Results 31 to 40 of about 9,796 (161)

Verification of suitable and reliable reference genes for quantitative real-time PCR during adipogenic differentiation in porcine intramuscular stromal-vascular cells

Animal, 2016
Intramuscular fat (IMF) is an important trait influencing meat quality, and intramuscular stromal-vascular cell (MSVC) differentiation is a key factor affecting IMF deposition.
X. Li, K. Huang, F. Chen, W. Li, S. Sun, X.-E. Shi, G. Yang   +6 more
doaj   +1 more source

Housekeeping gene stability in Pesudomonas aeruginosa PAO1 under the pressure of commonly used antibiotics in molecular microbiology assays

Frontiers in Microbiology, 2023
Pseudomonas aeruginosa is an opportunistic human pathogen notorious for its remarkable capacity of multi-drug resistance, and has become one of the most important model bacteria in clinical bacteriology research.
Lingning Meng, Xiaoli Cao, Chuchu Li, Jia Li, Hui Xie, Jiping Shi, Mei Han, Han Shen, Chang Liu   +8 more
doaj   +1 more source

Identification of new reference genes for the normalisation of canine osteoarthritic joint tissue transcripts from microarray data [PDF]

, 2007
Background Real-time reverse transcriptase quantitative polymerase chain reaction (real-time RT-qPCR) is the most accurate measure of gene expression in biological systems. The comparison of different samples requires the transformation of data through a
Clements, Dylan N., Day, Philip, Day, Philip J R, Maccoux, Lindsey J., Marriage, Fiona, Salway, Fiona   +5 more
core   +4 more sources

Selection of reference genes for quantitative gene expression normalization in flax (Linum usitatissimum L.)

BMC Plant Biology, 2010
Background Quantitative real-time PCR (qRT-PCR) is currently the most accurate method for detecting differential gene expression. Such an approach depends on the identification of uniformly expressed 'housekeeping genes' (HKGs).
Neutelings Godfrey, Hawkins Simon, Huis Rudy   +2 more
doaj   +1 more source

Validation of reference genes for real-time PCR of reproductive system in the black tiger shrimp. [PDF]

PLoS ONE, 2012
Gene expression of reproductive system of the black tiger shrimp (Peneaus monodon) has been widely studied to address poor maturation problem in captivity.
Rungnapa Leelatanawit, Amornpan Klanchui, Umaporn Uawisetwathana, Nitsara Karoonuthaisiri   +3 more
doaj   +1 more source

Validation of miRNA genes suitable as Reference genes in qPCR analyses of miRNA gene expression in Atlantic salmon (Salmo salar) [PDF]

, 2014
Background MicroRNAs (miRNAs) are an abundant class of endogenous small RNA molecules that downregulate gene expression at the post-transcriptional level.
Andreassen, Rune, Johansen, Ilona
core   +2 more sources

Bypassing hazard of housekeeping genes: Their evaluation in rat granule neurons treated with cerebrospinal fluid of Multiple Sclerosis subjects

Frontiers in Cellular Neuroscience, 2015
Gene expression studies employing real-time PCR has become an intrinsic part of biomedical research. Appropriate normalization of target gene transcript(s) based on stably expressed housekeeping genes is crucial in individual experimental conditions to ...
Deepali eMathur, Deepali eMathur, Gerardo eLopez-Rodas, Juan eUreña-Peralta, Bonaventura eCasanova, Francisco eCoret-Ferrer, Maria eBurgal-Marti   +6 more
doaj   +1 more source

Endogenous reference RNAs for microRNA quantitation in formalin-fixed, paraffin-embedded lymph node tissue [PDF]

, 2018
Lymph node metastasis is one of the most important factors for tumor dissemination. Quantifying microRNA (miRNA) expression using real-time PCR in formalin-fixed, paraffin-embedded (FFPE) lymph node can provide valuable information regarding the ...
Cho-Isoda Yukiko, Hori Mitsuo, Iijima Tatsuo, Inada Katsushige, Ishiguro Shingo, Kojima Hiroshi, Oki Akinori, Okoshi Yasushi, Saito Hitoaki, Shimazui Toru, Suzuki Hisashi, Tamaki Yoshio, 大越 靖, 小島 寛, 島居 徹, 沖 明典, 玉木 義雄, 鈴木 久史   +17 more
core   +2 more sources

Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells [PDF]

, 2013
Background: Selecting stably expressed reference genes is essential for proper reverse transcription quantitative polymerase chain reaction gene expression analysis. However, this choice is not always straightforward. In the case of differentiating human
De Sutter, Petra, Deforce, Dieter, Heindryckx, Björn, O'Leary, Thomas, Van Neste, Christophe, Vandesompele, Jo, Vossaert, Liesbeth   +6 more
core   +2 more sources

Identification and Evaluation of Reference Genes for Accurate Transcription Normalization in Safflower under Different Experimental Conditions.

PLoS ONE, 2015
Safflower (Carthamus tinctorius L.) has received a significant amount of attention as a medicinal plant and oilseed crop. Gene expression studies provide a theoretical molecular biology foundation for improving new traits and developing new cultivars ...
Dandan Li, Bo Hu, Qing Wang, Hongchang Liu, Feng Pan, Wei Wu   +5 more
doaj   +1 more source