Results 11 to 20 of about 186,282 (233)
An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection
Revista da Sociedade Brasileira de Medicina Tropical, 2013 Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis. Methods We developed the OTsn-PCR assay for the detection of P. brasiliensis in clinical specimens and
Amanda de Faveri Pitz +6 more
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Frontiers in Veterinary Science, 2020 Porcine cytomegalovirus (PCMV) is a pathogen that must be removed from pigs for use as organ donors in xenotransplantation. Recently, it has been found that when donor pigs are infected with PCMV, a pig-to-non-human-primate xenotransplantation lower ...
Hye-young Wang +4 more
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Nested PCR Analysis of Plasmodium Parasites [PDF]
, 2003 In the natural and untreated intermediate hosts, Plasmodium infections are maintained for extended and often lifelong periods. Parasitemia generally reaches its highest level in the first peak after inoculation, and only rises thereafter during increasingly brief and interspersed episodes. As the host acquires tolerance to higher numbers of multiplying
Snounou, Georges, Balbir, Singh
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Nested PCR for Diagnosis of Typhoid Fever [PDF]
Journal of Clinical Microbiology, 2005 The superiority of the nested PCR employing H 1-d primers over culture or single-dilution Widal (4) should be reevaluated for isolates from unusual sites and from among those coinfected with human immunodeficiency virus (HIV)/AIDS. Numerous extraintestinal complications are known with Salmonella enterica serovar Typhi infection.
Subhash C, Arya +4 more
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Molecular confirmation of Sarcocystis fayeri in a donkey [PDF]
, 2017 Sarcocystis fayeri is a canine protozoan parasite with an equine intermediate host. Historically classified as an incidental pathogen, recent literature has described the toxic effects of Sarcocystis fayeri in human food poisoning, and highlighted ...
Coultous, Robert M. +4 more
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Presence of multiple bacterial markers in clinical samples might be useful for presumptive diagnosis of infection in cirrhotic patients with culture-negative reports [PDF]
, 2016 Bacterial infections in cirrhotic patients with ascites are associated with a severe prognosis and an increased risk of death. The microbiological standard tests for the diagnosis of suspected infection, based on culture test of blood and ascitic fluid ...
Boaretti, M +4 more
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An unusual presentation of a case of human psittacosis [PDF]
, 2018 Background: Chlamydia psittaci is a gram-negative, obligate intracellular organism. Birds are the main reservoir, but also non-avian domestic animals and humans can be infected.
Boelens, Jerina +7 more
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Revista da Sociedade Brasileira de Medicina Tropical, 2010 INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction,
Andrea Cristine Koishi +5 more
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Comparison between four laboratory tests for routine diagnosis of enzootic bovine leukosis [PDF]
Kafkas Universitesi Veteriner Fakültesi Dergisi, 2022 The aim of this study was to compare the diagnostic capabilities of the agar gel immunodiff usion test (AGID) and two types of PCR, nested PCR (with nucleic acid extraction from blood) and direct blood nested PCR (db nested PCR) - without extraction, vs.
Nikolina RUSENOVA +2 more
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Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2013 The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams.
L.F. Costa +8 more
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