Frontiers in Plant Science, 2017 Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants.
Tao Li +5 more
doaj +1 more source
Retrotransposition of gene transcripts leads to structural variation in mammalian genomes [PDF]
, 2013 BACKGROUND: Retroposed processed gene transcripts are an important source of material for new gene formation on evolutionary timescales. Most prior work on gene retrocopy discovery compared copies in reference genome assemblies to their source genes ...
Ballinger, Tracy J +7 more
core +3 more sources
Pseudohypoaldosteronism type 1 due to novel variants of SCNN1B gene. [PDF]
, 2016 UnlabelledAutosomal recessive pseudohypoaldosteronism type 1 (PHA1) is a rare disorder characterized by sodium wasting, failure to thrive, hyperkalemia, hypovolemia and metabolic acidosis.
Abraham, Smita B +9 more
core +2 more sources
A gene-based association method for mapping traits using reference transcriptome data
Nature Genetics, 2015 Genome-wide association studies (GWAS) have identified thousands of variants robustly associated with complex traits. However, the biological mechanisms underlying these associations are, in general, not well understood.
E. Gamazon +10 more
semanticscholar +1 more source
The impact of imprecisely measured covariates on estimating gene-environment interactions [PDF]
, 2006 BACKGROUND The effects of measurement error in epidemiological exposures and confounders on estimated effects of exposure are well described, but the effects on estimates for gene-environment interactions has received rather less attention.
Cade, J.E. +2 more
core +2 more sources
Hypoxanthine guanine phosphoribosyl transferase is the most stable reference gene for gene expression analysis by quantitative PCR in peripheral blood mononuclear cells from women with the polycystic ovary syndrome [PDF]
Journal of Medical Biochemistry, 2014 Background: The polycystic ovary syndrome (PCOS) is a frequent endocrine disorder that affects women of reproductive age. As the syndrome is strongly associated with obesity, it is of interest to examine the gene expression differences that accompany its
Vojnović-Milutinović Danijela +6 more
doaj
Animals, 2019 Investigating the critical genes related to milk synthesis is essential for the improvement of the milk yield of the yak. Real-time quantitative polymerase chain reaction (RT-qPCR) is a reliable and widely used method to measure and evaluate gene ...
Xiaoyun Wu +9 more
doaj +1 more source
TREEOME: A framework for epigenetic and transcriptomic data integration to explore regulatory interactions controlling transcription [PDF]
, 2015 Motivation: Predictive modelling of gene expression is a powerful framework for the in silico exploration of transcriptional regulatory interactions through the integration of high-throughput -omics data.
Budden, David M +2 more
core +2 more sources
Influence of reference genes on expression of TYR, MITF and ASIP genes in tissues of Silky Fowl
浙江大学学报. 农业与生命科学版, 2015 Silky Fowl is a precious local breed containing a large amount of melanin. Therefore, it is considered to have high edible and medicinal value. Study on the related gene expression is helpful to reveal the internal regulation mechanism of melanin ...
Zheng Nenzhu +7 more
doaj +1 more source
BMC Genomics, 2014 BackgroundGene expression analysis by reverse transcription quantitative PCR (qPCR) is the most widely used method for analyzing the expression of a moderate number of genes and also for the validation of microarray results.
D. Robledo +6 more
semanticscholar +1 more source