HOPX regulates the development and lineage progression of human stem cell-derived outer radial glia-like cells [PDF]
Stem Cell ReportsSummary: Outer radial glia (oRG) are abundant in the human fetal cortex but rare in rodents, yet their molecular regulation remains poorly understood. Although the homeodomain-only protein X ( HOPX) is a well-established oRG marker, its functional role ...
Xiao-Xue Dong +18 more
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Cerebral Cortex, 2021 Abstract The neocortex, the center for higher brain function, emerged in mammals and expanded in the course of evolution. The expansion of outer radial glia (oRGs) and intermediate progenitor cells (IPCs) plays key roles in the expansion and consequential folding of the neocortex.
Shirui Hou +5 more
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A kinase-independent function of cyclin-dependent kinase 6 promotes outer radial glia expansion and neocortical folding [PDF]
Proceedings of the National Academy of Sciences, 2022 Abstract The neocortex, the center for higher brain function, first emerged in mammals and has become massively expanded and folded in humans, constituting almost half the volume of the human brain. Primary microcephaly, a developmental disorder in which the brain is smaller than normal at birth, mainly results from the number of ...
Lei Wang +15 more
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Development, 2023 ABSTRACT The definition of molecular and cellular mechanisms contributing to brain ontogenetic trajectories is essential to investigate the evolution of our species. Yet their functional dissection at an appropriate level of granularity remains challenging.
Juan Moriano +4 more
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Diverse Behaviors of Outer Radial Glia in Developing Ferret and Human Cortex [PDF]
The Journal of Neuroscience, 2014 The dramatic increase in neocortical size and folding during mammalian brain evolution has been attributed to the elaboration of the subventricular zone (SVZ) and the associated increase in neural progenitors. However, recent studies have shown that SVZ size and the abundance of resident progenitors do not directly predict cortical topography ...
Gertz, Caitlyn C +4 more
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Protocol for generating human cortical organoids enriched in outer radial glia by guided differentiation [PDF]
STAR ProtocolsThe generation of human pluripotent stem cell (hPSC)-derived brain organoids is continuously refined, enhancing their reproducibility and complexity. Here, we present a guided differentiation protocol for generating cortical forebrain organoids and cortico-pericyte (CP)assembloids composed of a robust outer radial glia (oRG) population and an expanded ...
Raffaele Luongo +4 more
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A Müller Glia‐Retinal Pigment Epithelium Apical Network Surrounding Cone Outer Segments in Fish and Mouse [PDF]
Glia, Volume 74, Issue 12, December 2026.Müller glia and the retinal pigment epithelium interact around cone outer segment in the subretinal space of both zebrafish and mouse. The contacts are established early during development but subsequent to outer segment emergence. ABSTRACT Homeostasis of the vertebrate retina critically depends on two non‐neuronal cell populations, Müller glia and the
Maria Sharkova +6 more
wiley +2 more sources
Molecular signatures and lineage diversification of neurogenic and gliogenic radial glia in the gyrencephalic ferret cortex [PDF]
Communications BiologyHuman exceptional cognition stems from evolutionarily derived cortical adaptations that drive expansive neurogenesis. In this study, we employ the gyrencephalic ferret model to systematically characterize the molecular profiles and lineage dynamics of ...
Jialin Li +21 more
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Molecular Control of Neural Stem Cell Quiescence in the Adult Zebrafish Telencephalon: Established Mechanisms and an Integrative Hypothesis [PDF]
Glia, Volume 74, Issue 12, December 2026.Adult zebrafish radial glia quiescence is controlled by at least four mechanisms: Notch3, BMP‐Id1, Nr2f1b and Midkine‐a. We propose these act as partially redundant inputs, biased along the antero‐posterior axis that set quiescence depth. ABSTRACT The zebrafish telencephalon is an excellent model for adult neurogenesis under physiological and ...
Mathilde Hoareau +2 more
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CellCover defines marker gene panels capturing developmental progression in neocortical neural stem cell identity [PDF]
eLifeDefining cell classes is central to the analysis of growing single-cell RNA sequencing (scRNA-seq) atlases. Marker genes are most often identified by differential expression (DE) methods that assess genes one at a time, ignoring the redundancy and ...
Lanlan Ji +7 more
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