Results 21 to 30 of about 3,085 (163)
Pseudouridylation meets next-generation sequencing [PDF]
Methods, 2016 The isomerization of uridine to pseudouridine (Ψ), known as pseudouridylation, is the most abundant post-transcriptional modification of stable RNAs. Due to technical limitations in pseudouridine detection methods, studies on pseudouridylation have historically focused on ribosomal RNAs, transfer RNAs, and spliceosomal small nuclear RNAs, where Ψs play
Zaringhalam, Maryam +1 more
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A Tool to Design Bridging Oligos Used to Detect Pseudouridylation Sites on RNA after CMC Treatment
Non-Coding RNA, 2022 Pseudouridylation is one of the most abundant modifications found in RNAs. To identify the Pseudouridylation sites (Psi) in RNAs, several techniques have been developed, but the most common and robust is the CMC (N-cyclohexyl-N′-(2-morpholinoethyl ...
Baptiste Bogard +3 more
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Regulation of translation by ribosomal RNA pseudouridylation
Science Advances, 2023 Pseudouridine is enriched in ribosomal, spliceosomal, transfer, and messenger RNA and thus integral to the central dogma. The chemical basis for how pseudouridine affects the molecular apparatus such as ribosome, however, remains elusive owing to the lack of structures without this natural modification.
Yu Zhao, Jay Rai, Hong Li
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Frontiers in Genetics, 2023 Background: Prostate cancer (PCa) is one of the most common cancers in males around the globe, and about one-third of patients with localized PCa will experience biochemical recurrence (BCR) after radical prostatectomy or radiation therapy. Reportedly, a
Pengxiang Zheng +12 more
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Inducing nonsense suppression by targeted pseudouridylation [PDF]
Nature Protocols, 2012 Isomerization from uridine to pseudouridine (pseudouridylation) is largely catalyzed by a family of small ribonucleoproteins called box H/ACA RNPs, each of which contains one unique small RNA-the box H/ACA RNA. The specificity of the pseudouridylation reaction is determined by the base-pairing interactions between the guide sequence of the box H/ACA ...
Chao, Huang, Guowei, Wu, Yi-Tao, Yu
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Pseudouridines of tRNA Anticodon Stem-Loop Have Unexpected Role in Mutagenesis in Pseudomonas sp.
Microorganisms, 2020 Pseudouridines are known to be important for optimal translation. In this study we demonstrate an unexpected link between pseudouridylation of tRNA and mutation frequency in Pseudomonas species.
Mari Tagel +5 more
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Pseudouridylation goes regulatory [PDF]
The EMBO Journal, 2011 RNA modifications are omnipresent among non-coding RNAs, so much so that pseudouridine (an isomer of uridine) was originally designated the ‘fifth nucleoside’. Their role ranges from fine-tuning to being functionally essential to their target RNAs. Ever since their discovery some 60 years ago, RNA modifications were considered constitutive.
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RNA Pseudouridylation in Physiology and Medicine: For Better and for Worse [PDF]
Genes, 2017 Pseudouridine is the most abundant modification found in RNA. Today, thanks to next-generation sequencing techniques used in the detection of RNA modifications, pseudouridylation sites have been described in most eukaryotic RNA classes. In the present review, we will first consider the available information on the functional roles of pseudouridine(s ...
Penzo, Marianna +4 more
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The Role of Noncoding RNA Pseudouridylation in Nuclear Gene Expression Events
Frontiers in Bioengineering and Biotechnology, 2018 Pseudouridine is the most abundant internal RNA modification in stable noncoding RNAs (ncRNAs). It can be catalyzed by both RNA-dependent and RNA-independent mechanisms.
Yang Zhao +4 more
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snoRNPs: Functions in Ribosome Biogenesis
Biomolecules, 2020 Ribosomes are perhaps the most critical macromolecular machine as they are tasked with carrying out protein synthesis in cells. They are incredibly complex structures composed of protein components and heavily chemically modified RNAs.
Sandeep Ojha +2 more
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