Results 21 to 30 of about 186,282 (233)

Nested PCR Assay for Detection of Granulocytic Ehrlichiae [PDF]

Journal of Clinical Microbiology, 1998
ABSTRACT A sensitive and specific nested PCR assay was developed for the detection of granulocytic ehrlichiae. The assay amplifies the 16S rRNA gene and was used to examine acute-phase EDTA-blood and serum samples obtained from seven humans with clinical presentations compatible with human granulocytic ehrlichiosis.
Massung, Robert F., Slater, Kim, Owens, Jessica H., Nicholson, William L., Mather, Thomas N., Solberg, Victoria B., Olson, James G.   +6 more
openaire   +2 more sources

Tracking of Coetaneous Leishmaniasis by Parasitological, Molecular and Biochemical Analysis

Kufa Journal for Nursing Sciences, 2015
Background: In areas of endemicity without sufficient laboratory infrastructure, identification of Leishmaniaparasites is useful for control and preventive plan and very important since species differentiation is instrumental in selection of optimal ...
Sundus Nsaif AL-Hucheimi, Baqur A. Sultan, Muhsin A. Al-Dhalimi, Thikra A. Mahmood   +3 more
doaj   +1 more source

Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patients

BMC Infectious Diseases, 2004
Background Molecular detection of herpesviruses DNA is considered as the reference standard assay for diagnosis of central nervous system infections. In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV ...
Bartolone Rosaria, Giuliani Giuseppe, De Vecchi Elena, Lombardi Alessandra, Drago Lorenzo, Gismondo Maria   +5 more
doaj   +1 more source

A comparative study on detection of bartonella henselae infection by culture followed by pcr, nested-pcr and ifa [PDF]

Kafkas Universitesi Veteriner Fakültesi Dergisi, 2017
Cats are the main reservoirs of zoonotic Bartonella henselae which are the causative agents of Cat Scratch Disease (CSD). The aim of this study is to compare three diagnostic methods including culture followed by PCR from whole blood, nested-PCR from ...
DIREN SIGIRCI B, CELIK B, BASARAN KAHRAMAN B, GUMUS B, METINER K, ADIGUZEL MC, IKIZ S, BAGCIGIL AF, OZGUR NY, AK S   +9 more
doaj   +1 more source

Rapid microarray-based method for monitoring of all currently known single-nucleotide polymorphisms associated with parasite resistance to antimalaria drugs. [PDF]

, 2007
Parasite drug resistance is partly conferred by single-nucleotide polymorphisms (SNPs), and monitoring them has been proposed as an alternative to monitoring drug resistance.
Crameri, Andreas, Marfurt, Jutta, Mugittu, Kefas, Maire, Nicolas, Regös, Attila, Coppee, Jean Yves, Sismeiro, Odile, Burki, Richard, Huber, Eric, Laubscher, Daniel, Puijalon, Odile, Genton, Blaise, Felger, Ingrid, Beck, Hans-Peter   +13 more
core   +1 more source

Symbiont diversity is not involved in depth acclimation in the Mediterranean sea whip Eunicella singularis [PDF]

, 2011
In symbiotic cnidarians, acclimation to depth and lower irradiance can involve physiological changes in the photosynthetic dinoflagellate endosymbiont, such as increased chlorophyll content, or qualitative modifications in the symbiont population in ...
Allemand, D., Caligara, C., Cerrano, C., Ciosi, M., Forcioli, D., Francour, P., Merle, P.L., Muti, C.   +7 more
core   +2 more sources

COMPARATIVE STUDY ON USE OF NESTED PCR AND CONVENTIONAL METHODS IN DIAGNOSIS OF TREATED AND UNTREATED TUBERCULOSIS [PDF]

Acta Medica Iranica, 1997
A nested polymerase chain reaction (PCR) assay with two pairs of primers was applied for the detection of M. tuberculosis complex DNA in specimens of 51 patients.
A.R. Bahrmand1, A.A. Velayati, M. Bahadori, M. Masjedi   +3 more
doaj   +2 more sources

Development of nested polymerase chain reaction-based diagnosis of duck enteritis virus and detection of DNA polymerase gene from non-descriptive duck breeds of West Bengal, India [PDF]

Veterinary World, 2017
Aim: The study was undertaken to detect the clinical signs, postmortem lesions of embryonated duck plague (DP) infected eggs, and histopathological changes of chorioallantoic membrane (CAM) in non-descriptive ducks of West Bengal with special reference ...
Partha Sarathi Mandal, Sunit Kumar Mukhopadhayay, Saktipada Pradhan, Samiran Mondal, Chandrakanta Jana, Nimai Chandra Patra, Rabindra Nath Hansda   +6 more
doaj   +1 more source

Avian malaria is absent in juvenile colonial herons (Ardeidae) but not Culex pipiens mosquitoes in the Camargue, Southern France [PDF]

, 2015
Apicomplexan blood parasites Plasmodium and Haemoproteus (together termed “Avian malaria”) and Leucocytozoon are widespread, diverse vector-transmitted blood parasites of birds, and conditions associated with colonial nesting in herons (Ardeidae) and ...
Gauthier-Clerc, Michel, Larcombe, Stephen D.   +1 more
core   +1 more source

Human papillomavirus detection and typing using a nested-PCR-RFLP assay

Brazilian Journal of Infectious Diseases
BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to ...
Janaina Coser, Thaís da Rocha Boeira, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge   +4 more
doaj   +1 more source