Results 91 to 100 of about 13,061,353 (246)

Postmenopausal Osteoporosis reference genes for qPCR expression assays [PDF]

Scientific Reports, 2019
AbstractOsteoporosis (OP) is a multifactorial disease influenced by genetic factors in more than half of the cases. In spite of the efforts to clarify the relationship among genetic factors and susceptibility to develop OP, many genetic associations need to be further functionally validated.
Camilla Albertina Dantas de Lima, Suelen Cristina de Lima, Alexandre Domingues Barbosa, Paula Sandrin-Garcia, Will de Barros Pita, Jaqueline de Azevêdo Silva, Sergio Crovella   +6 more
openaire   +4 more sources

A full-length S1 gene sequencing of a novel emerged GI-19 and GI-23 lineages of Infectious bronchitis virus currently circulating in chicken flocks in upper Egypt reveals marked genetic diversity and recombination events

Virology Journal
Background Infectious bronchitis virus (IBV) is a highly contagious evolving pathogen that causes respiratory, urinary and reproductive symptoms; threatening the poultry industry globally.
Eman Shosha, Sara Abdelnaser, Ali Mahmoud Zanaty   +2 more
doaj   +1 more source

How to perform RT-qPCR accurately in plant species?: a case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population [PDF]

, 2013
Background: Flower colour variation is one of the most crucial selection criteria in the breeding of a flowering pot plant, as is also the case for azalea (Rhododendron simsii hybrids).
De Keyser, Ellen, De Riek, Jan, Desmet, Laurence, Van Bockstaele, Erik   +3 more
core   +2 more sources

Reference gene stability of a synanthropic fly, Chrysomya megacephala

Parasites & Vectors, 2015
BackgroundStable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction (qPCR).
Xiaoyun Wang, M. Xiong, Jialu Wang, C. Lei, F. Zhu   +4 more
semanticscholar   +1 more source

Tissue resolved, gene structure refined equine transcriptome. [PDF]

, 2017
BackgroundTranscriptome interpretation relies on a good-quality reference transcriptome for accurate quantification of gene expression as well as functional analysis of genetic variants.
Bellone, RR, Brown, CT, Finno, CJ, Mansour, TA, Mienaltowski, MJ, Murray, JD, Penedo, MC, Ross, PJ, Scott, EY, Valberg, SJ   +9 more
core   +1 more source

Reference Gene Validation for RT-qPCR, a Note on Different Available Software Packages

PLoS ONE, 2015
Background An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR).
W. De Spiegelaere, Jutta Dern-Wieloch, Roswitha Weigel, V. Schumacher, H. Schorle, D. Nettersheim, M. Bergmann, R. Brehm, S. Kliesch, L. Vandekerckhove, C. Fink   +10 more
semanticscholar   +1 more source

Housekeeping Genes for Parkinson’s Disease in Humans and Mice

Cells, 2021
A critical aspect of real-time PCR is the presence of housekeeping genes (HKGs) as an internal control for the normalization of expression data for genes of interest.
Anelya Kh. Alieva, Elena V. Filatova, Margarita M. Rudenok, Petr A. Slominsky, Maria I. Shadrina   +4 more
doaj   +1 more source

Evolution of gene families involved in banana fruit development and ripening : W077 [PDF]

, 2013
A reference genome sequence of banana was recently obtained from a Musa acuminata doubled-haploid accession (DH-Pahang, 523 Mb) and organized into eleven pseudomolecules. This genome opens brand new perspectives for the identification of genes underlying
Cardi, Céline, D'Hont, Angélique, Dasilva, C., Jourda, Cyril, Labadie, Karine, Mbéguié-A-Mbéguié, Didier, Yahiaoui, Nabila   +6 more
core  

Gene Expression Commons: an open platform for absolute gene expression profiling. [PDF]

, 2012
Gene expression profiling using microarrays has been limited to comparisons of gene expression between small numbers of samples within individual experiments.
Bhattacharya, Deepta, Dill, David L, Ehrlich, Lauren IR, Fathman, John W, Inlay, Matthew A, Rossi, Derrick J, Sahoo, Debashis, Seita, Jun, Serwold, Thomas, Weissman, Irving L   +9 more
core   +2 more sources

Identification and Validation of Reference Genes for Gene Expression Analysis in Schima superba

Genes, 2021
Real-time quantitative PCR (RT-qPCR) is a reliable and high-throughput technique for gene expression studies, but its accuracy depends on the expression stability of reference genes. Schima superba is a fast-growing timber species with strong resistance.
Zhongyi Yang, Rui Zhang, Zhichun Zhou
semanticscholar   +1 more source