Results 31 to 40 of about 137,602 (289)

Prevalência da infecção por Ehrlichia em cães e carrapatos no Nordeste do Brasil [PDF]

, 2010
This study investigated the epidemiology of canine ehrlichiosis in Northeastern Brazil, focusing the identification of the Ehrlichia species and vectors involved.
ALCÂNTARA, Adriano Costa De, BARBOZA, Débora Cristina Portela Medina, FERREIRA, Fernando, FRANKE, Carlos Roberto, GONDIM, Luís Fernando Pita, LABRUNA, Marcelo Bahia, LEAL, Danielle Custódio, SOUZA, Bárbara Maria Paraná da Silva, UZÊDA, Rosângela Soares   +8 more
core   +2 more sources

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, Thaís da Rocha Boeira, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge   +4 more
doaj   +1 more source

Single genome sequencing of near full-length HIV-1 RNA using a limiting dilution approach [PDF]

, 2019
Sequencing very long stretches of the HIV-1 genome can advance studies on virus evolution and in vivo recombination but remains technically challenging.
Demecheleer, Els, Hardy, Jarryt, Hebberecht, Laura, Mortier, Virginie, Schauvliege, Marlies, Staelens, Delfien, Vancoillie, Leen, Verhofstede, Chris   +7 more
core   +1 more source

DNA extraction and Nested PCR v1 [PDF]

, 2019
This is the protocol for the Nested PCR assay
Xiaohua Liu, Jianping Cao, Meng Xu, Ning Xu, Fengkun Yang, Fangwei Wu, Benfu Li, Aiqin Liu *, Yujuan Shen *   +8 more
openaire   +1 more source

Molecular Genotyping of HBV by using Nested PCR-RFLP among Hepatitis B Patients in Daerah Istimewa Yogyakarta Province and Surrounding Area

Indonesian Journal of Biotechnology, 2008
Hepatitis B virus (HBV) can be classified into 8 genotypes, genotype A to H. Genotype of HBV is important for clinical and etiological investigations. Research for HBV genotyping, HBV transmission study using nested PCR and HBV genotyping based on RFLP ...
Aris Haryanto, Nenny Sri Mulyani, Titis Widowati, Nastiti Wijayanti, Purnomo Hadi   +4 more
doaj   +1 more source

An unusual presentation of a case of human psittacosis [PDF]

, 2018
Background: Chlamydia psittaci is a gram-negative, obligate intracellular organism. Birds are the main reservoir, but also non-avian domestic animals and humans can be infected.
Boelens, Jerina, De Spiegeleer, Anton, Thijs, Elke, Van Braeckel, Eva, Vande Weygaerde, Yannick, Vanrompay, Daisy, Vermaelen, Karim, Versteele, Charlot   +7 more
core   +3 more sources

Diagnosis of canine coronavirus infection using nested-PCR

Journal of Virological Methods, 2000
The results of polymerase chain reaction (PCR) and nested polymerase chain reaction (n-PCR) assays for the diagnosis of canine coronavirus (CCV) infection, and the comparison with other diagnostic techniques, such as electron microscopy (EM) and virus isolation using A-72 cell line are reported.
Pratelli, Annamaria, Buonavoglia, Domenico, Martella, Vito, Tempesta, Maria, Lavazza, Antonio, Buonavoglia, Canio   +5 more
openaire   +2 more sources

Improved Detection Sensitivity of Spring Viremia of Carp Virus by Substituting a Two-Step with a One-Step Nested Reverse Transcription Polymerase Chain Reaction Method

Microorganisms
Spring viremia of carp (SVC) is a highly contagious disease that affects cyprinids, resulting in systemic hemorrhage, abdominal distension, exophthalmia, and high mortality in juveniles.
Ji-Yoon Park, In-Joo Shin, Hyunwoo Kim, Eun Sup Lee, Euna Choi, Hyoung Jun Kim, Se Ryun Kwon   +6 more
doaj   +1 more source

Molecular evidence for zoonotic transmission of Giardia duodenalis among dairy farm workers in West Bengal, India [PDF]

, 2011
No study in the past has examined the genetic diversity and zoonotic potential of Giardia duodenalis in dairy cattle in India. To assess the importance of these animals as a source of human G.
Amiya Kumar Pramanik, Buret, Caccio, Chanchal Debnath, Deshpande, Ey, Hall, Higgins, Huetink, Islam, Itagaki, Lalle, Langkjaer, Lasek-Nesselquist, Lihua Xiao, Mendonca, Monis, Olson, Olson, O’Handley, O’Handley, Ruest, Sandipan Ganguly, Shahbaz Manzoor Khan, Thompson, Tomoyoshi Nozaki, Trout, Trout, Winkworth, Xiao, Xiao   +30 more
core   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source