Nested PCR as a superior diagnostic method for Acanthamoeba keratitis compared to conventional techniques [PDF]
Scientific ReportsAcanthamoeba keratitis (AK) is a severe eye infection that can cause severe vision loss, especially in contact lens wearers. Rapid and accurate diagnosis is crucial for achieving favorable outcomes. Traditional methods, such as culture, are slow and have
Sarah Khaksar +13 more
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Comparative Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Human Brucellosis [PDF]
Archives of Razi Institute, 2021 Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective in the achievement of satisfying treatment results and prevention of chronic infections.
L Rahbarnia +3 more
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Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples [PDF]
Revista Brasileira de Parasitologia Veterinária, 2022 The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds.
Bruna Nicoleti Santana +4 more
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Single-Tube Nested PCR Using Immobilized Internal Primers [PDF]
BioTechniques, 2002 F.G.C. Abath +5 more
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Nested PCR Biases in Interpreting Microbial Community Structure in 16S rRNA Gene Sequence Datasets. [PDF]
PLoS ONE, 2015 Sequencing of the PCR-amplified 16S rRNA gene has become a common approach to microbial community investigations in the fields of human health and environmental sciences.
Guoqin Yu +4 more
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Leptospirosis diagnosis using Nested-PCR [PDF]
Brazilian Journal of Microbiology, 2003 Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods.
Nassi, Fernanda +7 more
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Semi-nested PCR para a detecção molecular de Paracoccidioides brasiliensis em amostras de tecido [PDF]
, 2010 INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Dionízio Filho, Pedro Sebastião Raimundo +5 more
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Nested PCR for Diagnosis of Typhoid Fever [PDF]
Journal of Clinical Microbiology, 2005 The superiority of the nested PCR employing H 1-d primers over culture or single-dilution Widal (4) should be reevaluated for isolates from unusual sites and from among those coinfected with human immunodeficiency virus (HIV)/AIDS. Numerous extraintestinal complications are known with Salmonella enterica serovar Typhi infection.
Subhash C, Arya +4 more
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Avian malaria is absent in juvenile colonial herons (Ardeidae) but not Culex pipiens mosquitoes in the Camargue, Southern France [PDF]
, 2015 Apicomplexan blood parasites Plasmodium and Haemoproteus (together termed “Avian malaria”) and Leucocytozoon are widespread, diverse vector-transmitted blood parasites of birds, and conditions associated with colonial nesting in herons (Ardeidae) and ...
Gauthier-Clerc, Michel +1 more
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Research in Plant Disease, 2015 The specific and sensitive nested-PCR method to detect Acidovorax citrulli, a causal agent of bacterial fruit blotch on cucurbitaceae, was developed. PCR primers were designed from the draft genome sequence which was obtained with the Next Generation ...
Young-Tak Kim +4 more
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