GENCODE 2025: reference gene annotation for human and mouse. [PDF]
GENCODE produces comprehensive reference gene annotation for human and mouse. Entering its twentieth year, the project remains highly active as new technologies and methodologies allow us to catalog the genome at ever-increasing granularity.
Mudge JM +55 more
europepmc +2 more sources
Reference Gene Selection for Quantitative Gene Expression Analysis in Argynnis hyperbius [PDF]
Argynnis hyperbius (Lepidoptera: Nymphalidae), as an important environmental indicator species, has shown a gradual decline in its species richness amid intensifying climate change and increasing environmental pressures.
Hong-Juan Xin +6 more
doaj +2 more sources
qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (Litsea coreana) [PDF]
To identify stable reference genes for qRT-PCR analysis across different developmental stages and color variations of tender leaves in Litsea coreana, seven candidate reference genes were selected based on existing transcriptome data.
Qianli Dai +10 more
doaj +2 more sources
EF1α and αTUB Are Stable Reference Gene Pairs for RT-qPCR-Based Gene Expression Studies in Salix suchowensis Under Nitrogen Treatment Conditions [PDF]
Salix suchowensis is an ideal model organism for investigating nitrogen (N) transport mechanisms due to its low N-input requirements. Accurate quantification of gene expression is essential for elucidating these processes, with quantitative real-time PCR
Lei Huang +8 more
doaj +2 more sources
PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic–pituitary–gonadal axis in sows [PDF]
Objective The quantitative reverse transcription polymerase chain reaction (qPCR) is the most accurate and reliable technique for analysis of gene expression.
Hwan-Deuk Kim +10 more
doaj +1 more source
Antimicrobial resistance (AMR) is a significant public health threat. With the rise of affordable whole genome sequencing, in silico approaches to assessing AMR gene content can be used to detect known resistance mechanisms and potentially identify novel
M. Feldgarden +11 more
semanticscholar +1 more source
iRGvalid: A Robust in silico Method for Optimal Reference Gene Validation
BackgroundAppropriate reference genes are critical to accurately quantifying relative gene expression in research and clinical applications. Numerous efforts have been made to select the most stable reference gene(s), but a consensus has yet to be ...
Zhongxu Zhu, Keqin Gregg, Wenli Zhou
doaj +1 more source
The Gene Ontology's Reference Genome Project: a unified framework for functional annotation across species. [PDF]
The Gene Ontology (GO) is a collaborative effort that provides structured vocabularies for annotating the molecular function, biological role, and cellular location of gene products in a highly systematic way and in a species-neutral manner with the aim ...
Reference Genome Group of the Gene Ontology Consortium
doaj +1 more source
Background Sexually transmitted infections (STIs) are prevalent throughout the world and impose a significant burden on individual health and public health systems.
Zhihai Ma +12 more
doaj +1 more source
The tomato leaf miner, Tuta absoluta is a destructive pest of tomato. The leaf-mining activities of its larvae can cause significant yield losses. Real-time quantitative polymerase chain reaction (RT-qPCR) is commonly used to measure gene expression, and
Xin Yan +4 more
doaj +1 more source

