prolonged Gram-Negative Bacterial Infections and Ulcerative Colitis/Primary Sclerosing Cholangitis [PDF]
DiagnosticsBackground/Objectives: Binding of bactericidal/permeability-increasing (BPI) protein to Gram-negative (GN) bacteria plays a major role in bacterial elimination.
Dragana Jovanovic +7 more
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PLoS ONE, 2014 Multispecific proteins play a major role in controlling various functions such as signaling, regulation of transcription/translation, and immune response. Hence, a thorough understanding of the atomic-level principles governing multispecific interactions
Oz Sharabi +5 more
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Immunotherapy 3.0: Breakthroughs Steering the Next Generation of Cancer Control. [PDF]
Cancer Rep (Hoboken)ABSTRACT Background Hematologic disorders, including sickle cell disease, anemias, hemoglobinopathies, leukemias, lymphomas, and bleeding disorders, constitute a substantial and growing burden of morbidity and mortality across Africa. Structural health system constraints—limited diagnostic capacity, shortages of trained hematology specialists ...
Obeagu EI.
europepmc +2 more sources
Next generation Fc scaffold for multispecific antibodies [PDF]
iScience, 2021 Summary: Bispecific antibodies (Bispecifics) demonstrate exceptional clinical potential to address some of the most complex diseases. However, Bispecific production in a single cell often requires the correct pairing of multiple polypeptide chains for ...
Bram Estes +11 more
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“Stapling” scFv for multispecific biotherapeutics of superior properties
mAbs, 2023 Single-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen receptor T cells or natural killer cells.
Lauren E. Boucher +29 more
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Tradeoff between stability and multispecificity in the design of promiscuous proteins. [PDF]
PLoS Computational Biology, 2009 Natural proteins often partake in several highly specific protein-protein interactions. They are thus subject to multiple opposing forces during evolutionary selection.
Menachem Fromer, Julia M Shifman
doaj +4 more sources
Broadly Protective Antibody-Like Vaccines Against Highly Pathogenic Coronaviruses. [PDF]
J Med VirolABSTRACT SARS‐CoV‐2 and MERS‐CoV are highly pathogenic and contagious coronaviruses. Despite intensive vaccination, SARS‐CoV‐2 continues to spread, and no FDA‐approved vaccines exist for MERS‐CoV; thus, more effective and safer vaccine options are needed. The receptor‐binding domain (RBD) of coronaviruses is a primary target of neutralizing antibodies (
Helal A +20 more
europepmc +2 more sources
DNA‐Mediated Assembly of Multispecific Antibodies for T Cell Engaging and Tumor Killing
Advanced Science, 2020 Targeting T‐cells against cancer cells is a direct means of treating cancer, and has already shown great responses in clinical treatment of B‐cell malignancies.
Liqiang Pan, James J Chou
exaly +2 more sources