Results 81 to 90 of about 1,247 (177)

An effective vaginal gel to deliver CRISPR/Cas9 system encapsulated in poly (β-amino ester) nanoparticles for vaginal gene therapy

EBioMedicine, 2020
Background: Gene therapy has held promises for treating specific genetic diseases. However, the key to clinical application depends on effective gene delivery.
Gang Niu, Zhuang Jin, Chong Zhang, Dan He, Xueqin Gao, Chenming Zou, Wei Zhang, Jiahui Ding, Bhudev C. Das, Konstantin Severinov, Inga Isabel Hitzeroth, Priya Ranjan Debata, Xin Ma, Xun Tian, Qinglei Gao, Jun Wu, Zeshan You, Rui Tian, Zifeng Cui, Weiwen Fan, Weiling Xie, Zhaoyue Huang, Chen Cao, Wei Xu, Hongxian Xie, Hongyan Xu, Xiongzhi Tang, Yan Wang, Zhiying Yu, Hui Han, Songwei Tan, Shuqin Chen, Zheng Hu   +32 more
doaj   +1 more source

The use of transgenic animals for xenotransplantation: An update

Animal Models and Experimental Medicine, Volume 8, Issue 11, Page 2041-2052, November 2025.
One way to produce genetically modified pigs is to use somatic cell nuclear transfer (SCNT). This procedure involves removing the metaphase plate from a mature oocyte using micromanipulation and then transferring the modified nucleus from the donor somatic cell to the oocyte.
Julia Motławska, Ana Amaral, Marta Cerveira‐Pinto, Paweł Kordowitzki   +3 more
wiley   +1 more source

OMICS Analyses in Xenotransplantation: Initial Findings, Key Precautions, and Virus Infections

Xenotransplantation, Volume 32, Issue 6, November/December 2025.
ABSTRACT OMICS analyses have the potential to greatly enhance our understanding of rejection and other processes in xenotransplantation. These approaches may therefore contribute to extending the survival time of xenotransplants. Initial OMICS studies in brain‐dead patients following transplantation of pig kidneys or hearts revealed increased ...
Joachim Denner
wiley   +1 more source

Virus safety of xenotransplantation and porcine endogenous retroviruses (PERVs)

, 2014
Introduction: The shortage of organs for patients is an issue that needs addressing. Xenotransplantation using pig cells or organs is the most favored option. However, pigs contain porcine endogenous retroviruses (PERV) in their genome which infect human cells. Like all retroviruses PERVs have immunosuppressive properties and may induce tumors.
openaire   +3 more sources

Modulation of Porcine Endogenous Retroviruses (PERVs) expression in vitro by shRNA in the presence of cyclosporine A and dexamethasone

Annals of Transplantation, 2012
Despite the fact that the risk of Porcine Endogenous Retroviruses (PERV) infection and propagation in human recipients is extremely low, such an event cannot be completely ruled out, especially in immunosuppressed patients. Therefore, the aim of this study was to analyze the expression of PERVs in vitro in the presence of immunosuppression agents ...
Daniel, Sypniewski, Ilona, Bednarek, Daria, Matczyńska, Sabina, Gałka, Tomasz, Loch, Dagna, Sołtysik, Grzegorz, Machnik, Ewa, Nowak   +7 more
openaire   +3 more sources

Production and Functional Verification of 8‐Gene (GGTA1, CMAH, β4GalNT2, hCD46, hCD55, hCD59, hTBM, hCD39)‐Edited Donor Pigs for Xenotransplantation

Cell Proliferation, Volume 58, Issue 9, September 2025.
Schematic representation of generation of 8‐gene‐edited cloned pigs and their verification by pig to non‐human primate kidney xenotransplantation. ABSTRACT Gene‐edited (GE) pig‐to‐human xenotransplantation continues to make breakthroughs, but which kind of gene combination is suitable for organ‐specific transplantation remains unclear.
Jiaoxiang Wang, Kaixiang Xu, Tao Liu, Heng Zhao, Muhammad Ameen Jamal, Gen Chen, Xiaoying Huo, Chang Yang, Deling Jiao, Taiyun Wei, Hanfei Huang, Hongfang Zhao, Jianxiong Guo, Fengchong Wang, Xiong Zhang, Kai Liu, Siming Qu, Gang Wang, Hui Guo, Gang Chen, Hong‐Ye Zhao, Zhong Zeng, Kefeng Dou, Hong‐Jiang Wei   +23 more
wiley   +1 more source

Xenotransplants: The Risks and Benefits [PDF]

, 2022
This literature review discusses how xenotransplants from animals, especially pigs, can serve as viable alternatives to allotransplants. There are many ways of ensuring the health of the host, including testing the donor pig for cytomegalovirus, Epstein ...
Sima Langner
core   +1 more source

Biocompatibility of Genetically‐Engineered Pig Cornea in Corneal Xenotransplantation: Preliminary In Vitro Study

Xenotransplantation, Volume 32, Issue 5, September/October 2025.
ABSTRACT Introduction We aimed to evaluate biophysical compatibility of corneal endothelial cells in genetically‐engineered (GE) pigs to identify the best candidate for in vivo nonhuman primate (NHP) study and to collect baseline data for future clinical trials from a biological function perspective.
Seung Hyeun Lee, Chung Young Kim, Jin Suk Ryu, Dong Hee Choi, Chang Ho Yoon, Chung‐Gyu Park, Kimyung Choi, Hyunil Kim, Mee Kum Kim   +8 more
wiley   +1 more source

An Evolutionarily Young Polar Bear (Ursus maritimus) Endogenous Retrovirus Identified from Next Generation Sequence Data

Viruses, 2015
Transcriptome analysis of polar bear (Ursus maritimus) tissues identified sequences with similarity to Porcine Endogenous Retroviruses (PERV). Based on these sequences, four proviral copies and 15 solo long terminal repeats (LTRs) of a newly described ...
Kyriakos Tsangaras, Jens Mayer, David E. Alquezar-Planas, Alex D. Greenwood   +3 more
doaj   +1 more source

Clinical Xenotransplantation of Gene‐Edited Pig Organs: A Review of Experiments in Living Humans Since 2022

Medicine Bulletin, Volume 1, Issue 1, Page 77-85, June 2025.
Recent clinical trials transplanted gene‐edited pig hearts, kidneys, and a liver into eight human patients, demonstrating xenotransplantation's potential to address organ shortages. While showing promise, outcomes varied, highlighting key challenges including immune rejection and managing patient comorbidities, requiring further optimization.
Lisha Mou, Zuhui Pu, David K. C. Cooper
wiley   +1 more source