Results 51 to 60 of about 29,607 (257)

Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Aiswarya Saravanan, Jordan Safran, Anna Loughman, Grace Lin, Shivanshi Vaid, Dana Allababidi, Peter M. Tessier, Louis T. Dang   +7 more
wiley   +1 more source

Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives

Advanced Materials, EarlyView.
Kelvin probe force microscopy (KPFM) enables the nanoscale mapping of electrostatic surface potentials. While widely applied in materials science, its use in biological systems remains emerging. This review presents recent advances in KPFM applied to biological samples and provides a critical perspective on current limitations and future directions for
Ehsan Rahimi, Mario Palacios‐Corella, Arjan Mol, Salvador Pané, Josep Puigmartí‐Luis   +4 more
wiley   +1 more source

Comparison Of Oxford Nanopore Basecalling Tools

, 2017
This repository uses a bacterial genome to assess the read accuracy and consensus sequence accuracy for Oxford Nanopore Technologies (ONT) basecallers. Albacore v2.1.10, Guppy v0.3.0 and Scrappie raw v1.3.0 (all developed by ONT) were the best performers for read accuracy, and Chiron v0.3 produced the best assemblies.
Wick, Ryan R, Judd, Louise M, Holt, Kathryn E   +2 more
openaire   +5 more sources

An Ion‐Based Strategy Toward Synergistic Surface Functionalization Combining the Osteogenic Properties and NIR‐Mediated Antibacterial Activity of PEEK

Advanced Materials, EarlyView.
A multifunctional bioactive PEEK‐based implant (Mn@p/MBG‐SPEEK) is engineered to combat implant‐associated infections while promoting osteogenesis. The hybrid surface activates PI3K/Akt/mTOR and AP‐1 signaling pathways for osteogenic differentiation, while Mn coordination induces electronic coupling that narrows the HOMO‐LUMO energy gap and enhances ...
Zhiyan Xu, Ondřej Adam, Marek Doubrava, Ana M. Beltrán, Ivo Dlouhý, Xin Liu, Baiyan Sui, Aldo R. Boccaccini   +7 more
wiley   +1 more source

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

Nature Communications
Abundant cellular transcripts occupy most of the sequencing reads in the transcriptome, making it challenging to assay for low-abundant transcripts. Here, we utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and ...
Jiaxu Wang, Lin Yang, Anthony Cheng, Cheng-Yong Tham, Wenting Tan, Jefferson Darmawan, Paola Florez de Sessions, Yue Wan   +7 more
doaj   +1 more source

Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing

BMC Genomics, 2019
Background Short-read sequencing technologies have made microbial genome sequencing cheap and accessible. However, closing genomes is often costly and assembling short reads from genomes that are repetitive and/or have extreme %GC content remains ...
Sarah Goldstein, Lidia Beka, Joerg Graf, Jonathan L. Klassen   +3 more
doaj   +1 more source

Aggregation‐Controlled Vat Dyeing of Meta‐Aramid Fibers: Mechanistic Insights and Suppression of Surface Dye Deposition

Advanced Materials Interfaces, EarlyView.
Supramolecular aggregation governs the interfacial adsorption, diffusion, and fixation of anthraquinone‐based vat dyes in meta‐aramid fibers. By correlating reduction chemistry, aggregate formation, and fiber microstructure, a multiscale mechanism for aggregation‐controlled dyeing is established, providing an effective strategy to suppress surface dye ...
Jisiyuan Qin, Yahui Xia, Shiqi Hu, Yongmin Wu, Mohammad Raza Miah, Ruizhe Lu, Yunhan Tang, Genyang Cao, Sakil Mahmud   +8 more
wiley   +1 more source

Whole genome sequencing in animal health: applications, challenges, and future directions

BMC Veterinary Research
WGS is revolutionizing animal health diagnostics, offering unprecedented opportunities for pathogen detection and biomarker identification, while offering increased depth of data, in comparison to more targeted approaches such as amplicon sequencing ...
Johannes Charlier, Alistair Antonopoulos, Bart J.G. Broeckx, Aaron Pomerantz, Veronica L Fowler, Volodimir Olexiouk, Sebastiaan Theuns, Sven Arnouts   +7 more
doaj   +1 more source

Availability of Nanopore sequences in the genome taxonomy for Vibrionaceae systematics: Rumoiensis clade species as a test case [PDF]

PeerJ, 2018
Whole genome sequence comparisons have become essential for establishing a robust scheme in bacterial taxonomy. To generalize this genome-based taxonomy, fast, reliable, and cost-effective genome sequencing methodologies are required.
Mami Tanaka, Sayaka Mino, Yoshitoshi Ogura, Tetsuya Hayashi, Tomoo Sawabe   +4 more
doaj   +2 more sources

Flexible Implantable Silicon Carbide Multi‐Electrode Array (MEA) for Cell Multichannel Recording and Cell Ablation

Advanced Materials Technologies, EarlyView.
Flexible silicon carbide (SiC) microelectrode arrays enable high‐fidelity, multichannel cell extracellular recording and precise localized ablation. SiC has been extensively evaluated to persist long‐term in chronic physiological conditions while remaining robust, with excellent electrical and electrochemical stability.
Minh Anh Huynh, Matthew Barton, Sharda Yadav, Tuan‐Khoa Nguyen, Nam‐Trung Nguyen   +4 more
wiley   +1 more source