Results 31 to 40 of about 142,834 (335)

Detection of cytomegalovirus using ‘boosted’ nested PCR

Molecular and Cellular Probes, 1995
As a part of a study of an outbreak of CMV infections in a neonatal care intensive care unit, a modified nested PCR was developed for detection of CMV DNA in clinical specimens. Standard nested PCR involves a critical step; passage of PCR products from the first reaction round to the second round.
K L, Borg, S A, Nordbø, P, Winge, A, Dalen   +3 more
openaire   +2 more sources

COMPARATIVE STUDY ON USE OF NESTED PCR AND CONVENTIONAL METHODS IN DIAGNOSIS OF TREATED AND UNTREATED TUBERCULOSIS [PDF]

Acta Medica Iranica, 1997
A nested polymerase chain reaction (PCR) assay with two pairs of primers was applied for the detection of M. tuberculosis complex DNA in specimens of 51 patients.
A.R. Bahrmand1, A.A. Velayati, M. Bahadori, M. Masjedi   +3 more
doaj   +2 more sources

Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]

Veterinary World, 2017
Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal, Sunit Kumar Mukhopadhayay, Saktipada Pradhan, Samiran Mondal, Chandrakanta Jana, Nimai Chandra Patra, Rabindra Nath Hansda   +6 more
doaj   +1 more source

Efficiency of Rz-1 based rhizomania resistance and molecular studies on BNYVV isolates from sugar beet cultivation in Geece [PDF]

, 2011
A survey was carried out to investigate the current situation concerning rhizomania disease incidence in sugar beet cultivation of Greece. A systematic field evaluation over locations and years revealed a consistent disease severity pattern according to ...
Goldbach, R.W., Pavli, R., Prins, M.W., Skaracis, G.N.   +3 more
core   +2 more sources

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, Thaís da Rocha Boeira, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge   +4 more
doaj   +1 more source

Molecular Genotyping of HBV by using Nested PCR-RFLP among Hepatitis B Patients in Daerah Istimewa Yogyakarta Province and Surrounding Area

Indonesian Journal of Biotechnology, 2008
Hepatitis B virus (HBV) can be classified into 8 genotypes, genotype A to H. Genotype of HBV is important for clinical and etiological investigations. Research for HBV genotyping, HBV transmission study using nested PCR and HBV genotyping based on RFLP ...
Aris Haryanto, Nenny Sri Mulyani, Titis Widowati, Nastiti Wijayanti, Purnomo Hadi   +4 more
doaj   +1 more source

An unusual presentation of a case of human psittacosis [PDF]

, 2018
Background: Chlamydia psittaci is a gram-negative, obligate intracellular organism. Birds are the main reservoir, but also non-avian domestic animals and humans can be infected.
Boelens, Jerina, De Spiegeleer, Anton, Thijs, Elke, Van Braeckel, Eva, Vande Weygaerde, Yannick, Vanrompay, Daisy, Vermaelen, Karim, Versteele, Charlot   +7 more
core   +3 more sources

DNA extraction and Nested PCR v1 [PDF]

, 2019
This is the protocol for the Nested PCR assay
Xiaohua Liu, Jianping Cao, Meng Xu, Ning Xu, Fengkun Yang, Fangwei Wu, Benfu Li, Aiqin Liu *, Yujuan Shen *   +8 more
openaire   +1 more source

Diagnosis of canine coronavirus infection using nested-PCR

Journal of Virological Methods, 2000
The results of polymerase chain reaction (PCR) and nested polymerase chain reaction (n-PCR) assays for the diagnosis of canine coronavirus (CCV) infection, and the comparison with other diagnostic techniques, such as electron microscopy (EM) and virus isolation using A-72 cell line are reported.
Pratelli, Annamaria, Buonavoglia, Domenico, Martella, Vito, Tempesta, Maria, Lavazza, Antonio, Buonavoglia, Canio   +5 more
openaire   +2 more sources

Improved Detection Sensitivity of Spring Viremia of Carp Virus by Substituting a Two-Step with a One-Step Nested Reverse Transcription Polymerase Chain Reaction Method

Microorganisms
Spring viremia of carp (SVC) is a highly contagious disease that affects cyprinids, resulting in systemic hemorrhage, abdominal distension, exophthalmia, and high mortality in juveniles.
Ji-Yoon Park, In-Joo Shin, Hyunwoo Kim, Eun Sup Lee, Euna Choi, Hyoung Jun Kim, Se Ryun Kwon   +6 more
doaj   +1 more source