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Comprehensive Mechanism of Gene Silencing and Its Role in Plant Growth and Development
Gene silencing is a negative feedback mechanism that regulates gene expression to define cell fate and also regulates metabolism and gene expression throughout the life of an organism.
Ahmed H. El-Sappah +22 more
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Transgene-induced gene silencing is not affected by a change in ploidy level. [PDF]
BACKGROUND: Whole genome duplication, which results in polyploidy, is a common feature of plant populations and a recurring event in the evolution of flowering plants.
Daniela Pignatta +4 more
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Bidirectional transcription directs both transcriptional gene activation and suppression in human cells. [PDF]
Small RNAs targeted to gene promoters in human cells have been shown to modulate both transcriptional gene suppression and activation. However, the mechanism involved in transcriptional activation has remained poorly defined, and an endogenous RNA ...
Kevin V Morris +4 more
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Virus‐Induced Gene Silencing, a Post Transcriptional Gene Silencing Method [PDF]
Virus‐induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA‐mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence‐specific manner ...
Unver, Turgay, Budak, Hikmet
openaire +4 more sources
Enhancement of post-transcriptional gene silencing by grafting [PDF]
We showed previously that grafting transmitted silencing occurred when transgenic ACC oxidase 1 (ACO1) overexpressing tomato plants that also produced siRNAs were grafted onto transgenic stocks that already showed strong silencing. The presence of siRNAs in these overexpressing scions may indicate that silencing, though inefficient, may already occur ...
Yuanhuai, Han, Donald, Grierson
openaire +2 more sources
Post-transcriptional regulation of several biological processes involved in latex production in Hevea brasiliensis [PDF]
Background Small RNAs modulate plant gene expression at both the transcriptional and post-transcriptional level, mostly through the induction of either targeted DNA methylation or transcript cleavage, respectively.
Julie Leclercq +13 more
doaj +2 more sources
RNA–DNA Interactions and DNA Methylation in Post-Transcriptional Gene Silencing [PDF]
Post-transcriptional gene silencing (PTGS) is a homology-dependent process that reduces cytoplasmic RNA levels. In several experimental systems, there is also an association of PTGS with methylation of DNA. To investigate this association, we used plants carrying a transgene encoding the green fluorescent protein (GFP). Gene silencing was induced using
L. Jones +5 more
semanticscholar +3 more sources
Flavonoid biosynthesis requires the activities of several enzymes, which form weakly-bound, ordered protein complexes termed metabolons. To decipher flux regulation in the flavonoid biosynthetic pathway of chrysanthemum (Chrysanthemum morifolium Ramat ...
Sun-Hyung Lim +5 more
doaj +1 more source
eIF4GI facilitates the MicroRNA-mediated gene silencing. [PDF]
MicroRNAs (miRNAs) are small noncoding RNAs that mediate post-transcriptional gene silencing by binding to complementary target mRNAs and recruiting the miRNA-containing ribonucleoprotein complexes to the mRNAs.
Incheol Ryu +4 more
doaj +1 more source
Epigenetic silencing in transgenic plants
Epigenetic silencing is a natural phenomenon in which the expression of gene is regulated through modifications of DNA, RNA or histone proteins. It is a mechanism for defending host genomes against the effects of transposable element, viral infection and
Sarma eRajeev Kumar +2 more
doaj +1 more source

