Unusual fragmentation of derivatized cysteine‐containing peptides [PDF]
Rapid Communications in Mass Spectrometry, 2018 RationaleModification of cysteines by aminoethylation results in side chains similar to those of lysine. Trypsin cleaves at this modified residue and this labeling method can facilitate the analysis of proteins, specifically antibodies. In this work, the ability to identify peptides containing aminoethylated cysteines is investigated through digestion,
Nick Degraan‐Weber +2 more
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Database Search Strategies for Proteomic Data Sets Generated by Electron Capture Dissociation Mass Spectrometry [PDF]
, 2009 Large data sets of electron capture dissociation (ECD) mass spectra from proteomic experiments are rich in information; however, extracting that information in an optimal manner is not straightforward.
Beausoleil S. A. +17 more
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Identification of Viral Peptide Fragments for Vaccine Development [PDF]
, 2009 We report a simple method for identifying foldable viral surface protein fragments in a random but systematic manner. The method involves digestion and reassembly of a target gene to generate a pool of smaller DNA fragments with random ends but controllable lengths, followed by screening for foldable fragments using green fluorescent protein (GFP) as a
Yong Chen, Zhanglin Lin, Shuang Li
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Probing the mechanism of electron capture and electron transfer dissociation using tags with variable electron affinity [PDF]
, 2009 Electron capture dissociation (ECD) and electron transfer dissociation (ETD) of doubly protonated electron affinity (EA)-tuned peptides were studied to further illuminate the mechanism of these processes. The model peptide FQpSEEQQQTEDELQDK, containing a
Beauchamp, J. L. +5 more
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Peptide fragmentation induced by radicals at atmospheric pressure [PDF]
Journal of Mass Spectrometry, 2008 AbstractA novel ion dissociation technique, which is capable of providing an efficient fragmentation of peptides at essential atmospheric pressure conditions, is developed. The fragmentation patterns observed often contain c‐type fragments that are specific to electron capture dissociation/electron transfer dissociation (ECD/ETD), along with the y‐/b ...
Andrey N. Vilkov +2 more
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Selection of gonadotrophin surge attenuating factor phage antibodies by bioassay [PDF]
, 2005 Peer reviewedPublisher ...
Fowler, Paul Alfred Francois +3 more
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A neutrophil-dependent pathway for the generation of a neutral peptide mediator: partial characterization of components and control by alpha-1-antitrypsin. [PDF]
, 1974 A biologically active neutral peptide mediator is cleaved from a plasma protein substrate by an alpha-1-antitrypsin-inhibitable serine protease apparently residing on the membrane of the human neutrophil. The peptide mediator has an approximate mol wt of
Austen, KF, Goetzl, EJ, Wintroub, BU
core
Comparative Analysis of the Major Polypeptides from Liver Gap Junctions and Lens Fiber Junctions [PDF]
, 1982 Gap junctions from rat liver and fiber junctions from bovine lens have similar septilaminar profiles when examined by thin-section electron microscopy and differ only slightly with respect to the packing of intramembrane particles in freeze-fracture ...
Anderson, David J. +3 more
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Direct regulation of Treslin by cyclin-dependent kinase is essential for the onset of DNA replication [PDF]
, 2011 Treslin, a TopBP1-interacting protein, is necessary for deoxyribonucleic acid (DNA) replication in vertebrates. Association between Treslin and TopBP1 requires cyclin-dependent kinase (Cdk) activity in Xenopus laevis egg extracts.
Dunphy, William G. +3 more
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A nomenclature system for labeling cyclic peptide fragments
Journal of the American Society for Mass Spectrometry, 1999 A nomenclature system for labeling fragment ions of cyclic peptides is proposed. A fragment ion is labeled with a four-part descriptor for the general formula xnJZ, where x is the designation for the ion (e.g., lower case a or b, as are used for peptide fragments) and n is the number of amino acid residues in the ion.
Lambert C. M. Ngoka, Michael L. Gross
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