Results 41 to 50 of about 9,796 (161)

Evaluation of stable reference genes in white mustard (Sinapis alba) for qRT-PCR analysis under various stress conditions

The Indian Journal of Agricultural Sciences, 2020
White mustard (Sinapis alba L.) is a member of Brassicaceae family and is a source of various biotics stress resistance genes. S. alba is closely related to the cultivated Brassica juncea which exhibit susceptibility towards various pathogens ...
SHIKHA DIXIT, VINOD KUMAR JANGID, ANITA GROVER   +2 more
doaj   +1 more source

The validation of Short Interspersed Nuclear Elements (SINEs) as a RT-qPCR normalization strategy in a rodent model for temporal lobe epilepsy [PDF]

, 2019
Background : In gene expression studies via RT-qPCR many conclusions are inferred by using reference genes. However, it is generally known that also reference genes could be differentially expressed between various tissue types, experimental conditions ...
Crans, René, Daelemans, Sofie, De Deyn, Peter P, Janssens, Jana, Raedt, Robrecht, Stove, Christophe, Van Craenenbroeck, Kathleen, Van Dam, Debby, Wouters, Elise   +8 more
core   +4 more sources

Determination of qPCR Reference Genes Suitable for Normalizing Gene Expression in a Canine Model of Duchenne Muscular Dystrophy [PDF]

Background:Dogs with dystrophin-deficient muscular dystrophy are valuable models of the equivalent human disease, Duchenne Muscular Dystrophy (DMD): unlike the mdx mouse, these animals present a disease severity and progression that closely matches that ...
Aartsma-Rus, Ali, Andersen, Bages, Bello, Bish, Breitbart, Brinkmeyer-Langford, Burton, Bustin, Bustin, Camerino, Cohn, Cotten, Craig, Crist, Duan, Falzarano, Feder, Fjeldbo, Flanigan, Galindo, Gong, Hildyard, Kallestad, Kornegay, Kornegay, Li, Liu, Mah, McGreevy, Nghiem, Pegoraro, Pfaffl, Piva, Reinig, Rho, Robinson-Hamm, Robriquet, Schmittgen, Solano, Spassov, Swaggart, Thellin, Vieira, Vieira Natassia, Walmsley, Webster, Wuebbles, Yu, Zammit   +50 more
core   +1 more source

Sodium butyrate ameliorates renal tubular lipid accumulation through a protein phosphatase 2A/transcription factor EB axis in diabetic nephropathy

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose Extensive evidence indicates that lipid accumulation causes renal tubular injury, which further contributes to diabetic nephropathy (DN) progression. Short‐chain fatty acids (SCFAs) play an important role in the maintenance of cellular metabolic health.
Jiayin Tao, Sinan Ai, Yaoxian Wang, Yuning Liu, Jingwei Zhou, Qingqing Liu, Xu Wang, Danting Li, Xin Li, Lin Wang, Jinyi Hou, Liang Peng, Zhen Wang   +12 more
wiley   +1 more source

Suitability of endogenous reference genes for gene expression studies with human intraocular endothelial cells

BMC Research Notes, 2013
Background The use of quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) has become widely applied as a method to measure transcript abundance. In order to be reflective of biological processes during health and disease this
Wei Ruoxin, Stewart Elizabeth Anne, Amoaku Winfried M   +2 more
doaj   +1 more source

Expression Stability Analysis of Candidate References for Normalization of RT-qPCR Data Using RefSeeker R package

Bio-Protocol, 2023
When performing expression analysis either for coding RNA (e.g., mRNA) or non-coding RNA (e.g., miRNA), reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is a widely used method.
Patrick Petersen, Joanna Lopacinska-Jørgensen, Claus Høgdall, Estrid Høgdall   +3 more
doaj   +1 more source

Identification and evaluation of suitable reference genes for gene expression studies in the whitefly Bemisia tabaci (Asia I) by reverse transcription quantitative realtime PCR [PDF]

, 2014
This study presents a reliable method for performing reverse transcription quantitative realtime PCR (RT-qPCR) to measure gene expression in the whitefly Bemisia tabaci (Asia I) (Gennadius) (Hemiptera: Aleyrodidae), utilising suitable reference genes for
Carl Collins, David Bailey, Dinsdale A, John Colvin, Mitulkumar V. Patel, Scharlaken B, Susan Seal   +6 more
core   +1 more source

Seed shattering in a North American Oryzeae grain: Developmental and genomic signatures of early domestication

The Plant Genome, Volume 19, Issue 1, March 2026.
Abstract Northern Wild Rice (NWR; Zizania palustris L.) is an aquatic grain endemic to North America and a member of the Oryzeae tribe. As an outcrossing crop with a short breeding history, domestication progress in cultivated NWR (cNWR) is ongoing, and seed shattering remains a major barrier to yield stability.
Reneth Millas, Lillian McGilp, Alan Mickelson, Maybell Banting, Claudia Castell‐Miller, Jennifer Kimball   +5 more
wiley   +1 more source

Expression Stability of Reference Genes in Larvae of Spodoptera frugipferda Under Azadirachtin Stress by Real-Time Quantitative PCR Analysis

Guangdong nongye kexue
【Objective】Real-time quantitative PCR is one of the most conventional and efficient techniques for studying the mRNA expression level of target genes. The proper selection of reference genes is of great significance for the accuracy of the results.
Benshui SHU, Yuting HUANG, Xuanyue YU, Cuiting LIU, Xinyi XIE, Hao SHEN, Jintian LIN   +6 more
doaj   +1 more source

Selection of miRNA reference genes for plant defence studies in rice (Oryza sativa)

, 2019
RT-qPCR is a widely used method to investigate the expression levels of genes under certain conditions. A key step, however, to have reliable results is the normalization of expression.
De Meyer, Tim, De Smet, Lien, Kyndt, Tina, Verstraeten, Bruno   +3 more
core   +1 more source