Results 21 to 30 of about 137,602 (289)

A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples Semi-nested PCR para a detecção molecular de Paracoccidioides brasiliensis em amostras de tecido

Revista da Sociedade Brasileira de Medicina Tropical, 2010
INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Andrea Cristine Koishi, Débora Fonseca Vituri, Pedro Sebastião Raimundo Dionízio Filho, Alexandre Augusto Sasaki, Maria Sueli Soares Felipe, Emerson José Venancio   +5 more
doaj   +1 more source

Efficiency of Rz-1 based rhizomania resistance and molecular studies on BNYVV isolates from sugar beet cultivation in Geece [PDF]

, 2011
A survey was carried out to investigate the current situation concerning rhizomania disease incidence in sugar beet cultivation of Greece. A systematic field evaluation over locations and years revealed a consistent disease severity pattern according to ...
Goldbach, R.W., Pavli, R., Prins, M.W., Skaracis, G.N.   +3 more
core   +2 more sources

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa, C.N. Nozaki, N.S.C. Lira, J.M.A.P. Antunes, M.N. Xavier, E.A. Costa, T.A. Paixão, R.L. Santos, J. Megid   +8 more
doaj   +1 more source

Tracking of Coetaneous Leishmaniasis by Parasitological, Molecular and Biochemical Analysis

Kufa Journal for Nursing Sciences, 2015
Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal ...
Sundus Nsaif AL-Hucheimi, Baqur A. Sultan, Muhsin A. Al-Dhalimi, Thikra A. Mahmood   +3 more
doaj   +1 more source

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients

BMC Infectious Diseases, 2004
Background Molecular detection of herpesviruses DNA is considered as the reference standard assay for diagnosis of central nervous system infections. In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV ...
Bartolone Rosaria, Giuliani Giuseppe, De Vecchi Elena, Lombardi Alessandra, Drago Lorenzo, Gismondo Maria   +5 more
doaj   +1 more source

Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]

Veterinary World, 2017
Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal, Sunit Kumar Mukhopadhayay, Saktipada Pradhan, Samiran Mondal, Chandrakanta Jana, Nimai Chandra Patra, Rabindra Nath Hansda   +6 more
doaj   +1 more source

Detection of Tumor Cell-Specific mRNA in the Peripheral Blood of Patients with Breast Cancer-Evaluation of Several Markers with Real-Time Reverse Transcription-PCR [PDF]

, 2013
It is widely known that cells from epithelial tumors, e. g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic ...
Alexandra Kölbl, Alix-Panabieres, Balbin, Bergqvist, Bernard, Bolke, Braun, Braun, Chang, Christian Schindlbeck, De Cremoux, Dontu, Dydensborg, Fabre-Lafay, Fleming, Frasor, Gervasoni, Giani, Guo, Hedenfalk, Inaba, Jiang, Julia Neugebauer, Kim, Klaus Friese, Konecny, Krawczyk, Kurec, Lafleur, Livak, Markou, Matthias Ilmer, Mendoza, Mitas, Moliterni, Nikseresht, Noack, Pan, Pantel, Ring, Selvamurugan, Simone Hofmann, Slamon, Smerage, Solomayer, Stefan Hutter, Tai, Udo Jeschke, Ulrich Andergassen, Vandesompele, Verena Engelstädter, Wang, Weissenstein, Wu, Wu, Zhang   +55 more
core   +3 more sources

Detection of cytomegalovirus using ‘boosted’ nested PCR

Molecular and Cellular Probes, 1995
As a part of a study of an outbreak of CMV infections in a neonatal care intensive care unit, a modified nested PCR was developed for detection of CMV DNA in clinical specimens. Standard nested PCR involves a critical step; passage of PCR products from the first reaction round to the second round.
K L, Borg, S A, Nordbø, P, Winge, A, Dalen   +3 more
openaire   +2 more sources

COMPARATIVE STUDY ON USE OF NESTED PCR AND CONVENTIONAL METHODS IN DIAGNOSIS OF TREATED AND UNTREATED TUBERCULOSIS [PDF]

Acta Medica Iranica, 1997
A nested polymerase chain reaction (PCR) assay with two pairs of primers was applied for the detection of M. tuberculosis complex DNA in specimens of 51 patients.
A.R. Bahrmand1, A.A. Velayati, M. Bahadori, M. Masjedi   +3 more
doaj   +2 more sources

A comparative study on detection of bartonella henselae infection by culture followed by pcr, nested-pcr and ifa [PDF]

Kafkas Universitesi Veteriner Fakültesi Dergisi, 2017
Cats are the main reservoirs of zoonotic Bartonella henselae which are the causative agents of Cat Scratch Disease (CSD). The aim of this study is to compare three diagnostic methods including culture followed by PCR from whole blood, nested-PCR from ...
DIREN SIGIRCI B, CELIK B, BASARAN KAHRAMAN B, GUMUS B, METINER K, ADIGUZEL MC, IKIZ S, BAGCIGIL AF, OZGUR NY, AK S   +9 more
doaj   +1 more source