Results 11 to 20 of about 29,607 (257)
Automated environmental metagenomics using Oxford nanopore sequencing. [PDF]
BMC GenomicsAbstract Long-read sequencing has revolutionised metagenomics through improved metagenome assembly, taxonomic classification and functional characterisation. Automation can enhance the throughput, reproducibility, and accuracy of library preparation.
Child HT +5 more
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A comparison of Oxford nanopore library strategies for bacterial genomics [PDF]
BMC Genomics, 2023 Abstract Background Oxford nanopore Technologies (ONT) provides three main library preparation strategies to sequence bacterial genomes. These include tagmentation (TAG), ligation (LIG) and amplification (PCR). Despite ONT’s recommendations, making an informed decision for preparation choice remains difficult without a ...
Sauvage, Thomas +2 more
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Optimized use of Oxford Nanopore flowcells for hybrid assemblies [PDF]
Microbial Genomics, 2020 Hybrid assemblies are highly valuable for studies of Enterobacteriaceae due to their ability to fully resolve the structure of mobile genetic elements, such as plasmids, which are involved in the ...
Samuel Lipworth +14 more
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Application of Oxford Nanopore Technology to Plant Virus Detection [PDF]
Viruses, 2021 The adoption of Oxford Nanopore Technologies (ONT) sequencing as a tool in plant virology has been relatively slow despite its promise in more recent years to yield large quantities of long nucleotide sequences in real time without the need for prior amplification.
Lia W. Liefting +2 more
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Oxford Nanopore sequencing: new opportunities for plant genomics? [PDF]
Journal of Experimental Botany, 2020 Abstract DNA sequencing was dominated by Sanger’s chain termination method until the mid-2000s, when it was progressively supplanted by new sequencing technologies that can generate much larger quantities of data in a shorter time. At the forefront of these developments, long-read sequencing technologies (third-generation sequencing) can
Dumschott, Kathryn +4 more
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Prowler: A novel trimming algorithm for Oxford Nanopore sequence data [PDF]
Bioinformatics, 2021 Abstract Motivation Quality control (QC) tools are critical in DNA sequencing analysis because they increase the accuracy of sequence alignments and thus the reliability of results. Oxford Nanopore Technologies (ONT) QC is currently rudimentary, generally based on whole read average quality.
Simon Lee +3 more
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The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community
Genome Biology, 2016 Nanopore DNA strand sequencing has emerged as a competitive, portable technology. Reads exceeding 150 kilobases have been achieved, as have in-field detection and analysis of clinical pathogens. We summarize key technical features of the Oxford Nanopore MinION, the dominant platform currently available.
Miten Jain +3 more
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Oxford nanopore sequencing in clinical microbiology and infection diagnostics
Briefings in Bioinformatics, 2021 Abstract Extended turnaround times and large economic costs hinder the usage of currently applied screening methods for bacterial pathogen identification (ID) and antimicrobial susceptibility testing. This review provides an overview of current detection methods and their usage in a clinical setting.
Dropen Sheka +2 more
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Recovery of small plasmid sequences via Oxford Nanopore sequencing [PDF]
Microbial Genomics, 2021 Oxford Nanopore Technologies (ONT) sequencing platforms currently offer two approaches to whole-genome native-DNA library preparation: ligation and rapid. In this study, we compared these two approaches for bacterial whole-genome sequencing, with a specific aim of assessing their ability to recover small plasmid sequences.
Ryan R. Wick +3 more
openaire +3 more sources
A chromosome-level genome assembly of the jade perch (Scortum barcoo)
Scientific Data, 2022 Measurement(s) whole genome sequencing Technology Type(s) Illumina Sequencing • Oxford Nanopore ...
Yishan Lu +11 more
doaj +1 more source