Results 61 to 70 of about 456,044 (305)
Nuclear transport and transcriptional regulation [PDF]
FEBS Letters, 1999 Studies over the past 10 years have provided major insights into the molecular mechanisms responsible for active transport of macromolecules in and out of the nucleus. Nucleocytoplasmic transport pathways correspond to active and signal‐mediated processes that involve substrates, adaptors and receptors.
Turpin, Pierre +2 more
openaire +2 more sources
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Nuclear Import of Hepatitis B Virus Capsids and Genome
Viruses, 2017 Hepatitis B virus (HBV) is an enveloped pararetrovirus with a DNA genome, which is found in an up to 36 nm-measuring capsid. Replication of the genome occurs via an RNA intermediate, which is synthesized in the nucleus.
Lara Gallucci, Michael Kann
doaj +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Nuclear Transport: Run by Ran? [PDF]
The American Journal of Human Genetics, 1998 A distinguishing feature of eukaryotic cells is the compartmentalization of their DNA within the nucleus. The sequestration of the genetic material away from the translational machinery and cytosolic proteins has at least two obvious but important implications: First, there must be a mechanism whereby the separate identities of the nucleus and cytosol ...
Dasso, Mary, Pu, Robert T.
openaire +2 more sources
Transport of proteins into mitochondria [PDF]
, 1985 Translocational intermediates of precursor proteins of ATPase F1β subunit and cytochrome c1 across mitochondrial membranes were analyzed using two different approaches, transport at low temperature and transport after binding of precursor proteins to ...
Schleyer, Manfred, Neupert, Walter
core +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Nuclear transport: Randy couples [PDF]
Current Biology, 1999 The recently solved structures of the Ran GTPase with a Ran-binding domain and with karyopherin-beta2 have revealed unusually tight embraces that provide important insights into the mechanism of nuclear transport and the many ways in which common protein folds are adapted to perform very different functions.
openaire +2 more sources
, 2012 The aim of the present study was to investigate if flumazenil blood-brain barrier transport and binding to the benzodiazepine site on the γ-aminobutyric acid A (GABA(A)) receptor complex is altered in an experimental model of epilepsy and subsequently to
Eriksson, P.A.J. +15 more
core +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source