Results 1 to 10 of about 9,796 (161)

A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model [PDF]

BMC Genomics, 2017
Background In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D ...
Felix Schulze, Deeksha Malhan, Thaqif El Khassawna, Christian Heiss, Anja Seckinger, Dirk Hose, Angela Rösen-Wolff   +6 more
doaj   +4 more sources

ACTB, CDKN1B, GAPDH, GRB2, RHOA and SDCBP Were Identified as Reference Genes in Neuroendocrine Lung Cancer via the nCounter Technology. [PDF]

PLoS ONE, 2016
Neuroendocrine lung cancer (NELC) represents 25% of all lung cancer cases and large patient collectives exist as formalin-fixed, paraffin-embedded (FFPE) tissue only.
Robert Fred Henry Walter, Robert Werner, Claudia Vollbrecht, Thomas Hager, Elena Flom, Daniel Christian Christoph, Jan Schmeller, Kurt Werner Schmid, Jeremias Wohlschlaeger, Fabian Dominik Mairinger   +9 more
doaj   +4 more sources

Tramesan, a novel polysaccharide from trametes versicolor. structural characterization and biological effects [PDF]

, 2017
Mushrooms represent a formidable source of bioactive compounds. Some of these may be considered as biological response modifiers; these include compounds with a specific biological function: antibiotics (e.g.
Scarpari, Marzia, Reverberi, Massimo, Parroni, Alessia, Scala, Valeria, Fanelli, Corrado, Pietricola, Chiara, Zjalic, Slaven, Maresca, Vittoria, Tafuri, Agostino, Ricciardi, Maria R., Licchetta, Roberto, Mirabilii, Simone, Sveronis, Aris, Cescutti, Paola, Rizzo, Roberto   +14 more
core   +24 more sources

Identification and validation of reference genes for qPCR of Pseudomonas aeruginosa L10 under varying n-hexadecane concentrations [PDF]

BMC Microbiology
Pseudomonas aeruginosa can efficiently degrades petroleum hydrocarbons, but its degradation rate is affected by hydrocarbon pollutant concentrations, which must be neither too high nor too low. To quantitatively analyze P.
Huan Wang, Xiaobing Wang, Juntao Chen, Yumiao Zhang, Junhua Liu, Fangliang Xia, Jian Li, Tao Wu, Longxiang Liu   +8 more
doaj   +2 more sources

Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.). [PDF]

PLoS ONE, 2015
Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts.
Roberta Fogliatto Mariot, Luisa Abruzzi de Oliveira, Marleen M Voorhuijzen, Martijn Staats, Ronald C B Hutten, Jeroen P Van Dijk, Esther Kok, Jeverson Frazzon   +7 more
doaj   +1 more source

Reference gene selections for real time quantitative PCR analysis of gene expression in different oat tissues and under salt stress

Biologia Plantarum, 2020
Appropriate choice of reference genes for data normalization is of critical importance for accurate real time reverse transcription quantitative PCR (RT-qPCR) analysis of gene expression.
Z.L. DUAN, W.H. HAN, L. YAN, B. WU
doaj   +1 more source

Transcriptome-based validation of proper reference genes for reverse trascription quantitative PCR analysis of Sinocalycanthus chinensis

Biologia Plantarum, 2020
Reverse transcription quantitative PCR is a widely used method to detect gene expressions. To obtain accurate expression results, the selection of proper reference genes is important and necessary.
C. ZHANG, Q.-N. JIANG, Y.-G. WANG, J.-X. FU, B. DONG, L.-H. ZHOU, H.-B. ZHAO   +6 more
doaj   +1 more source

Transcriptome-based screening and the optimal reference genes for real-time quantitative PCR in Rehmannia chingii and R. henryi

Biologia Plantarum, 2020
Real time quantitative PCR (qPCR) is a powerful tool for studying the expression of specific genes. The accuracy and reliability of qPCR analysis data require the selection of reference genes with stable expression.
X. ZUO, F.-Q. WANG, X.-R. LI, M.-M. LI
doaj   +1 more source

Stability analysis of reference genes for RT-qPCR assays involving compatible and incompatible Ralstonia solanacearum-tomato ‘Hawaii 7996’ interactions

Scientific Reports, 2021
Reverse transcription-quantitative PCR (RT-qPCR) is an analytical tool for gene expression quantification. Reference genes are not yet available for gene expression analysis during interactions of Ralstonia solanacearum with ‘Hawaii 7996’ (the most ...
Greecy M. R. Albuquerque, Fernando C. A. Fonseca, Leonardo S. Boiteux, Rafaela C. F. Borges, Robert N. G. Miller, Carlos A. Lopes, Elineide B. Souza, Maria Esther N. Fonseca   +7 more
doaj   +1 more source

Selection and validation of reference genes by RT-qPCR under photoperiodic induction of flowering in sugarcane (Saccharum spp.)

Scientific Reports, 2021
Although reference genes have previously been used in the expression analysis of genes involved in sugarcane flowering they had not been experimentally validated for stability and consistency of expression between different samples over a wide range of ...
Paulo H. da Silva Santos, João R. Vieira Manechini, Michael S. Brito, Elisson Romanel, Renato Vicentini, Maximiliano Scarpari, Stephen Jackson, Luciana R. Pinto   +7 more
doaj   +1 more source