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Human Infections with Plasmodium knowlesi, the Philippines. [PDF]
, 2008 Five human cases of infection with the simian malaria parasite Plasmodium knowlesi from Palawan, the Philippines, were confirmed by nested PCR. This study suggests that this zoonotic infection is found across a relatively wide area in Palawan and ...
Bell, David +9 more
core +3 more sources
Nested PCR for Diagnosis of Typhoid Fever [PDF]
Journal of Clinical Microbiology, 2005 The superiority of the nested PCR employing H 1-d primers over culture or single-dilution Widal (4) should be reevaluated for isolates from unusual sites and from among those coinfected with human immunodeficiency virus (HIV)/AIDS. Numerous extraintestinal complications are known with Salmonella enterica serovar Typhi infection.
Subhash C, Arya +4 more
openaire +2 more sources
Research in Plant Disease, 2015 The specific and sensitive nested-PCR method to detect Acidovorax citrulli, a causal agent of bacterial fruit blotch on cucurbitaceae, was developed. PCR primers were designed from the draft genome sequence which was obtained with the Next Generation ...
Young-Tak Kim +4 more
doaj +1 more source
Presence of multiple bacterial markers in clinical samples might be useful for presumptive diagnosis of infection in cirrhotic patients with culture-negative reports [PDF]
, 2016 Bacterial infections in cirrhotic patients with ascites are associated with a severe prognosis and an increased risk of death. The microbiological standard tests for the diagnosis of suspected infection, based on culture test of blood and ascitic fluid ...
Boaretti, M +4 more
core +1 more source
Nested PCR Analysis of Plasmodium Parasites [PDF]
, 2003 In the natural and untreated intermediate hosts, Plasmodium infections are maintained for extended and often lifelong periods. Parasitemia generally reaches its highest level in the first peak after inoculation, and only rises thereafter during increasingly brief and interspersed episodes. As the host acquires tolerance to higher numbers of multiplying
Snounou, Georges, Balbir, Singh
openaire +2 more sources
Avian malaria is absent in juvenile colonial herons (Ardeidae) but not Culex pipiens mosquitoes in the Camargue, Southern France [PDF]
, 2015 Apicomplexan blood parasites Plasmodium and Haemoproteus (together termed “Avian malaria”) and Leucocytozoon are widespread, diverse vector-transmitted blood parasites of birds, and conditions associated with colonial nesting in herons (Ardeidae) and ...
Gauthier-Clerc, Michel +1 more
core +5 more sources
Nested PCR Assay for Detection of Granulocytic Ehrlichiae [PDF]
Journal of Clinical Microbiology, 1998 ABSTRACT A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood and serum samples obtained from seven humans with clinical presentations compatible with human granulocytic ehrlichiosis.
Massung, Robert F. +6 more
openaire +2 more sources
Human papillomavirus detection and typing using a nested-PCR-RFLP assay
Brazilian Journal of Infectious Diseases, 2011 Background: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. Objectives: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, MSc +4 more
doaj +1 more source
An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection
Revista da Sociedade Brasileira de Medicina Tropical, 2013 Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and
Amanda de Faveri Pitz +6 more
doaj +1 more source
Frontiers in Veterinary Science, 2020 Porcine cytomegalovirus (PCMV) is a pathogen that must be removed from pigs for use as organ donors in xenotransplantation. Recently, it has been found that when donor pigs are infected with PCMV, a pig-to-non-human-primate xenotransplantation lower ...
Hye-young Wang +4 more
doaj +1 more source