Results 61 to 70 of about 294,484 (344)

Regulation of diverse nuclear shapes: pathways working independently, together

Communicative & Integrative Biology, 2021
Membrane-bound organelles provide physical and functional compartmentalization of biological processes in eukaryotic cells. The characteristic shape and internal organization of these organelles is determined by a combination of multiple internal and ...
Pallavi Deolal, Krishnaveni Mishra
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Mutations in multiple components of the nuclear pore complex cause nephrotic syndrome

Journal of Clinical Investigation, 2018
Steroid-resistant nephrotic syndrome (SRNS) almost invariably progresses to end-stage renal disease. Although more than 50 monogenic causes of SRNS have been described, a large proportion of SRNS remains unexplained.
D. Braun, S. Lovric, D. Schapiro, Ronen Schneider, Jonathan Marquez, M. Asif, M. Hussain, Ankana Daga, E. Widmeier, Jia Rao, S. Ashraf, Weizhen Tan, C. Lusk, Amy Kolb, Tilman Jobst-Schwan, J. Schmidt, Charlotte A. Hoogstraten, K. Eddy, Thomas M. Kitzler, S. Shril, A. Moawia, Kathrin Schrage, A. I. A. Khayyat, Jennifer A. Lawson, H. Gee, Jillian K. Warejko, Tobias Hermle, Amar J. Majmundar, Hannah Hugo, B. Budde, Susanne Motameny, J. Altmüller, A. A. Noegel, H. Fathy, Daniel P. Gale, S. Waseem, Ayaz Khan, L. Kerecuk, S. Hashmi, N. Mohebbi, R. Ettenger, E. Serdaroğlu, Khalid Alhasan, M. Hashem, S. Gonçalves, G. Ariceta, M. Ubetagoyena, Wolfram Antonin, S. Baig, F. Alkuraya, Q. Shen, Hong Xu, C. Antignac, R. Lifton, S. Mane, P. Nürnberg, M. Khokha, F. Hildebrandt   +57 more
semanticscholar   +1 more source

Expression of DNAJB12 or DNAJB14 causes coordinate invasion of the nucleus by membranes associated with a novel nuclear pore structure. [PDF]

PLoS ONE, 2014
DNAJB12 and DNAJB14 are transmembrane proteins in the endoplasmic reticulum (ER) that serve as co-chaperones for Hsc70/Hsp70 heat shock proteins. We demonstrate that over-expression of DNAJB12 or DNAJB14 causes the formation of elaborate membranous ...
Edward C Goodwin, Nasim Motamedi, Alex Lipovsky, Rubén Fernández-Busnadiego, Daniel DiMaio   +4 more
doaj   +1 more source

The Role of Nucleoporins in Cardiac Tissue Development and Disease

Frontiers in Bioscience-Landmark, 2023
Nuclear pore complexes (NPCs) are intricate intracellular structures composed of approximately 30 nuclear pore proteins (NUPs) that regulate the transport of materials between the nucleus and cytoplasm in eukaryotic cells.
Xiaocong Chen, Rui Shi, Yu Luo, Liang Xu
doaj   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

FEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang, Naoki Sunagawa, Toma Kashima, Kiyohiko Igarashi, Akimasa Miyanaga, Shinya Fushinobu   +5 more
wiley   +1 more source

DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex

Nature Communications, 2018
The nuclear pore complex (NPC) is the gatekeeper for nuclear transport in eukaryotic cells. A key component of the NPC is the central shaft lined with intrinsically disordered proteins (IDPs) known as FG-Nups, which control the selective molecular ...
Philip Ketterer, A. Ananth, D. S. Laman Trip, Ankur Mishra, E. Bertosin, M. Ganji, Jaco van der Torre, P. Onck, Hendrik Dietz, C. Dekker   +9 more
semanticscholar   +1 more source

Karyopherin binding interactions and nuclear import mechanism of nuclear pore complex protein Tpr

BMC Cell Biology, 2009
Background Tpr is a large protein with an extended coiled-coil domain that is localized within the nuclear basket of the nuclear pore complex. Previous studies 1 involving antibody microinjection into mammalian cells suggested a role for Tpr in nuclear ...
Frosst Phyllis D, Ben-Efraim Iris, Gerace Larry   +2 more
doaj   +1 more source