Results 41 to 50 of about 9,815 (163)
Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process [PDF]
, 2008 12 pages, 2 figures, 4 tables.-- PMID: 19102748 [PubMed].-- PMCID: PMC2629474.[Background] The elucidation of gene expression patterns leads to a better understanding of biological processes.
Marino Expósito-Rodríguez +3 more
core +2 more sources
Selection of miRNA reference genes for plant defence studies in rice (Oryza sativa)
, 2019 RT-qPCR is a widely used method to investigate the expression levels of genes under certain conditions. A key step, however, to have reliable results is the normalization of expression.
De Meyer, Tim +3 more
core +1 more source
Validation of miRNA genes suitable as Reference genes in qPCR analyses of miRNA gene expression in Atlantic salmon (Salmo salar) [PDF]
, 2014 Background MicroRNAs (miRNAs) are an abundant class of endogenous small RNA molecules that downregulate gene expression at the post-transcriptional level.
Andreassen, Rune, Johansen, Ilona
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British Journal of Pharmacology, EarlyView.Abstract Background and Purpose Extensive evidence indicates that lipid accumulation causes renal tubular injury, which further contributes to diabetic nephropathy (DN) progression. Short‐chain fatty acids (SCFAs) play an important role in the maintenance of cellular metabolic health.
Jiayin Tao +12 more
wiley +1 more source
BMC Research Notes, 2013 Background The use of quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) has become widely applied as a method to measure transcript abundance. In order to be reflective of biological processes during health and disease this
Wei Ruoxin +2 more
doaj +1 more source
Bio-Protocol, 2023 When performing expression analysis either for coding RNA (e.g., mRNA) or non-coding RNA (e.g., miRNA), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a widely used method.
Patrick Petersen +3 more
doaj +1 more source
The validation of Short Interspersed Nuclear Elements (SINEs) as a RT-qPCR normalization strategy in a rodent model for temporal lobe epilepsy [PDF]
, 2019 Background : In gene expression studies via RT-qPCR many conclusions are inferred by using reference genes. However, it is generally known that also reference genes could be differentially expressed between various tissue types, experimental conditions ...
Crans, René +8 more
core +4 more sources
Food and Energy Security, Volume 15, Issue 2, March/April 2026.ABSTRACT Cold stress represents one of the primary abiotic stresses affecting forage growth, development, yield, and quality. As a novel agricultural nanomaterial, silicon nanoparticles (SiNPs) demonstrate unique potential in regulating plant stress responses. However, their mechanism of action in forage cold responses remains poorly understood.
Yancui Zhao +6 more
wiley +1 more source
Guangdong nongye kexue【Objective】Real-time quantitative PCR is one of the most conventional and efficient techniques for studying the mRNA expression level of target genes. The proper selection of reference genes is of great significance for the accuracy of the results.
Benshui SHU +6 more
doaj +1 more source
Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular Dystrophy [PDF]
Background:Dogs with dystrophin-deficient muscular dystrophy are valuable models of the equivalent human disease, Duchenne Muscular Dystrophy (DMD): unlike the mdx mouse, these animals present a disease severity and progression that closely matches that ...
Aartsma-Rus +50 more
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