Results 61 to 70 of about 142,834 (335)

Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams Nested PCR espécie-específica para o diagnóstico da infecção por Brucella ovis em carneiros

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa, C.N. Nozaki, N.S.C. Lira, J.M.A.P. Antunes, M.N. Xavier, E.A. Costa, T.A. Paixão, R.L. Santos, J. Megid   +8 more
doaj  

Use of a nested PCR-enzyme immunoassay with an internal control to detect Chlamydophila psittaci in turkeys

BMC Infectious Diseases, 2005
Background Laboratory diagnosis of Chlamydophila psittaci, an important turkey respiratory pathogen, is difficult. To facilitate the diagnosis, a nested PCR-enzyme immunoassay (PCR-EIA) was developed to detect the Cp.
Goddeeris Bruno M, Volckaert Guido, Messmer Trudy O, Verminnen Kristel, Van Loock Marnix, Vanrompay Daisy   +5 more
doaj   +1 more source

Detection of Mycobacterium bovis in bovine and bubaline tissues using nested-PCR for TbD1. [PDF]

PLoS ONE, 2014
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates.
Cristina P Araújo, Ana Luiza A R Osório, Kláudia S G Jorge, Carlos Alberto N Ramos, Antonio Francisco S Filho, Carlos Eugênio S Vidal, Eliana Roxo, Christiane Nishibe, Nalvo F Almeida, Antônio A F Júnior, Marcio R Silva, José Diomedes B Neto, Valíria D Cerqueira, Martín J Zumárraga, Flábio R Araújo   +14 more
doaj   +1 more source

Toxoplasma Gondii Identification in Mother\u27s Blood and Fetal Tissue with Nested PCR [PDF]

, 2006
Toxoplasma gondii identification in mother’s blood and fetal tissue with nested PCRObjective: To examine the correlation between Toxoplasma gondii infection with spontaneous abortion on pregnant women based on nested PCR result from mother’s ...
Imada, M. (Mihoko), Masengi-Rumopa, J. A. (Juliana), Rahardjo, T. M. (Theresia), Suryawan, A. (Aloysius), Tuda, J. S. (Josef)   +4 more
core  

Rapid nested-PCR for tyrosinase gene detection on chip [PDF]

Biosensors and Bioelectronics, 2011
The availability of non-invasive, fast and sensitive technologies for detection of circulating cancer cells is still a critical need of clinical oncology, particularly for diagnosis of aggressive and highly metastatic tumors, like malignant melanoma.
AG Sciancalepore, A Polini, E Mele, S Girardo, R Cingolani, D Pisignano   +5 more
openaire   +6 more sources

Self‐Assembling Peptide Hydrogels Support Stromal Vascular Fraction Viability to Promote In Vivo Nerve Regeneration

Advanced Healthcare Materials, EarlyView.
Stromal vascular fraction (SVF) may enhance nerve repair, especially when delivered in a self‐assembling peptide hydrogel (SAPH). In vitro, softer SAPH increased neuronal explant outgrowth and supported greater SVF viability and proliferation. In a rat sciatic defect, SVF in an optimized SAPH produced motor and sensory recovery equivalent to autograft ...
Liam A. McMorrow, Steffan Llewellyn, Jared McSweeney, Alessandro Faroni, Aline F. Miller, Alberto Saiani, Adam J. Reid   +6 more
wiley   +1 more source

Limited asymptomatic carriage of Pneumocystis jiroveci in human immunodeficiency virus-infected patients [PDF]

, 2003
Forty-seven bronchoalveolar lavage fluid samples from 16 human immunodeficiency virus (HIV)-infected patients were used to test the latency model of Pneumocystis infection in the human host.
Ambrose, HE, Denis, CM, Lindley, AR, Miller, RF, Wakefield, AE   +4 more
core  

Modulation of the host Th1 immune response in pigeon protozoal encephalitis caused by Sarcocystis calchasi [PDF]

, 2013
Pigeon protozoal encephalitis (PPE) is an emerging central-nervous disease of domestic pigeons (Columba livia f. domestica) reported in Germany and the United States.
Gruber, Achim D., Kaspers, Bernd, Klopfleisch, Robert, Lierz, Michael, Meyer, Anne, Olias, Philipp   +5 more
core   +3 more sources

High‐Throughput In Vivo Screening Using Barcoded mRNA Identifies Lipid Nanoparticles With Extrahepatic Tropism for In Situ Immunoengineering

Advanced Materials, EarlyView.
A high‐throughput in vivo mRNA LNP screening platform is developed and employed to screen a large library of 122 mRNA LNPs in vivo for delivery to immune, stromal, and parenchymal cells, identifying promising LNP candidates. A novel small particle flow cytometry‐based protein adsorption analysis method is utilized to interrogate protein corona ...
Alex G. Hamilton, Ajay S. Thatte, Junchao Xu, Zhangyi Luo, Hannah C. Safford, Kelsey L. Swingle, Jenna Muscat‐Rivera, Michael Kegel, Xuexiang Han, Ryann A. Joseph, Amanda M. Murray, Hannah C. Geisler, Ricardo C. Whitaker, Lulu Xue, Roman Spektor, Jilian R. Melamed, Drew Weissman, Michael J. Mitchell   +17 more
wiley   +1 more source

Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients Detecção de herpesvirus humano-7 por nested-PCR qualitativo: comparação entre indivíduos sadios e receptores de transplante hepático

Revista da Sociedade Brasileira de Medicina Tropical, 2008
Diagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host.
Ronaldo Luis Thomasini, Juliana de Moraes Martins, Daniela Corte Parola, Sandra Helena Alves Bonon, Ilka de Fátima Santana Ferreira Boin, Luis Sérgio Leonardi, Marília Leonardi, Sandra Cecília Botelho Costa   +7 more
doaj   +1 more source