Results 91 to 100 of about 83,579 (259)

Developmentally Guided Differentiation of Ventral Midbrain Dopaminergic Cells Validated in Proteopathy‐Based Parkinson's Disease Models

Advanced Science, EarlyView.
A developmentally guided protocol reproducibly specifies EN1‐positive ventral midbrain dopaminergic (vmDA) progenitors from human pluripotent stem cells and yields functional vmDA neurons. Optogenetic induction of α‐synuclein aggregation elicits selective degeneration of TH‐positive neurons in 2D cultures and 3D neurospheroids, while transplanted ...
Heechang Moon, Heejeong Kim, Elliot H. Lee, Hanseul Kim, Ji Hun Kim, Huisu Jeong, Jiwon Cheon, Ga Ram Jeong, Byoung Dae Lee, Jae‐Won Cho, Soo‐Jin Oh, Yunjong Lee, Yohan Oh   +12 more
wiley   +1 more source

Regulators of pluripotency and their implications in regenerative medicine

, 2015
Ahmed El-Badawy, Nagwa El-Badri Center of Excellence for Stem Cells and Regenerative Medicine, Zewail City of Science and Technology, Giza, Egypt Abstract: The ultimate goal of regenerative medicine is to replace damaged tissues with new functioning ...
El-Badawy A, El-Badri N
core  

Pluripotency of Li-iPSCs.

, 2019
(A) Surface pluripotency marker TRA-1-60 specific or isotype IgG life immunofluorescence analysis of Li-iPSC colonies derived from patients C101 and C496 (scale bar 250μm).
Dagmara Szkolnicka (5070317), Diego Calabrese (165146), David C. Hay (5070335), Guglielmo Roma (155263), Isabel Fofana (424772), Stefan Wieland (199656), Markus H. Heim (7325642), Sandro Nuciforo (728282), Tewis Bouwmeester (4352), Valentina Mele (1863244), Walter Carbone (3480254), Benedetta Campana (827810), Sebastian Bergling (785781), Jan Tchorz (151052)   +13 more
core   +1 more source

Mitochondrial Localization of Cx26 Promotes Colorectal Cancer Malignancy and Exposes a Fatal Vulnerability to Drug Targeting

Advanced Science, EarlyView.
Mitochondrial localization of Cx26 emerges as a double‐edged sword in colorectal cancer. PIMT‐mediated methylation of Cx26 promotes its mitochondrial localization and stability. Mitochondrial Cx26 then facilitates ER‐mitochondria communication and Ca2+ signaling, thereby promoting metastatic progression. Bortezomib exploits this malignant adaptation by
Jie Wang, Lu Chen, Jia Qin, Xiaoning Wang, Sunlan Ye, Ning An, Mei Bai, Yan Shen, Xuefeng Wu, Qiang Xu   +9 more
wiley   +1 more source

SEC Mediates m6A Deposition and Transcription Pause Release to Drive Cell Identity Transition

Advanced Science, EarlyView.
The SEC promotes cell identity transitions by facilitating transcription pause release at key identity‐associated genes, including Klf4 and Myc during iPSC reprogramming and Nes and Tubb3 during ESC‐to‐neuroectodermal differentiation. During early iPSC reprogramming, this function additionally involves cooperation with METTL3‐associated m6A regulation.
Zhijing Zhang, Jingyi Xu, Yonghong Wu, Yitong Qu, Xinglin Hu, Yuchen Sun, Yanshuang Wu, Zhonghua Liu, Jiaqiang Wang, Lei Lei   +9 more
wiley   +1 more source

Capturing Transitional Pluripotency through Proline Metabolism

, 2022
In this paper, we summarize the current knowledge of the role of proline metabolism in the control of the identity of Embryonic Stem Cells (ESCs). An imbalance in proline metabolism shifts mouse ESCs toward a stable naïve-to-primed intermediate state of ...
Cristina D’Aniello, De Cesare, Dario, Annalisa Fico, Minchiotti, Gabriella, D’Aniello, Cristina, Gabriella Minchiotti, Dario De Cesare, Eduardo Jorge Patriarca, Fico, Annalisa, Patriarca, Eduardo Jorge, Minchiotti G., D'aniello C., De Cesare D., Fico A., Patriarca E. J.   +14 more
core   +1 more source

One‐Step Induction of Oogonia‐Like and Pro‐Spermatogonia‐Like Cells From Porcine Pluripotent Stem Cells

Advanced Science, EarlyView.
This study established a one‐step method inducing pro‐spermatogonia‐like (activating SOX17) and oogonia‐like (activating SOX17/TFAP2C) cells from porcine pgEpiSCs with a NANOS3‐tdTomato reporter. EGF activates the core PGC program and supports survival; BMP4 further induces NANOS3 and DDX4 expression; IWR1 enhances PGCLC efficiency by suppressing ...
Pengcheng He, Liang Zhong, Tianzhi Chen, Yaxuan Yan, Xinze Chen, Runbo Li, Yingjie Wang, Yadi Teng, Minglei Zhi, Meng Wang, Jinying Zhang, Fan Zhao, Dengfeng Gao, Suying Cao, Jianyong Han   +14 more
wiley   +1 more source

Oct4 Distribution and Level in Mouse Clones: Consequences for Pluripotency

, 2002
OCT4 DISTRIBUTION AND LEVEL IN MOUSE CLONES: CONSEQUENCES FOR PLURIPOTENCY Oct4 Distribution and Level in Mouse Clones: Consequences for Pluripotency (16) (-
Boiani, Michele Sigrid Eckardt Hans Scholer John McLaughlin
core   +1 more source

Bioinformatic Prediction of Novel microRNAs Encoded in Krüppel-like Factor 4 Gene [PDF]

Journal of Genetic Resources
MicroRNAs are small non-coding RNAs that can regulate gene expression that affects various cellular processes. Krüppel-like factor 4 (KLF4) is a transcription factor that has different regulatory functions, and it plays a role in various cellular ...
Mina Zahiri, Maryam Hassanlou, Amir-Reza Javanmard, Nooshin Bijari   +3 more
doaj   +1 more source

Sacral Neural Crest‐Derived Extracellular Vesicles Promote Pelvic Ganglion Repair Through Anti‐Pyroptotic Effects Involving ANXA1–FPR2 Signaling

Advanced Science, EarlyView.
Extracellular vesicles derived from human pluripotent stem cell‐derived sacral neural crest cells promote repair of injured major pelvic ganglia and improve colorectal function. These vesicles reduce oxidative stress and pyroptosis, with ANXA1–FPR2 signaling contributing to their protective effects, and show a favorable therapeutic profile compared ...
Ting Yang, Wenwei Liu, Hufei Wang, Yuxin Wu, Yuanchen Ma, Xiaofeng Wang, Tianxiang Lei, Qiao Tang, Yebohao Zhou, Weiqiang Li, Andy Peng Xiang, Wu Song   +11 more
wiley   +1 more source