Results 91 to 100 of about 137,602 (289)

Comparison of PCR-DGGE and Nested-PCR-DGGE Approach for Ammonia Oxidizers Monitoring in Membrane Bioreactors’ Activated Sludge

Archives of Environmental Protection, 2014
Nitritation, the first stage of ammonia removal process is known to be limiting for total process performance. Ammonia oxidizing bacteria (AOB) which perform this process are obligatory activated sludge habitants, a mixture consisting of Bacteria ...
Ziembińska-Buczyńska Aleksandra, Wiszniowski Jarosław, Ciesielski Sławomir   +2 more
doaj   +1 more source

Chagas disease reactivation in a heart transplant patient infected by domestic Trypanosoma cruzi discrete typing unit I (TcIDOM) [PDF]

, 2015
Background Trypanosoma cruzi, causative agent of Chagas disease, displays high intraspecific genetic diversity: six genetic lineages or discrete typing units (DTUs) are currently recognized, termed TcI through TcVI.
Bhattacharyya, T., Burleigh, B.A., Costales, J.A., Garcia-Perez, J., Kotton, C.N., Llewellyn, M.S., Messenger, L.A., Zurita-Leal, A.C.   +7 more
core   +2 more sources

TrxR2 Lactylation Facilitates Mitochondrial Protection and Endothelial Ferroptosis Resistance in Diabetic Cardiomyopathy

Advanced Science, EarlyView.
TrxR2 deletion in diabetic mice suppresses TUFM‐AMPK‐FUNDC1‐dependent mitophagy in endothelial cells, resulting in SCP2 upregulation and mitochondrial translocation of ACSL4. Mitochondrial ACSL4 promotes mitochondrial eicosanoid biosynthesis and ferroptosis, thereby aggravating cardiac microvascular injury and diabetic cardiomyopathy.
Su Li, Muyin Liu, Chao Chen, Xinyan Li, Xiaopei Yan, Wentao Zhu, Wenyan Qiu, Qiyu Li, Xiangyu Sun, Chao Huang, Ming Yin, Zhangwei Chen, Yao Lu, Junbo Ge, Xiangqing Kong, Juying Qian, Yuqiong Chen   +16 more
wiley   +1 more source

Nested-PCR do gene que codifica o antígeno b aplicada ao diagnóstico da tuberculose pulmonar Nested-PCR for gene that encodes the antigen b applied to the diagnosis of pulmonary tuberculosis

Revista da Sociedade Brasileira de Medicina Tropical, 2007
A reação em cadeia da polimerase usada para amplificação de uma seqüência interna de um fragmento previamente amplificado (nested-PCR) foi investigada como uma alternativa complementar a pesquisa de bacilos álcool ácido resistentes e a cultura do ...
Karla Valéria Batista Lima, Maria Luíza Lopes, Edvaldo Carlos Brito Loureiro, Maurimélia Mesquita Costa, Ninarosa Calzavara Cardoso, George Leandro Ferreira Lima, Maísa Silva Sousa   +6 more
doaj   +1 more source

Application of high-throughput sequencing to whole rabies viral genome characterisation and its use for phylogenetic re-evaluation of a raccoon strain incursion into the province of Ontario [PDF]

, 2017
Raccoon rabies remains a serious public health problem throughout much of the eastern seaboard of North America due to the urban nature of the reservoir host and the many challenges inherent in multi-jurisdictional efforts to administer co-ordinated and ...
Biek, Roman, Colville, Adam, Nadin-Davis, Susan A., Real, Leslie, Trewby, Hannah   +4 more
core   +1 more source

Detachment‐Induced FAK‐STAT3‐NNMT Inhibits CTCs Anoikis to Promote Breast Cancer Metastasis by Enhancing Fatty Acid Oxidation

Advanced Science, EarlyView.
The FAK‐STAT3‐NNMT axis drives anoikis resistance in circulating tumor cells by reprogramming fatty acid oxidation. Targeting this metabolic vulnerability suppresses metastasis, untangling a key mechanism of breast cancer progression and revealing NNMT as a promising therapeutic target.
Qingchao Tong, Yilei Ma, Yuzhen Gao, Lin Zhou, Luping Lou, Qiang Fang, Sining Fang, Ru Zhao, Jin Zeng, Jun Yang, Guoli Li, Xinyou Xie, Yanzhong Wang, Jun Zhang   +13 more
wiley   +1 more source

Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR Pesquisa de Neospora caninum em fetos bovinos por histologia, imunoistoquímica e nested-PCR

Revista Brasileira de Parasitologia Veterinária, 2009
Neospora caninum, a cause of abortion and stillbirth in cattle, was studied by histology, immunohistochemistry, and nested-PCR, using primers from the Nc5 region of the genomic DNA (PCR PLUS) and primers from the ITS1 region of the ribosomal DNA (PCR JB).
Aline Diniz Cabral, Clarice Neves Camargo, Nara Thiers Cacciatori Galleti, Liria Hiromi Okuda, Edviges Maristela Pituco, Claudia Del Fava   +5 more
doaj   +1 more source

Ehrlichia ruminantium detection using efficient high-throughput molecular method. [0088] [PDF]

, 2017
In order to improve sample screening capacity and E. ruminantium molecular diagnostics, an automated DNA extraction method for Amblyomma ticks and a new qPCR targeting the pCS20 gene region were successfully developed.
Albina, Emmanuel, Bournez, Laure, Cangi, Nidia, Lefrançois, Thierry, Neves, Luis, Pinarello, Valérie, Vachiéry, Nathalie   +6 more
core  

Ovine pedomics : the first study of the ovine foot 16S rRNA-based microbiome [PDF]

, 2011
We report the first study of the bacterial microbiome of ovine interdigital skin based on 16S rRNA by pyrosequencing and conventional cloning with Sanger-sequencing.
A Dhawi, AL Cogen, AP Escayg, Atiya Ul-Hassan, BF Langworth, BH Jost, Brian B Oakley, C Lozupone, Claire L Russell, DA Murdoch, DJ Lane, DJ Stewart, DL Emery, DS Roberts, E Pruesse, E Zaura, EA Grice, EA Grice, Elizabeth MH Wellington, G Bennett, G Conrads, G Jones, G Muyzer, G Sayers, GC Baker, GR Carter, Graham F Medley, H Ulbegi-Mohyla, H Zhou, I Demirkan, IH Davies, J Kaler, J Kaler, J Kaler, JA Frank, Jasmeet Kaler, JC Chin, JP Galkiewicz, JR Egerton, K Klitgaard, Laura E Green, LE Green, Leo A Calvo-Bado, LJ Moore, Luci Witcomb, M Chrino-Trejo, MC Liu, Miloslav Kocur, NJ Evans, NJ Evans, PA Lawson, PD Schloss, PD Schloss, RD Naylor, RE Ley, RJ Collighan, Rose Grogono-Thomas, RS Howell-Jones, S La Fontaine, S Lavín, S Piriz Duran, S Tadepalli, Scot E Dowd, SE Dowd, SJ Billington, TG Nagaraja, TM Skerman, TZ DeSantis, TZ DeSantis Jr, Vicky Bateman, W Li, W Ludwing, WE Kloos, WG Weisburg, WIB Beveridge, William Gaze   +75 more
core   +2 more sources

Versatile DNA Hydrogel‐Mediated Delivery of Ginsenoside‐Encapsulated Small Extracellular Vesicles to Boost Diabetic Wound Repair

Advanced Science, EarlyView.
This study presents a DNA hydrogel‐mediated delivery system, in which ginsenoside (GS) molecules are incorporated into small extracellular vesicles (sEV) secreted by mesenchymal stem cells (MSCs) and the formed complexes are then anchored in DNA hydrogels via aptamer‐CD63 affinity as “GS/sEV@DNAgels”, to improve diabetic wound repair.
Jianming Xing, Shuangyang Li, Yuning Wang, Xushuang Jia, Ruiting Lin, Xintong Liu, Dongxu Wang, Ning Cui, Peng Ji, Jiaqi Chen, Shengnian Wang, Guangzhe Li, Ye Teng, Da Liu, Ye Jin   +14 more
wiley   +1 more source