Results 111 to 120 of about 2,306,220 (297)

The role and implications of mammalian cellular circadian entrainment

FEBS Letters, EarlyView.
At their most fundamental level, mammalian circadian rhythms occur inside every individual cell. To tell the correct time, cells must align (or ‘entrain’) their circadian rhythm to the external environment. In this review, we highlight how cells entrain to the major circadian cues of light, feeding and temperature, and the implications this has for our
Priya Crosby
wiley   +1 more source

Correction to: Custom selected reference genes outperform pre-defined reference genes in transcriptomic analysis [PDF]

BMC Genomics, 2021
dos Santos, K. C. G., Desgagné-Penix, I., Germain, H.   +2 more
openaire   +3 more sources

Molecular bases of circadian magnesium rhythms across eukaryotes

FEBS Letters, EarlyView.
Circadian rhythms in intracellular [Mg2+] exist across eukaryotic kingdoms. Central roles for Mg2+ in metabolism suggest that Mg2+ rhythms could regulate daily cellular energy and metabolism. In this Perspective paper, we propose that ancestral prokaryotic transport proteins could be responsible for mediating Mg2+ rhythms and posit a feedback model ...
Helen K. Feord, Gerben van Ooijen
wiley   +1 more source

Expression of Nitrilases in Brassica juncea var. tumida Tsen in Root Galls Caused by Plasmodiophora brassicae

Journal of Integrative Agriculture, 2012
Five commonly-used reference genes: ACT (actin), UBE (ubiquitin-conjugating enzyme), RPL2 (ribosomal protein L2), BRP II (RNA polymerase II subunit), and NADH (nicotinamide adenine dinucleotide) were examined using geNorm software as reference genes for ...
Yan LIU, You-ping YIN, Zhong-kang WANG, Yuan-li LUO   +3 more
doaj   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Probabilities of spurious connections in gene networks: Application to expression time series

, 2004
Motivation: The reconstruction of gene networks from gene expression microarrays is gaining popularity as methods improve and as more data become available.
Bickel, D. R. Bickel, de Jong, Efron, Fernando, Pepe   +5 more
core   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Tissue-specific validation of reference genes for accurate qRT-PCR analysis during sex differentiation in Amphiprion ocellaris [PDF]

Brazilian Journal of Biology
Quantitative real-time PCR (qRT-PCR) is a widely used method for measuring gene expression, but its reliability depends on using stable reference genes for normalization.
C. Boonphakdee, J. Thamnawasolos, T. Boonphakdee, T. Mataroepayotorn   +3 more
doaj   +1 more source

Reference Standards for Gene and Cell Therapy Products [PDF]

Molecular Therapy, 2017
Reference standards are important tools for the calibration of medical products and procedures. They are particularly important when dosing and potency are critical parameters for patient safety and product efficacy.1 Reference standards benefit the field by fostering best practices for the manufacturing and testing of safe and efficacious medicinal ...
openaire   +2 more sources

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source