Results 41 to 50 of about 186,282 (233)
Detection of cytomegalovirus using ‘boosted’ nested PCR
Molecular and Cellular Probes, 1995 As a part of a study of an outbreak of CMV infections in a neonatal care intensive care unit, a modified nested PCR was developed for detection of CMV DNA in clinical specimens. Standard nested PCR involves a critical step; passage of PCR products from the first reaction round to the second round.
K L, Borg +3 more
openaire +2 more sources
Epidemiology and fitness effects of wood mouse herpesvirus in a natural host population [PDF]
, 2012 Rodent gammaherpesviruses have become important models for understanding human herpesvirus diseases. In particular, interactions between murid herpesvirus 4 and Mus musculus (a non-natural host species) have been extensively studied under controlled ...
Amy B. Pedersen +12 more
core +1 more source
FEBS Open Bio, EarlyView.ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand +2 more
wiley +1 more source
Toxoplasma Gondii Identification in Mother\u27s Blood and Fetal Tissue with Nested PCR [PDF]
, 2006 Toxoplasma gondii identification in mother’s blood and fetal tissue with nested PCRObjective: To examine the correlation between Toxoplasma gondii infection with spontaneous abortion on pregnant women based on nested PCR result from mother’s ...
Imada, M. (Mihoko) +4 more
core
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
PCR-based assays for detecting Xanthomonas axonopodis pv. allii in onion seed [PDF]
, 2012 Bacterial blight of onion is an emerging disease threatening world onion production, and causing damage to other Allium crops. The causal agent, Xanthomonas axonopodis pv.
Chabirand, Aude +4 more
core
Expression of plasma prekallikrein mRNA in human nonhepatic tissues and cell lineages suggests special local functions of the enzyme [PDF]
, 1999 At present it is generally accepted that plasma prekallikrein (PPK) is synthesized in the liver and secreted into the bloodstream. Surprisingly, it has recently been shown that PPK mRNA is present also in RNA from the kidney, adrenal gland and placenta ...
Arnhold, Marianne +4 more
core +1 more source
Advanced Healthcare Materials, EarlyView.We developed a micro‐sized, biocompatible implant for postoperative sustained delivery of anti‐fibrotic antibodies in glaucoma surgery. Machine learning‐guided optimization of polymer composition, implant geometry, and porosity enabled precise control of drug release.
Mengqi Qin +5 more
wiley +1 more source
Revista Brasileira de Parasitologia Veterinária, 2018 This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-
Vinícius da Silva Camargo +6 more
doaj +1 more source
A Real-Time PCR Assay for Detection of Low Pneumocystis jirovecii Levels
Frontiers in Microbiology, 2022 Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii.
Susana Ruiz-Ruiz +18 more
doaj +1 more source