Results 11 to 20 of about 9,796 (161)

Selection of reference genes for normalization of real-time PCR data in visceral adipose tissue of female rats on a fructose-enriched diet [PDF]

Archives of Biological Sciences, 2012
Considering the association of increased fructose intake with the rise in metabolic syndrome and gender differences in its incidence, this study was aimed at evaluating and identifying the optimal reference genes for the relative quantification of ...
Elaković Ivana, Nestorov Jelena, Kovačević Sanja, Matić Gordana   +3 more
doaj   +1 more source

Reference gene selection and RNA preservation protocol in the cat flea, Ctenocephalides felis, for gene expression studies [PDF]

, 2016
This work was supported by a Knowledge Transfer Network BBSRC Industrial Case (#414 BB/L502467/1) studentship in association Zoetis Inc.Peer ...
Baird, John, Bowman, Alan S, Campbell, Ewan M, McIntosh, Catriona H, Woods, Debra J., Zinser, Erich   +5 more
core   +1 more source

Validation of suitable internal control genes for expression studies in aging. [PDF]

, 2009
Quantitative data from experiments of gene expression are often normalized through levels of housekeeping genes transcription by assuming that expression of these genes is highly uniform. This practice is being questioned as it becomes increasingly clear
Bacalini, MARIA GIULIA, Burkle, A, Caiafa, Paola, Calabrese, Roberta, Chevanne, M, Ciccarone, Fabio, Guastafierro, Tiziana, MORENO VILLANUEVA, M, Reale, Anna, Zampieri, Michele   +9 more
core   +1 more source

Gene Expression Studies in Formalin-Fixed Paraffin-Embedded Samples of Cutaneous Cancer: The Need for Reference Genes

Current Issues in Molecular Biology, 2021
Formalin-fixed paraffin-embedded (FFPE) tumour samples may provide crucial data regarding biomarkers for neoplasm progression. Analysis of gene expression is frequently used for this purpose.
Omar García-Pérez, Leticia Melgar-Vilaplana, Elizabeth Córdoba-Lanús, Ricardo Fernández-de-Misa   +3 more
doaj   +1 more source

Methylation-dependent PAD2 upregulation in multiple sclerosis peripheral blood [PDF]

, 2012
Background: Peptidylarginine deiminase 2 (PAD2) and peptidylarginine deiminase 4 (PAD4) are two members of PAD family which are over-expressed in the multiple sclerosis (MS) brain.
ANNIBALI, Viviana, CAIAFA, Paola, CALABRESE, ROBERTA, CICCARONE, FABIO, COARELLI, GIULIA, GUASTAFIERRO, Tiziana, MECHELLI, Rosella, SALVETTI, Marco, UMETON, RENATO, ZAMPIERI, Michele   +9 more
core   +1 more source

Reference gene validation for RT-qPCR, a note on different available software packages. [PDF]

PLoS ONE, 2015
An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR). It is widely supported to identify and validate stable reference genes, since no single biological gene is ...
Ward De Spiegelaere, Jutta Dern-Wieloch, Roswitha Weigel, Valérie Schumacher, Hubert Schorle, Daniel Nettersheim, Martin Bergmann, Ralph Brehm, Sabine Kliesch, Linos Vandekerckhove, Cornelia Fink   +10 more
doaj   +1 more source

Identification of stable endogenous reference genes for real-time PCR in the human fetal gonad using an external standard technique [PDF]

, 2011
Peer ...
Ana Monteiro, Andersen, Baker, Bower, Ellefsen, Huggett, Johnson, O'Shaughnessy, O'Shaughnessy, Paul A. Fowler, Peter J. O'Shaughnessy, Rajapaksha, Smith, Vandesompele   +13 more
core   +1 more source

Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous Frankliniella occidentalis. [PDF]

PLoS ONE, 2015
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes.
Chunxiao Yang, Hui Li, Huipeng Pan, Yabin Ma, Deyong Zhang, Yong Liu, Zhanhong Zhang, Changying Zheng, Dong Chu   +8 more
doaj   +1 more source

Selection and Validation of Potential Reference Genes for Quantitative Real-Time PCR Analysis in Blaptica Dubia (Serville, 1838) (Blattidae, Blaberidae)

Cumhuriyet Science Journal, 2022
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an effective, reproducible, and dependable method for evaluating and targeting expression of genes.
Emin Ufuk Karakaş, Şeyda Berk, Ayşe Nur Pektaş   +2 more
doaj   +1 more source

Housekeeping genes for quantitative expression studies in the three-spined stickleback Gasterosteus aculeatus [PDF]

, 2008
Background During the last years the quantification of immune response under immunological challenges, e.g. parasitation, has been a major focus of research.
A Bas, A Radonic, A Szabo, A Zapata, AL Filby, B Liss, C Ramakers, CL Andersen, EO Koppang, F Haller, G Rauch, HC Ingerslev, J Vandesompele, Joern P Scharsack, JP Scharsack, K Pechhold, KM Wegner, MF Oleksiak, MW Pfaffl, N Nicot, PA Olsvik, S Becker, SA Bustin, SA Bustin, Sascha Hibbeler, SF Gonzalez, SM Jorgensen, Sven Becker, TBH Reusch, V Ransbotyn   +29 more
core   +3 more sources