Results 51 to 60 of about 186,282 (233)
Improved quantitative PCR using nested primers. [PDF]
Genome Research, 1994 Quantitative PCR can often be improved by conducting the amplification with nested primers. First, fewer nonspecific amplification products, which could otherwise interfere with quantitation, are produced. Often, nonspecific products can be eliminated. In these cases, relatively simple nonspecific detection techniques are suitable for quantitation.
openaire +2 more sources
Application of a Nested, Multiplex PCR to Psittacosis Outbreaks [PDF]
Journal of Clinical Microbiology, 1997 We developed a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian specimens. The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present in clinical specimens. The target sequence was the 16S rRNA gene.
T O, Messmer +4 more
openaire +3 more sources
Molecular Detection of Anaplasma bovis in Cattle from Central Part of Iran [PDF]
, 2010 Anaplasma bovis is a leukocytotropic agent of bovine anaplasmosis and there is no available information about molecular study on this agent in cattle of Iran. In this study a total 150 cattle blood samples were collected from central part of Iran.
Parviz Shayan, Vahid Noaman
core
Molecular surveillance of Theileria ovis, Theileria lestoquardi and Theileria annulata infection in sheep and ixodid ticks in Iran [PDF]
, 2013 A molecular study was undertaken to detect Theileria ovis, Theileria lestoquardi and Theileria annulatain sheep and tick vectors. Investigation was conducted from 2010 to 2011 in the south of Khorasan Razavi Province, Iran.
Gholamreza Razmi, Saeed Yaghfoori
core +1 more source
Advanced Science, EarlyView.This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang +13 more
wiley +1 more source
Research in Molecular Medicine, 2019 Background: Bovine leukaemia virus (BLV) is the cause of enzootic bovine leukaemia which belongs to retroviruses including Human T-cell leukaemia virus and simian T-lymphotropic virus. Due to this familiarity, the possibility of virus (BLV) transfer from
Camellia Taghadosi +4 more
doaj
Pesquisa Veterinária Brasileira, 2009 Pneumocystis has been isolated from a wide range of unrelated mammalian hosts, including humans, domestic and wild animals. It has been demonstrated that the genome of Pneumocystis of one host differs markedly from that of other hosts. Also, variation in
Edna Maria Cavallini Sanches +9 more
doaj +1 more source
DNA extraction and Nested PCR v1 [PDF]
, 2019 This is the protocol for the Nested PCR assay
Xiaohua Liu +8 more
openaire +1 more source
Cryptosporidium infection in patients with gastroenteritis in Sari, Iran [PDF]
, 2014 Background: Cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries.
Ahmadpour, E. +6 more
core
Astrocyte Enrichment of 3D Cortical Constructs Enhances Brain Repair
Advanced Science, EarlyView.This study highlights the role of astrocytes in supporting neural progenitor cell survival and differentiation after traumatic brain injury. Astrocytes enhanced neuronal differentiation, improved cell survival in co‐cultures, and promoted integration of microfluidics‐based implants with host tissue following implantation. Additionally, increased axonal
Elisa M. Cruz +20 more
wiley +1 more source