Results 31 to 40 of about 249,615 (265)

Mild Heat Stress Alters the Physical State and Structure of Membranes in Triacylglycerol-Deficient Fission Yeast, Schizosaccharomyces pombe

Cells
We investigated whether the elimination of two major enzymes responsible for triacylglycerol synthesis altered the structure and physical state of organelle membranes under mild heat shock conditions in the fission yeast, Schizosaccharomyces pombe.
Péter Gudmann, Imre Gombos, Mária Péter, Gábor Balogh, Zsolt Török, László Vígh, Attila Glatz   +6 more
doaj   +1 more source

Mechanisms of organelle biogenesis govern stochastic fluctuations in organelle abundance

eLife, 2014
Fluctuations in organelle abundance can profoundly limit the precision of cell biological processes from secretion to metabolism. We modeled the dynamics of organelle biogenesis and predicted that organelle abundance fluctuations depend strongly on the ...
Shankar Mukherji, Erin K O'Shea
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Luteolin regulates the distribution and function of organelles by controlling SIRT1 activity during postovulatory oocyte aging

Frontiers in Nutrition, 2023
The quality of oocytes determines their development competence, which will be rapidly lost if the oocytes are not fertilized at the proper time after ovulation.
Xupeng Xing, Xupeng Xing, Jingfeng Peng, Jingfeng Peng, Jingyu Zhao, Jingyu Zhao, Ruoxi Shi, Ruoxi Shi, Caiqin Wang, Caiqin Wang, Zihan Zhang, Zihan Zhang, Zihan Wang, Zihan Wang, Zicong Li, Zicong Li, Zhenfang Wu, Zhenfang Wu   +17 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Biosensors Show the Pharmacokinetics of S-Ketamine in the Endoplasmic Reticulum

Frontiers in Cellular Neuroscience, 2019
The target for the “rapid” (<24 h) antidepressant effects of S-ketamine is unknown, vitiating programs to rationally develop more effective rapid antidepressants.
Kallol Bera, Aron Kamajaya, Amol V. Shivange, Anand K. Muthusamy, Anand K. Muthusamy, Aaron L. Nichols, Aaron L. Nichols, Philip M. Borden, Stephen Grant, Janice Jeon, Elaine Lin, Ishak Bishara, Theodore M. Chin, Bruce N. Cohen, Charlene H. Kim, Elizabeth K. Unger, Lin Tian, Jonathan S. Marvin, Loren L. Looger, Henry A. Lester   +19 more
doaj   +1 more source

AIEgen-Peptide Bioprobes for the Imaging of Organelles

Biosensors, 2022
Organelles are important subsystems of cells. The damage and inactivation of organelles are closely related to the occurrence of diseases. Organelles’ functional activity can be observed by fluorescence molecular tools.
Bochao Chen, Haotong Yuan, Wei Zhang, Jingjing Hu, Xiaoding Lou, Fan Xia   +5 more
doaj   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source

Loss of the Conserved Alveolate Kinase MAPK2 Decouples Toxoplasma Cell Growth from Cell Division

mBio, 2020
Mitogen-activated protein kinases (MAPKs) are a conserved family of protein kinases that regulate signal transduction, proliferation, and development throughout eukaryotes. The apicomplexan parasite Toxoplasma gondii expresses three MAPKs.
Xiaoyu Hu, William J. O’Shaughnessy, Tsebaot G. Beraki, Michael L. Reese   +3 more
doaj   +1 more source