Results 31 to 40 of about 29,607 (257)

An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations

Micromachines, 2023
There has been significant progress made in the field of nanopore biosensor development and sequencing applications, which address previous limitations that restricted widespread nanopore use.
Morgan MacKenzie, Christos Argyropoulos
doaj   +1 more source

Nanopore sequencing technology and its application in plant virus diagnostics

Frontiers in Microbiology, 2022
Plant viruses threaten crop yield and quality; thus, efficient and accurate pathogen diagnostics are critical for crop disease management and control. Recent advances in sequencing technology have revolutionized plant virus research.
Kai Sun, Yi Liu, Xin Zhou, Chuanlin Yin, Pengjun Zhang, Qianqian Yang, Lingfeng Mao, Xuping Shentu, Xiaoping Yu   +8 more
doaj   +1 more source

Absence of evidence is not evidence of absence: Nanopore sequencing and complete assembly of the European lobster (Homarus gammarus) mitogenome uncovers the missing nad2 and a new major gene cluster duplication

BMC Genomics, 2019
Background The recently published complete mitogenome of the European lobster (Homarus gammarus) that was generated using long-range PCR exhibits unusual gene composition (missing nad2) and gene rearrangements among decapod crustaceans with strong ...
Han Ming Gan, Frederic Grandjean, Tom L. Jenkins, Christopher Mervyn Austin   +3 more
doaj   +1 more source

NanoSplicer: accurate identification of splice junctions using Oxford Nanopore sequencing [PDF]

Bioinformatics, 2021
Abstract Motivation Long-read sequencing methods have considerable advantages for characterizing RNA isoforms. Oxford Nanopore sequencing records changes in electrical current when nucleic acid traverses through a pore.
Yupei You, Michael B. Clark, Heejung Shim   +2 more
openaire   +3 more sources

The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota cultures

IMA Fungus, 2020
We formed the Collection of Zoosporic Eufungi at the University of Michigan (CZEUM) in 2018 as a cryopreserved fungal collection consolidating the University of Maine Culture Collection (UMCC, or JEL), the University of Alabama Chytrid Culture Collection
D. Rabern Simmons, Anne E. Bonds, Buck T. Castillo, Rebecca A. Clemons, Alex D. Glasco, Jillian M. Myers, Natasha Thapa, Peter M. Letcher, Martha J. Powell, Joyce E. Longcore, Timothy Y. James   +10 more
doaj   +1 more source

Chromosomal-level genome assembly of potato tuberworm, Phthorimaea operculella: a pest of solanaceous crops

Scientific Data, 2022
Measurement(s) whole genome sequencing Technology Type(s) Illumina NovaSeq. 6000 • Oxford Nanopore Sequencing Sample Characteristic - Organism Phthorimaea operculella Sample Characteristic - Location Yunnan Province, Southwest ...
Mengdi Zhang, Xinyue Cheng, Runmao Lin, Bingyan Xie, Ralf Nauen, Silvia I. Rondon, Jorge A. Zavala, Subba Reddy Palli, Suhua Li, Xingyao Xiong, Wenwu Zhou, Yulin Gao   +11 more
doaj   +1 more source

Single‐cell DNA methylation profiling: Technologies, computation, and applications in precision oncology

Molecular Oncology, EarlyView.
Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley   +1 more source

High resolution long-read telomere sequencing reveals dynamic mechanisms in aging and cancer

Nature Communications
Telomeres are the protective nucleoprotein structures at the end of linear eukaryotic chromosomes. Telomeres’ repetitive nature and length have traditionally challenged the precise assessment of the composition and length of individual human telomeres ...
Tobias T. Schmidt, Carly Tyer, Preeyesh Rughani, Candy Haggblom, Jeffrey R. Jones, Xiaoguang Dai, Kelly A. Frazer, Fred H. Gage, Sissel Juul, Scott Hickey, Jan Karlseder   +10 more
doaj   +1 more source

Detection of DNA base modifications by deep recurrent neural network on Oxford Nanopore sequencing data

Nature Communications, 2019
DNA modification generates unique electric signals in Oxford Nanopore sequencing data but the signals can be complicated to decipher. Here, the authors develop a deep learning framework, DeepMod, to detect DNA base modifications including 5mC and 6mA ...
Qian Liu, Li Fang, Guoliang Yu, Depeng Wang, Chuan-Le Xiao, Kai Wang   +5 more
doaj   +1 more source

Transformer‐based DNA methylation detection on ionic signals from Oxford Nanopore sequencing data

Quantitative Biology, 2023
Transformer is an algorithm that adopts self‐attention architecture in the neural networks and has been widely used in natural language processing. In the current study, we apply Transformer architecture to detect DNA methylation on ionic signals from ...
Xiuquan Wang, Mian Umair Ahsan, Yunyun Zhou, Kai Wang   +3 more
doaj   +1 more source