Nested PCR as a superior diagnostic method for Acanthamoeba keratitis compared to conventional techniques [PDF]
Scientific ReportsAcanthamoeba keratitis (AK) is a severe eye infection that can cause severe vision loss, especially in contact lens wearers. Rapid and accurate diagnosis is crucial for achieving favorable outcomes. Traditional methods, such as culture, are slow and have
Sarah Khaksar +13 more
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Comparison of Immunomagnetic Bead Separation-Immunosensor Detection and Nested-PCR Methods for Detecting Mycobacterium avium Subspecies paratuberculosis in Cattle Feces. [PDF]
J Clin Lab AnalKhosravi M +5 more
europepmc +3 more sources
Comparative Assessment of Diagnostic Performance of Cytochrome Oxidase Multiplex PCR and 18S rRNA Nested PCR. [PDF]
Korean J Parasitol, 2022 Kumari P +9 more
europepmc +3 more sources
Comparative Evaluation of LAMP and Nested PCR for the Rapid Diagnosis of Mycobacterium marinum Infection. [PDF]
Infect Drug Resist, 2023 Feng Y +5 more
europepmc +3 more sources
Comparative Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Human Brucellosis [PDF]
Archives of Razi Institute, 2021 Brucellosis is recognized as a zoonotic disease with high morbidity in the absence of treatment. The primary diagnosis of brucellosis can be effective in the achievement of satisfying treatment results and prevention of chronic infections.
L Rahbarnia +3 more
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Validation of a one-tube nested real-time PCR assay for the detection of Cryptosporidium spp. in avian fecal samples [PDF]
Revista Brasileira de Parasitologia Veterinária, 2022 The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds.
Bruna Nicoleti Santana +4 more
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Single-Tube Nested PCR Using Immobilized Internal Primers [PDF]
BioTechniques, 2002 F.G.C. Abath +5 more
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Nested PCR Biases in Interpreting Microbial Community Structure in 16S rRNA Gene Sequence Datasets. [PDF]
PLoS ONE, 2015 Sequencing of the PCR-amplified 16S rRNA gene has become a common approach to microbial community investigations in the fields of human health and environmental sciences.
Guoqin Yu +4 more
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Leptospirosis diagnosis using Nested-PCR [PDF]
Brazilian Journal of Microbiology, 2003 Leptospirosis is a worldwide sanitary problem. Its clinical signs resemble that of other diseases like Dengue and Flu, and it is difficult to distinguish between them. Currently available diagnostic methods shown low sensitivity and specificity. Efforts have been made to develop simpler, faster and more efficient diagnostic methods.
Nassi, Fernanda +7 more
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Semi-nested PCR para a detecção molecular de Paracoccidioides brasiliensis em amostras de tecido [PDF]
, 2010 INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Dionízio Filho, Pedro Sebastião Raimundo +5 more
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