Results 51 to 60 of about 33,843 (263)

The RNA‐Binding Protein PARN Remodeled 3′ UTR Structure Defines Poly(A)‐Loading Sites to Mediate Immunoglobulin Homeostasis

Advanced Science, EarlyView.
ABSTRACT Class Switch Recombination (CSR) is essential for generating high‐affinity antibody isotypes from IgM during adaptive humoral responses. Despite well‐established roles for various transcription factors, whether CSR is subject to dedicated post‐transcriptional control represents a significant gap in knowledge.
Siyuan Sun, Chen Yang, Xiaoyu Wang, Naijing Hu, Shengyao Zhang, Xinchun Li, Yu Chen, Jianan Zhai, Xiangfeng Tang, Wei Lu, Chenxu Lu, Changchang Cao, Weiru Yu, Wenhao Wu, Xiaonan Nie, Fengchao Wang, Bing Fang, Fazheng Ren, Yixuan Li, Juan Chen   +19 more
wiley   +1 more source

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

Nature Communications
Abundant cellular transcripts occupy most of the sequencing reads in the transcriptome, making it challenging to assay for low-abundant transcripts. Here, we utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and ...
Jiaxu Wang, Lin Yang, Anthony Cheng, Cheng-Yong Tham, Wenting Tan, Jefferson Darmawan, Paola Florez de Sessions, Yue Wan   +7 more
doaj   +1 more source

Availability of Nanopore sequences in the genome taxonomy for Vibrionaceae systematics: Rumoiensis clade species as a test case [PDF]

PeerJ, 2018
Whole genome sequence comparisons have become essential for establishing a robust scheme in bacterial taxonomy. To generalize this genome-based taxonomy, fast, reliable, and cost-effective genome sequencing methodologies are required.
Mami Tanaka, Sayaka Mino, Yoshitoshi Ogura, Tetsuya Hayashi, Tomoo Sawabe   +4 more
doaj   +2 more sources

Evaluating nanopore sequencing data processing pipelines for structure variation identification, supplemental data

, 2019
nanopore_03262018.fastq.gz Description: DNA sample of individual NS12911 was sequenced by Oxford Nanopore (ONT) MinION using flowcell FLO-MIN106D. The library was constructed using ONT 1D Genomic DNA by ligation protocol.

core   +1 more source

T2T Genome Assembly and Multi‐Omics Data Reveal Terrestrial Adaptation and Mucus Biosynthesis in Tropical Leatherleaf Slug (Laevicaulis alte)

Advanced Science, EarlyView.
A gap‐free genome assembly and multi‐omics comparison of the terrestrial slug Laevichaulis alte with an aquatic relative reveal that expansion of the VEGF family orchestrates mucus production, lipid metabolism, and immune defense—highlighting key molecular innovations for conquering life on land.
Gang Wang, Shaofang He, Zhongkai Wang, Aobo Pang, Xiaoli Sun, Rongchen Liu, Fang Wang, Sisi Chen, Zhijuan Bian, Dongcheng Wei, Liuhan Wu, Siyao Zhao, Qiuting Ji, Yiqi Sun, Niying Sun, Yushinta Fujaya, Boping Tang, Kianann Tan, Daizhen Zhang, Lianfu Chen   +19 more
wiley   +1 more source

Whole genome sequencing in animal health: applications, challenges, and future directions

BMC Veterinary Research
WGS is revolutionizing animal health diagnostics, offering unprecedented opportunities for pathogen detection and biomarker identification, while offering increased depth of data, in comparison to more targeted approaches such as amplicon sequencing ...
Johannes Charlier, Alistair Antonopoulos, Bart J.G. Broeckx, Aaron Pomerantz, Veronica L Fowler, Volodimir Olexiouk, Sebastiaan Theuns, Sven Arnouts   +7 more
doaj   +1 more source

Nanopore Sequencing in Blood Diseases: A Wide Range of Opportunities

Frontiers in Genetics, 2020
The molecular pathogenesis of hematological diseases is often driven by genetic and epigenetic alterations. Next-generation sequencing has considerably increased our genomic knowledge of these disorders becoming ever more widespread in clinical practice.
Crescenzio Francesco Minervini, Cosimo Cumbo, Paola Orsini, Luisa Anelli, Antonella Zagaria, Giorgina Specchia, Francesco Albano   +6 more
doaj   +1 more source

DeepNano: Deep recurrent neural networks for base calling in MinION nanopore reads. [PDF]

PLoS ONE, 2017
The MinION device by Oxford Nanopore produces very long reads (reads over 100 kBp were reported); however it suffers from high sequencing error rate.
Vladimír Boža, Broňa Brejová, Tomáš Vinař   +2 more
doaj   +1 more source

Implementation of a flexible Oxford Nanopore sequencing platform for microbial genomics [PDF]

, 2021
Oxford Nanopore sequencing technology is slowly revolutionising the entire microbiology field. Its ease of use and cost effective approaches coupled with long reads sequencing represent an essential and powerful tool.
PINZAUTI, DAVID
core   +1 more source

Sustainable Carbon Fibers Enable Stable Long‐Term Lithium Metal Deposition for Prospective Zero‐Excess Lithium Metal Batteries

Advanced Energy Materials, EarlyView.
This work presents lightweight, lignin‐derived carbon fiber current collectors that enable controlled lithium deposition. Structural defects and intermediate‐sized pores stabilize pre‐nucleation quasi‐metallic lithium clusters, promoting uniform lithium plating and stripping.
Samantha L. S. Southern, Jonathon Cottom, Jana B. Fritzke, Emma N. Antonio, Zhenyu Guo, Bhavini Patel, Romain Tort, Ifan E. L. Stephens, Camilla Di Mino, Clare P. Grey, Rhodri Jervis, Emilia Olsson, Heather Au, Maria‐Magdalena Titirici   +13 more
wiley   +1 more source