Nested PCR Analysis of Plasmodium Parasites [PDF]
, 2003 In the natural and untreated intermediate hosts, Plasmodium infections are maintained for extended and often lifelong periods. Parasitemia generally reaches its highest level in the first peak after inoculation, and only rises thereafter during increasingly brief and interspersed episodes. As the host acquires tolerance to higher numbers of multiplying
Snounou, Georges, Balbir, Singh
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Presence of multiple bacterial markers in clinical samples might be useful for presumptive diagnosis of infection in cirrhotic patients with culture-negative reports [PDF]
, 2016 Bacterial infections in cirrhotic patients with ascites are associated with a severe prognosis and an increased risk of death. The microbiological standard tests for the diagnosis of suspected infection, based on culture test of blood and ascitic fluid ...
Boaretti, M +4 more
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Nested PCR Assay for Detection of Granulocytic Ehrlichiae [PDF]
Journal of Clinical Microbiology, 1998 ABSTRACT A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood and serum samples obtained from seven humans with clinical presentations compatible with human granulocytic ehrlichiosis.
Massung, Robert F. +6 more
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Human papillomavirus detection and typing using a nested-PCR-RFLP assay
Brazilian Journal of Infectious Diseases, 2011 Background: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. Objectives: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, MSc +4 more
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An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection
Revista da Sociedade Brasileira de Medicina Tropical, 2013 Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and
Amanda de Faveri Pitz +6 more
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Frontiers in Veterinary Science, 2020 Porcine cytomegalovirus (PCMV) is a pathogen that must be removed from pigs for use as organ donors in xenotransplantation. Recently, it has been found that when donor pigs are infected with PCMV, a pig-to-non-human-primate xenotransplantation lower ...
Hye-young Wang +4 more
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Distribution and prevalence of malaria parasites among long-tailed macaques (Macaca fascicularis) in regional populations across Southeast Asia. [PDF]
, 2016 BackgroundPlasmodium knowlesi and Plasmodium cynomolgi are two malaria parasites naturally transmissible between humans and wild macaque through mosquito vectors, while Plasmodium inui can be experimentally transmitted from macaques to humans.
Du, Hongli +7 more
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Molecular confirmation of Sarcocystis fayeri in a donkey [PDF]
, 2017 Sarcocystis fayeri is a canine protozoan parasite with an equine intermediate host. Historically classified as an incidental pathogen, recent literature has described the toxic effects of Sarcocystis fayeri in human food poisoning, and highlighted ...
Coultous, Robert M. +4 more
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Comparison between four laboratory tests for routine diagnosis of enzootic bovine leukosis [PDF]
Kafkas Universitesi Veteriner Fakültesi Dergisi, 2022 The aim of this study was to compare the diagnostic capabilities of the agar gel immunodiff usion test (AGID) and two types of PCR, nested PCR (with nucleic acid extraction from blood) and direct blood nested PCR (db nested PCR) - without extraction, vs.
Nikolina RUSENOVA +2 more
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Rapid microarray-based method for monitoring of all currently known single-nucleotide polymorphisms associated with parasite resistance to antimalaria drugs. [PDF]
, 2007 Parasite drug resistance is partly conferred by single-nucleotide polymorphisms (SNPs), and monitoring them has been proposed as an alternative to monitoring drug resistance.
Crameri, Andreas +13 more
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