Effective Alu repeat based RT-qPCR normalization in cancer cell perturbation experiments [PDF]
PLoS ONE, 2013 Background: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories.
Beckers, Anneleen +13 more
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Reference Gene Selection for Quantitative Gene Expression Analysis in Argynnis hyperbius [PDF]
InsectsArgynnis hyperbius (Lepidoptera: Nymphalidae), as an important environmental indicator species, has shown a gradual decline in its species richness amid intensifying climate change and increasing environmental pressures.
Hong-Juan Xin +6 more
doaj +2 more sources
qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (Litsea coreana) [PDF]
Current Issues in Molecular BiologyTo identify stable reference genes for qRT-PCR analysis across different developmental stages and color variations of tender leaves in Litsea coreana, seven candidate reference genes were selected based on existing transcriptome data.
Qianli Dai +10 more
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EF1α and αTUB Are Stable Reference Gene Pairs for RT-qPCR-Based Gene Expression Studies in Salix suchowensis Under Nitrogen Treatment Conditions [PDF]
PlantsSalix suchowensis is an ideal model organism for investigating nitrogen (N) transport mechanisms due to its low N-input requirements. Accurate quantification of gene expression is essential for elucidating these processes, with quantitative real-time PCR
Lei Huang +8 more
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PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic–pituitary–gonadal axis in sows [PDF]
Animal Bioscience, 2022 Objective The quantitative reverse transcription polymerase chain reaction (qPCR) is the most accurate and reliable technique for analysis of gene expression.
Hwan-Deuk Kim +10 more
doaj +1 more source
iRGvalid: A Robust in silico Method for Optimal Reference Gene Validation
Frontiers in Genetics, 2021 BackgroundAppropriate reference genes are critical to accurately quantifying relative gene expression in research and clinical applications. Numerous efforts have been made to select the most stable reference gene(s), but a consensus has yet to be ...
Zhongxu Zhu, Keqin Gregg, Wenli Zhou
doaj +1 more source
The Gene Ontology's Reference Genome Project: a unified framework for functional annotation across species. [PDF]
PLoS Computational Biology, 2009 The Gene Ontology (GO) is a collaborative effort that provides structured vocabularies for annotating the molecular function, biological role, and cellular location of gene products in a highly systematic way and in a species-neutral manner with the aim ...
Reference Genome Group of the Gene Ontology Consortium
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Infectious Agents and Cancer, 2022 Background Sexually transmitted infections (STIs) are prevalent throughout the world and impose a significant burden on individual health and public health systems.
Zhihai Ma +12 more
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Insects, 2021 The tomato leaf miner, Tuta absoluta is a destructive pest of tomato. The leaf-mining activities of its larvae can cause significant yield losses. Real-time quantitative polymerase chain reaction (RT-qPCR) is commonly used to measure gene expression, and
Xin Yan +4 more
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Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes
Frontiers in Physiology, 2021 Reverse transcription quantitative real-time PCR is the most commonly used method to detect gene expression levels. In experiments, it is often necessary to correct and standardize the expression level of target genes with reference genes.
Wei Na +24 more
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