Results 61 to 70 of about 5,800,592 (273)

DNA origami nanopores: an emerging tool in biomedicinet

open access: yes, 2013
Editorial.The authors acknowledge support from an ERC starting grant and from Oxford Nanopore Technologies® (www.nanoporetech.com).Peer ...
Hernández-Ainsa, Silvia   +1 more
core   +1 more source

Revolutionising Agricultural Sustainability: New ‘Furrow Tillage’ can Mitigate Short‐Term Soil‐to‐Atmosphere CO2 Flux and Promote Soil‐Plant‐Microbe Health

open access: yesAdvanced Science, EarlyView.
Furrow tillage resolves the conventional‐vs.‐no‐tillage trade‐off by simultaneously cutting CO2 efflux to 2.0–3.0 g C m−2 d−1 and unlocking high nutrient availability for the rice rhizosphere. This scalable agronomic solution strengthens soil health, enhances plant physiology, reshapes microbial metabolism, and shifts paddy systems toward a net ...
Arnab Majumdar   +10 more
wiley   +1 more source

Comparison Of Oxford Nanopore Basecalling Tools

open access: yes, 2017
This repository uses a bacterial genome to assess the read accuracy and consensus sequence accuracy for Oxford Nanopore Technologies (ONT) basecallers. Albacore v2.1.10, Guppy v0.3.0 and Scrappie raw v1.3.0 (all developed by ONT) were the best performers for read accuracy, and Chiron v0.3 produced the best assemblies.
Wick, Ryan R   +2 more
openaire   +5 more sources

A Personalized Haplotype‐Resolved Near‐Gapless Genome Framework for Somatic Variant Discovery in Hepatocellular Carcinoma

open access: yesAdvanced Science, EarlyView.
A patient‐specific, haplotype‐resolved genome framework improves detection and interpretation of somatic variants in hepatocellular carcinoma. Using multi‐platform sequencing, the personalized assembly resolves complex regions including centromeres and MHC/HLA loci, enhances structural variant discovery, and links regulatory alterations to allele ...
Jiazheng Lin   +17 more
wiley   +1 more source

Hybrid genome assembly and phenotypic assays reveal carbohydrate metabolism diversity in Lacticaseibacillus strains

open access: yesApplied Microbiology and Biotechnology
Investigation of carbohydrate metabolism in lactic acid bacteria is essential for the rational selection of strains for fermentation processes, particularly in emerging applications involving non-conventional substrates or building of synthetic microbial
Emanuele Della Monica   +7 more
doaj   +1 more source

Performance of neural network basecalling tools for Oxford Nanopore sequencing [PDF]

open access: yesGenome Biology, 2019
Abstract Basecalling, the computational process of translating raw electrical signal to nucleotide sequence, is of critical importance to the sequencing platforms produced by Oxford Nanopore Technologies (ONT). Here we examine the performance of different basecalling tools, looking at accuracy at the level of bases within individual ...
Wick, Ryan R.   +2 more
openaire   +4 more sources

Atmospheric Water Harvesting Through Polymer Hydrogel Sorbents: Materials Chemistry, Design Strategies, and Applications

open access: yesAdvanced Science, EarlyView.
Polymer‐based hydrogels have emerged as promising materials for atmospheric water harvesting (AWH) due to their high water affinity, tunable network structure, and excellent moisture adsorption‐desorption performance. Functional hydrogel systems enable efficient water capture and controlled release under low‐energy conditions, offering a sustainable ...
Swagata Datta   +6 more
wiley   +1 more source

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

open access: yesNature Communications
Abundant cellular transcripts occupy most of the sequencing reads in the transcriptome, making it challenging to assay for low-abundant transcripts. Here, we utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and ...
Jiaxu Wang   +7 more
doaj   +1 more source

Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing

open access: yesBMC Genomics, 2019
Background Short-read sequencing technologies have made microbial genome sequencing cheap and accessible. However, closing genomes is often costly and assembling short reads from genomes that are repetitive and/or have extreme %GC content remains ...
Sarah Goldstein   +3 more
doaj   +1 more source

Optimized Cas9‐Enriched Nanopore Sequencing and Analysis Workflow for Clinical Diagnosis of Repeat Expansion Disorders

open access: yesAdvanced Science, EarlyView.
An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Seungbok Lee   +11 more
wiley   +1 more source

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