Results 51 to 60 of about 9,815 (163)

Selection of internal reference genes for SYBR green qRT-PCR studies of rhesus monkey () tissues [PDF]

, 2008
Background: The rhesus monkey (Macaca mulatta) is a valuable and widely used model animal for biomedical research. However, quantitative analyses of rhesus gene expression profiles under diverse experimental conditions are limited by a shortage of ...
K Ahn, Jae Won Huh, Sang Je Park, Dae Soo Kim, H S Ha, Y J Kim, J R Lee, Kyu Tae Chang, H S Kim   +8 more
core   +1 more source

Modulatory Effects of Cirsimarin on Lung Cancer A549 Cells Migration in 2D and 3D Cultures Involves Transcriptional Regulation of Metalloproteinases

Journal of Biochemical and Molecular Toxicology, Volume 40, Issue 3, March 2026.
In 2D culture, the cirsimarin (i) decreased cell viability in both assays, (ii) induced apoptosis, (iii) reduced clonogenicity, (iv) prevented cell migration, (v) induced DNA damage, and (vi) negatively modulated gene expression. In the 3D model, reductions in spheroid viability, area, and cell migration were also observed.
Anna Gabriele Prado dos Santos, Celina Yung‐Ai Lin Lee, Érica Romão Pereira, Andresa Hiromi Sakai, Diego Luís Ribeiro, Victor Antônio Silva Lima, Marcos Bispo Pinheiro Camara, Cláudia Quintino da Rocha, Sabine A. S. Langie, Ilce Mara de Syllos Cólus, Juliana Mara Serpeloni   +10 more
wiley   +1 more source

Endogenous reference RNAs for microRNA quantitation in formalin-fixed, paraffin-embedded lymph node tissue [PDF]

, 2018
Lymph node metastasis is one of the most important factors for tumor dissemination. Quantifying microRNA (miRNA) expression using real-time PCR in formalin-fixed, paraffin-embedded (FFPE) lymph node can provide valuable information regarding the ...
Cho-Isoda Yukiko, Hori Mitsuo, Iijima Tatsuo, Inada Katsushige, Ishiguro Shingo, Kojima Hiroshi, Oki Akinori, Okoshi Yasushi, Saito Hitoaki, Shimazui Toru, Suzuki Hisashi, Tamaki Yoshio, 大越 靖, 小島 寛, 島居 徹, 沖 明典, 玉木 義雄, 鈴木 久史   +17 more
core   +2 more sources

Seed shattering in a North American Oryzeae grain: Developmental and genomic signatures of early domestication

The Plant Genome, Volume 19, Issue 1, March 2026.
Abstract Northern Wild Rice (NWR; Zizania palustris L.) is an aquatic grain endemic to North America and a member of the Oryzeae tribe. As an outcrossing crop with a short breeding history, domestication progress in cultivated NWR (cNWR) is ongoing, and seed shattering remains a major barrier to yield stability.
Reneth Millas, Lillian McGilp, Alan Mickelson, Maybell Banting, Claudia Castell‐Miller, Jennifer Kimball   +5 more
wiley   +1 more source

Adenosine‐to‐inosine editing of miR‐200b‐3p is associated with the progression of high‐grade serous ovarian cancer

Molecular Oncology, Volume 20, Issue 2, Page 409-427, February 2026.
A‐to‐I editing of miRNAs, particularly miR‐200b‐3p, contributes to HGSOC progression by enhancing cancer cell proliferation, migration and 3D growth. The edited form is linked to poorer patient survival and the identification of novel molecular targets.
Magdalena Niemira, Anna Skwarska, Karolina Chwialkowska, Agnieszka Ostrowska, Gabriela Sokolowska, Anna Zeller, Anna Erol, Andrzej Eljaszewicz, Bartosz Hanczaruk, Anna Michalska‐Falkowska, Agnieszka Tarasik, Joanna Reszec‐Gielazyn, Pawel Knapp, Marcin Moniuszko, Adam Kretowski   +14 more
wiley   +1 more source

Type I Interferon Gene Expression and Its Association With Ocular Involvement in Systemic Lupus Erythematosus

ACR Open Rheumatology, Volume 8, Issue 2, February 2026.
Objective Severalstudies have highlighted the role of Type I interferons (IFN‐I) in activating inflammatory pathways in lupus. However, no previous research focused on investigating the role of IFN‐I gene expression in the ophthalmologic involvement of systemic lupus erythematosus (SLE).
Raphael Teixeira Costa, Werbson Lima Guaraná, Braziliano Miguel da Silva Júnior, Jaciel Clementino, Luís Sousa Inês, Paula Sandrin‐Garcia, Thiago Sotero Fragoso   +6 more
wiley   +1 more source

Identification and validation of reference genes for quantitative gene expression analysis under 409 and 415 nm antimicrobial blue light treatment

Frontiers in Molecular Biosciences
IntroductionReverse transcription quantitative real-time polymerase chain reaction Q7 (RT‒qPCR) is a commonly used tool for gene expression quantification.
Beata Kruszewska-Naczk, Mariusz Grinholc, Aleksandra Rapacka-Zdonczyk   +2 more
doaj   +1 more source

SNRK3.15 Is a Crucial Component of the Sulfur Deprivation Response in Arabidopsis thaliana

Plant Direct, Volume 10, Issue 1, January 2026.
ABSTRACT Sulfate deprivation (–S) results in numerous metabolic and phenotypic alterations in plants. Kinases are often key players in transducing nutrient status signals to molecular components involved in metabolic and developmental program regulation, but despite the physiological importance of sulfur, to date, no signaling kinases have been ...
Anastasia Apodiakou, Elmien Heyneke, Saleh Alseekh, Pinnapat Pinsorn, Sabine Metzger, Stanislav Kopriva, Waltraud Schulze, Rainer Hoefgen, Sarah J. Whitcomb   +8 more
wiley   +1 more source

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Frontiers in Plant Science, 2017
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants.
Tao Li, Jing Wang, Miao Lu, Tianyi Zhang, Xinyun Qu, Zhezhi Wang   +5 more
doaj   +1 more source

Reference gene selection for quantitative real-time RT-PCR normalization in the half-smooth tongue sole (Cynoglossus semilaevis) at different developmental stages, in various tissue types and on exposure to chemicals. [PDF]

PLoS ONE, 2014
Quantitative real time RT-PCR has been described as the most sensitive method for the detection of low abundance mRNA. To date, no reference genes have been screened in the half-smooth tongue sole (Cynoglossus semilaevis).
Conghui Liu, Nian Xin, Yi Zhai, Liming Jiang, Jieming Zhai, Quanqi Zhang, Jie Qi   +6 more
doaj   +1 more source